Effect of Moringa oleifera leaves on the growth and enzymatic activities of Labeo rohita by replacing with fish meal

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Research Paper 01/01/2018
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Effect of Moringa oleifera leaves on the growth and enzymatic activities of Labeo rohita by replacing with fish meal

Zahoor Ahmed Khetran, Zohaib Noor, Fayyaz Rasool, Muhammad Naveed Aslam, Zulfiqar Ali Rahujo, Hussain Mehdi, Abdul Sattar Khetran, Muhammad Rafique Khetran, Karim Johar Khan, Muhammad Ahmad, Mannal Noor
Int. J. Biosci.12( 1), 200-205, January 2018.
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Moringa oleifera (M. oleifera) is the most common source of plant proteins in fish diet, because it is cheap source of protein and easily availbale.The present study was designed to investigate the effect of (M. oleifera) on the growth and enzymatic activities of Labeo rohita (L. rohita) supplemented diet of Moringaoleifera (M.oleifera)leaves, experiment was conducted in four groups i.e. controll group (no M. oleiferasupplemets) and experimental groups T1 (15%), T2 (30%), T3 (45%). All the fish were reared in the glass aquaria in the replicate of three. After the completion of experimetal trial fish weight were noticed, maximum weight gain (15.23 ± 0.08) was observed in the T2 group compared to other groups. After analyzing the amylase(0.55 ± 0.07) and cellulase activties (0.53 ± 0.09) was high in T2 compared to other experimental groups, which results in efficent digestion. All the water quality paramters were within range. Thus it is concluded from the present study that repacing 30% (M.oleifera) with fish meal can increase the growth rate and enzymatic actvities of (L. rohita).


Abi M, Ahmed MS. 2009.Growth response of Labeo rohita fingerlings with different feeding regimes under intensive rearing. The Journal of Animal and Plant Sciences 19, 45-49.

Abo-state Hnan, Hammouda, Yasser, EL-Nadi Ali, Abozaid, Hesham. 2015. Evaluation of Feeding Raw Moringa (Moringa oleifera). Leaves Meal in Nile Tilapia Fingerlings Oreochromis niloticus Diets. Global Veterinaria 13, 105-111.

Afuang W, Siddhuraju P, Becker K. 2003. Comparative nutritional evaluation of raw, methanol extracted residues and methanol extracts of Moringa (Moringa oleiera Lam.) leaf on growth performance and feed utilization in Nile tilapia (Oreochromis niloticus L.). Aquaculture. Research 34, 1147-1159. https://doi.org/10.1046/j.13652109.2003.00920.x

Ali SM, El-Nadi, Mohamed K, Khames. 2015. Effect of Dietary Supplementation with Moringa oleifara Leaves on Growth Performance Parameters on Nile Tilapia Oreochromis niloticus. Research Journal of Fisheries and Hydrobiology 10, 10-14.

Ayotunde E, Ofem BO. 2008. Acute and chronic toxicity of pawpaw Carica papaya seed Powder to adult Nile tilapia Oreochromis niloticus. African. Journal of Biotechnology 7, 22675-32274.

Bernfeld P. 1955. Amylase Apha and Beta. Method in Enzymology 1, 149-158. https://doi.org/10.1016/0076-6879(55)01021-5

Denison D, Koehn R. 1977. Cellulase activity of Poronia oedipus. Mycologia 592-603. https://doi.org/10.2307/3758562 

Foidl NHPS, Makkar Becker K. 2001. The potential of Moringa oleifera for agricultural and industrial uses. In: L. J. Fuglie, [Ed.]. The Miracle Tree. CTA, Wugeningen, Netherlands and CWS, New York, USA, 45-77.

Hardy RW. 2010. Utilization of plant proteins in fish diets: effects of global demand and supplies of fish meal. Aquaculture Research. 41, 770e776. https://doi.org/10.1111/j.13652109.2009.02349.x

Ibrar M, Zuberi A, Amir I, Imran M, Noor Z. 2017. Effect of Probiotic Geotrichum candidumon Early Rearing of Labeo rohita (Hamilton, 1822). Turkish Journal of Fisheries and Aquatic Sciences 17, 1263-1270. https://doi.org/10.4194/1303-2712-v17_6_19

Kittisak Puycha, Bundit Yuangsoi, Siripavee Charoenwattanasak, Sutee Wongmaneeprateep, Pornthep Niamphithak, Pattama Wiriyapattanasub. 2017. Effect of moringa (Moringa oleifera) leaf supplementation on growth performance and feed utilization of Bocourti’s catfish (Pangasius bocourti). Agriculture and Natural Resources 1-6. https://doi.org/10.1016/j.anres.2017.10.001

Richter N, Siddhuraju P, Becker K. 2003. Evaluation of the quality of (Moringa oleifera Lam.) leaf as an alternative protein source for Nile tilapia (Oreochromis niloliticus L.). Aquaculture 217, 599-611. https://doi.org/10.1016/s0044-8486(02)00497-0

Makkar HPS, Becker K. 1996. Nutritional value and antinutritional components of whole and ethanol extracted Moringa oleifera leaf. Anim. Animal Feed Science and Technology 63, 211-228. https://doi.org/10.1016/s0377-8401(96)01023-1

Makkar HPS, Becker K. 1997. Nutrient and antiquality factors in different morphological parts of Moringa oleifera tree. Journal of Agricultural Science128, 311-322. https://doi.org/10.1017/s0021859697004292