Effect of Rhizobium and Phosphate Solubilizing Bacteria at different levels of Phosphorus applied on Nodulation, Growth and Yield of Peas (Pisum Sativum)

Paper Details

Research Paper 01/05/2016
Views (445) Download (13)
current_issue_feature_image
publication_file

Effect of Rhizobium and Phosphate Solubilizing Bacteria at different levels of Phosphorus applied on Nodulation, Growth and Yield of Peas (Pisum Sativum)

Kulsoom Abid, Tariq Sultan, Muhammad Zahid Kiani, Shahbaz Ahmad, Tauseef Tabassam, Mahmood-ul-Hassan
Int. J. Biosci.8( 5), 112-121, May 2016.
Certificate: IJB 2016 [Generate Certificate]

Abstract

Bacterial inoculation of Rhizobium species tend to improve the root architecture of legume crop through their potential of colonization and nitrogen fixing ability. Co-inoculation of Rhizobium and phosphate solubilizing bacteria (PSB) strain will further improve the nutrient availability to plant. This study was conducted to evaluate the response of peas to Rhizobium and PSB inoculation individually and together at 20 and 40 mg kg-1 phosphorus (P) application in-vitro and pot culture experiment. Recommended fertilizer dose was an additional treatment. The co-inoculation of Rhizobium + PSB at 40 mg P kg-1 soil significantly increased shoot and root length, number of flowers, pod, and nodules per plant, root and shoot dry weight, 100 grain weight and number of grains per pod up to 37, 25, 60, 220, 25, 125, 34, 19 and 20%, respectively over 20 mg kg-1 P application. Similarly, N and P concentration of straw and grain as well as soil N and P contents after harvesting was also significantly increase by the co-inoculation of nitrogen fixing and PSB compared to single inoculation at 20 mg kg-1 P application. Results showed that co-inoculation at 20 and 40 mg kg P application can improve the nodulation, growth parameters, yield and nutrient concentrations and uptake.

VIEWS 18

Anderson JM, Ingram JSI. 1993. Tropical soil biology and Fertility: A hand Book of method, CAB international, Wallingford, UK.

Adesemoye A, Torbert H, Kloepper J. 2008. Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system. Canadian journal of microbiology 54, 876-886. http://dx.doi.org/10.1139/w08-081

Adesemoye AO, Kloepper JW. 2009. Plant– microbes interactions in enhanced fertilizer-use efficiency. Applied microbiology and biotechnology 85, 1-12. http://dx.doi.org/10.1007/s00253-009-2196-0.

Ahemad M, Khan MS. 2010. Comparative toxicity of selected insecticides to pea plants and growth promotion in response to insecticide-tolerant and plant growth promoting Rhizobium leguminosarum. Crop protection 29, 325-329. http://dx.doi.org/10.1016/j.cropro.2010.01.005

Belimov A, Kojemiakov A, Chuvarliyeva CN. 1995. Interaction between barley and mixed cultures of nitrogen fixing and phosphate-solubilizing bacteria. Plant and Soil 173, 29-37.

Berg G. 2009. Plant–microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture. Applied Microbiology and Biotechnology 84, 11-18. http://dx.doi.org/10.1007s00253-009-2092-7

Chabot R, Antoun H, Cescas MP. 1996. Growth promotion of maize and lettuce by phosphate-solubilizing Rhizobium leguminosarum biovar. phaseoli. Plant and Soil 184, 311-321.

Chen C, Condron L, Davis M, Sherlock R. 2002. Phosphorus dynamics in the rhizosphere of perennial ryegrass, Lolium perenne L. and radiata pine, Pinus radiata. Soil Biology and Biochemistry 34, 487-499. http://dx.doi.org/10.1104/pp.111.175448

Dobbelaere S, Vanderleyden J, Okon Y. 2003. Plant growth-promoting effects of diazotrophs in the rhizosphere. Critical Reviews in Plant Sciences 22, 107-149. http://dx.doi.org/ 10.1080/713610853

Elhassan GA, Abdelgani ME, Osman AG, Mohamed SS, Abdelgadir BS. 2010. Potential production and application of biofertilizers in Sudan. Pakistan Journal of Nutrition 9, 926-934.

Heanes D. 1984. Determination of total organic‐C in soils by an improved chromic acid digestion and spectrophotometric procedure. Communications in Soil Science & Plant Analysis 15, 1191-1213. http://dx.doi.org/10.1080/00103628409367551

Hickling D. 2003. Canadian feed peas industry guide. Pulse Canada, Winipeg, MB, Canada, 35 p.

Huang H, Erickson R. 2007. Effect of Seed Treatment with Rhizobium leguminosarum on Pythium Damping‐off, Seedling Height, Root Nodulation, Root Biomass, Shoot Biomass, and Seed Yield of Pea and Lentil. Journal of phytopathology 155, 31-37. http://dx.doi.org/10.1111/j.1439-0434.2006.01189.

Khurana A, Sharma P. 2000. Effect of dual inoculation of phosphate solubilizing bacteria, Bradyrhizobium sp.(cicer) and phosphorus on nitrogen fixation and yield of chickpea. Indian Journal of Pulses Research 13, 66-67.

Klute A. 1986. Methods of soil analysis. Part 1. Physical and mineralogical methods, American Society of Agronomy, Inc.

Kosar HS, Gill M, Aziz T, Akhtar M, Imran M. 2002. Solublization of tri-calcium phosphate by different wheat genotypes. Pakistan Journal of Agricultural Sciences 39, 273-277.

Linu M, Stephen J, Jisha M. 2009. Phosphate solubilizing Gluconacetobacter sp., Burkholderia sp. and their potential interaction with cowpea (Vigna unguiculata (L.) Walp.). International Journal of Agricultural Research 4, 79-87.

McLean E. 1982. Soil pH and lime requirement. Methods of soil analysis. Part 2. Chemical and microbiological properties, 199-224.

Mirza BS, Mirza MS, Bano A, Malik KA. 2007. Coinoculation  of  chickpea  with  Rhizobium  isolates from roots and nodules and phytohormone-producing Enterobacter strains. Animal Production Science 47, 1008-1015. http://dx.doi.org/10.1071/EA06151

Nisar M, Ghafoor A, Ahmad H. 2008. Evaluation of genetic diversity of pea germplasm through phenotypic trait analysis. Pak. J. Bot 40, 2081-2086.

Page AL. 1982. Methods of soil analysis. Part 2. Chemical and microbiological properties, American Society of Agronomy, Soil Science Society of America.

Pal SS. 1998. Interactions of an acid tolerant strain of phosphate solubilizing bacteria with a few acid tolerant crops. Plant and soil 198, 169-177.

Pepper I. 2000. Beneficial and pathogenic microbes in agriculture. Environmental Microbiology, 425-446. Perveen S, Saghir Khan M, Zaidi A. 2002. Effect of rhizospheric micro-organisms on growth and yield of greengram (Phaseolus radiatus). Indian journal of agricultural science 72, 421-423.

Pikovskaya R. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya 17, 362-370.

Rokhzadi A, Toashih. 2011. Nutrient uptake and yield of chickpea (Cicer arietinum L.) inoculated with plant growthpromoting rhizobacteria. Aust J Crop Sci 5(1):44–48

Rudresh D, Shivaprakash M, Prasad R. 2005. Effect of combined application of Rhizobium, phosphate solubilizing bacterium and Trichoderma spp. on growth, nutrient uptake and yield of Chickpea aritenium L. Applied Soil Ecology 28, 139-146. http://dx.doi.org/ 10.1016/j.apsoil.2004.07.005

Rugheim AME, Abdelgani ME. 2009. Effects of Rhizobium and Bacillus Megatherium var. Phosphaticum strains and chemical fertilizers on symbiotic properties and yield of faba bean (Vicia Faba L.). Advances in Environmental Biology 3, 337-346.

Ryan J, Estefan G, Rashid A. 2007. Soil and plant analysis laboratory manual, ICARDA.

Shah  Z,  Shah  S,  Peoples  M,  Schwenke  G, Herridge D. 2003. Crop residue and fertiliser N effects on nitrogen fixation and yields of legume– cereal rotations and soil organic fertility. Field Crops Research 83, 1-11.

Somasegaran P, Hoben HJ. 1985. Methods in legume-Rhizobium technology, University of Hawaii NifTAL Project and MIRCEN, Department of Agronomy and Soil Science, Hawaii Institute of Tropical Agriculture and Human Resources, College of Tropical Agriculture and Human Resources.

Steel RG, Torrie JH, Dickey DA. 1980. Principles and procedures of statistics: a biometrical approach. McGraw-Hill, New York, 168.

Turan M, Ataoğlu N, Şahιn F. 2006. Evaluation of the capacity of phosphate solubilizing bacteria and fungi on different forms of phosphorus in liquid culture. Journal of Sustainable Agriculture 28, 99-108.

Van Rhijn P, Fujishige NA, Lim PO, Hirsch AM. 2001. Sugar-binding activity of pea lectin enhances heterologous infection of transgenic alfalfa plants by Rhizobium leguminosarum biovar viciae. Plant physiology 126, 133-144.

Vance CP. 2001. Symbiotic nitrogen fixation and phosphorus acquisition. Plant nutrition in a world of declining renewable resources. Plant physiology 127, 390-397.

Vendrell PF, Zupancic J. 1990. Determination of soil nitrate by transnitration of salicylic acid. Communications in Soil Science and Plant Analysis 21, 1705-1713.

Wani P, Khan M, Zaidi A. 2007. Co-inoculation of nitrogen-fixing and phosphate-solubilizing bacteria to promote growth, yield and nutrient uptake in Chickpea. Acta Agronomica Hungarica 55, 315-323. DOI: 10.1556AAgr.55.2007.3.7

Yadegari M, Rahmani H, Noormohammadi G, Ayneband A. 2008. Evaluation of bean (Phaseolus vulgaris) seeds inoculation with Rhizobium phaseoli and plant growth promoting rhizobacteria on yield and yield components. Pak J Biol Sci 11, 1935-1939 http://dx.doi.org/10.1007/978-3642-1969-7.

Yu Y, Chu X, Pang G, Xiang Y, Fang H. 2009. Effects of repeated applications of fungicide carbendazim on its persistence and microbial community in soil. Journal of Environmental Sciences 21, 179-185.

Zahir A, Yasin H, Naveed M, Anjum M, Khalid M.  2010.  L-Tryptophan  application  enhances  the effectiveness of Rhizobium inoculation for improving growth and yield of mungbean (Vigna Radiata L. Wilczek). Pak J Bot 42, 1771-1780.

Zaidi A, Khan MS, Amil M. 2003. Interactive effect of rhizotrophic microorganisms on yield and nutrient uptake of chickpea, Cicer arietinum L. European Journal of Agronomy 19, 15-21. http://dx.doi.org/10.1016/ SI 161-0301(02)00015-1

Zia MS, Aslam M, Baig M, Ali A. 2000. Fertility issues and fertilizer management in rice–wheat system: A review. Quart. Sci. Vision 5, 59-73.