Effect of plant hormones on callus induction of explant types in endangered medicinal herb Tashnedari
Paper Details
Effect of plant hormones on callus induction of explant types in endangered medicinal herb Tashnedari
Abstract
Tashnedari endangered medicinal with Scrophularia striata scientific name is native to Iran and its main habitat is Ilam province. In order to callus induction in this plant species two separate factorial experiment as completely randomized design with three replications was performed in Sari agricultural sciences and natural resources university biotechnology laboratory in 2012. Explants were prepared as stem and leaf. And effect of BAP and 2, 4-D hormones in MS medium was studied at four levels on its callus induction. According to the results, combined of hormones containing 1.5 mg.L-1 BAP and 1.5 mg.L-1 2-4-D were introduced as the best treatment for callus induction ( = 52.22 %) using stem explants, and 1.5 mg.L-1 BAP and 2.5 mg.L-1 2-4 D as the best treatment for callus induction ( = 76.66 %) using leaf explants. Callus fresh weight was measured for stem explant And the freshest weight was observed in 3.5 mg.L-1 BAP and 1.5 mg.L-1 2, 4-D hormone compound.
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