Effect of Mono-ammonium phosphate 12-61 and salinity on growth, total chlorophyll and proline content of bean Vicia faba L minor, Variety Sidi Aich

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Effect of Mono-ammonium phosphate 12-61 and salinity on growth, total chlorophyll and proline content of bean Vicia faba L minor, Variety Sidi Aich

Nouri Tayeb, Reguieg Yssaad Houcine Abdelhakim, Latigui Ahmed, Oubachir Ahlem, Djebbar Naziha, Bouyahia Hadj
Int. J. Biosci.11( 2), 163-169, August 2017.
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Soil salinity is a major constraint to agricultural development in the whole world, particularly in arid and semi-arid areas. The purpose of this study is to evaluate the combined effect of four salinity levels 0, 3, 6, 9dS/m and three concentrations of Monoammonium phosphate (MAP) 12-61 on Growth, biochemical’s parameters as total Chlorophyll and the content proline of bean Viciafaba L minor, variety of SIDI AICH. Sowing was carried on plastic cylinders of 40cm in height containing soil of different salinity levels (0.3.6 and 9dS/m), and treated with Mono-Ammonium Phosphate 12.61 at doses of 1 and 1.5g/ 5kg of dry soil. However, The Irrigation of plants was performed with distilled water and a Hoagland nutrient solution. Also, Saline and MAP effect were investigated on leaf and root area, stem and root length, total chlorophyll and proline content. So, the results showed that; the strong salinity of 9dS/m caused a reduction in the length of the stems and roots, the leaf and root area, total chlorophyll. Nevertheless, the treatment of plants by the MAP at the dose of 1 and 1.5g was significantly improved leaf and root area, stem length and proline content under the effect of salinity.


Abdul Qados AMS. 2011. Effect of salt stress on plant growth and metabolism of bean plant Vicia faba L. Journal of the Saudi Society of Agricultural Sciences 10, 7-15. https://doi.org/10.1016/j.jssas.2010.06.002.

Afroz S, Firoz M, Hayat S, Siddiqui MH. 2005. Exogenous application of gibberellic acid counteracts the ill effect of sodium chloride in mustard. Turkish journal of biology 29, 233-236.

Baatour O, M’rah S, Ben Brahim N, Boulesnem F, Lachaal M. 2004. Physiological response of the gestation (Lythyrus sativus) to the salinity of the environment. Review of the arid regions 1, 346-358.

Benbrahim KF, Ismaili M, Benbrahim SF, Tribak A. 2004. Problèmes de dégradation de l’environnement par la désertification et la déforestation: impact du phénomène au Maroc. Science et changements planétaires/Sécheresse 15(4), 307-320.

Benidire L, Daoui K, Fatemi ZA, Achouak W, Bouarab L, Oufdou K. 2015. Effect of salt stress on germination and seedling of Vicia faba L. Journal of Materials and Environmental Science 6(3), 840-851.

Bergman I, Loxley R. 1970. New spectrometric method for the determination of proline in tissues hydrolysates. Analytical Chemistry 42, 702-706.

El Houcine. 2000. National program for the transfer of technology in agriculture, Bultin.Institue of agricutural and veterinary. University of hassan II.

El midaoui M, Benbella M, Aït houssa A, Ibriz M, Talouizte A. 2007. Contribution to the study of some mechanisms of adaptation to salinity in cultivated sunflower (Helianthus annuus L.). Review Hommes Terre et Eaux 136, 29-34.

Ingweye JN, Kalio GA, Ubua JA, Umoren EP. 2010. Nutritional Evaluation of Wild Sicklepod (Senna obtusifolia) Seeds from Obanliku, South-Eastern Nigeria. American Journal of Food Technology 5, 1-12 http://scialert.net/abstract/?doi=ajft.2010.1.12.

Jouve P, Corbier-Bartuaux C, Cornet A. 2002. Combating Desertification in Development Projects. A Scientific Perspective on the SFM Experience in Sub-Saharan Africa and the Maghreb Mimosa (Eds). French Development Agency (AFD) and French Scientific Committee on Desertification CSFD 162 p.

Kasmi A, Latigui A, Mettai K, Sahli B, Dilem A. 2012. Use of Sewage sludge and Fiber palm Co-compost as Components of Substrates Lycopersicum esculentum and Cucumismelo cultivated in Soil less crop. American journal of plant Physiology 7(2), 97-103. https://doi.org/10.3923/ajpp.2012.97.103.

Manchanda G, Garg N. 2008. Salinity and its effects on the functional biology of legumes. Acta Physiologica Plant 30, 595-618. https://doi.org/10.1007/s11738-008-0173-3.

Misra N, Ansari MS, Gupta AK. 2006. Differential response of scavenging of reactive oxygen species in green gram genotype grown under salinity stress. American journal of plant physiology 1, 41-53 https://doi.org/10.1016/j.plantsci.2005.02.013.

Murillo-Amador B, Yamada S, Yamaguch T, Puente ER, Serrano NA, Hernandez LG, Aguilar RL, Dieguez ET, Garibay AN. 2007. Salinity toxicity influence of calcium silicate on growth physiological parameters and mineral nutrition in two legume species under salt stress, Journal of Agronomy and Crop Science 193(6), 413-421. https://doi.org/ 10.1111/j.1439-037X.2007.00273.x.

Netting A. 2002. pH, abscissic acide and the integration of metabolism in plants under stressed and non –stressed conditions –II-Modifiaction in mode of metabolism induced by variation in the tension of the water column and stress. Journal of Experimental Botany 53(356), 151-173.

Nouri T. 2012. Response of the bean vicia faba L to saline stress, case of asandysoilmodified by bentonite. Magister thesis, university of Mostaganem Algeria.

Okçu G, Kaya MD, Atak M. 2005. Effect of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.). turkish journal of agriculture and forestry 29, 237- 242.

Pierzynski GM. 1991. The chemistry and mineralogy of phosphorus in excessively fertilized soils. Critical reviews in environmental science and technology 21, 265-295.

Quadir M, Coster JD. 2004. Crop and irrigation management strategies for saline-sodic soils and waters aimes at environmentally sustainable agricultures. Science of the Total Environment 323, 1-19. https://doi.org/10.1016/j.scitotenv.2003.10.012.

Reguieg Yssaad HA, Latigui A, Nouri T, Bessafi L. 2012. Effect of salt stress and bentonite on the germination and proline content of Vicia faba L. Plant var. ‘Semilla violeta’ and ‘Reine mora’.American journal of plant physiology 7, 212-219. http://scialert. net/abstract/?doi=ajpp.2012.212.219.

Rengasamy P. 2010. Soil processes affecting crop production in salt affected soils. Functional Plant Biology 37(7), 613-620. https://doi.org/10.1071 /FP09249

Rontain D, Basset G, Hanson AD. 2002. Metabolic engineering of osmoprotectant accumulation in plants. Metabolic engineering 4, 49-56. https://doi.org/10. 1006/mben.2001.0208 

Seeman JR, Criteheey C. 1985. Effect of salt stress on the growth, ion content, Phaseo/us vulgaris L. Planta 164, 151-162.  https://doi.org/10.1007/BF00396077

Sen S. 2010. S-nitrosylation process acts as a regulatory switch for seed germination in wheat. American Journal of Plant Physiology 5, 122-132. https://doi.org/10.3923/ajpp.2010.122.132.

Sultana N, Ikeda T, Itoh R. 1999. Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains. Environmental and Expérimental Botany journal 42, 211-220.

Taffouo VD, Wamba OF, Yombi E, Nono GV, Akoe A. 2010. Growth, yield, water status and ionic distribution response of three bambara groundnut (Vigna subterranean (L.) verdc.) landraces grown under saline conditions, International Journal of Botany 6(1), 53-58. http://dx.doi.org/10.3923/ijb.2010.53.58.

Tort NB, Turkyilmaz A. 2004. Physiological investigation on the mechanisms of salinity tolerance in some barley culture forms. Journal Faculty Science Ege University 27, 1-16.

Wang Y, Nil N. 2000. Changes in chlorophyll, ribulose biphosphate carboxylase oxygenase, glycine betaine content, photosynthesis and transpiration in Amaranthus tricolor leaves during salt stress. Journal of Horticultural Science and Biotechnology 75, 623-627.

Zhu JK. 2001. Plants salt tolerant. Trends Plant Science 6, 66-71