Effect of salinity stress on some physiological traits of spring rapeseed genotypes at seedling stage

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Research Paper 01/12/2016
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Effect of salinity stress on some physiological traits of spring rapeseed genotypes at seedling stage

Nima Dolatabadi, Mahmoud Toorchi, Mostafa Valizadeh, Ali Bandehagh
J. Bio. Env. Sci.9( 6), 135-142, December 2016.
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To study the effects of salinity on rapeseed (Brassica napus L.) and clear the role of K+ and Na+ in salt stress tolerance, an experiment was carried out in three salinity levels (0, 150 and 300 mM) and 16 genotypes with three replications under hydroponic culture system in greenhouse of university of Tabriz. Growth and physiological traits were measured 28 days after imposing NaCl stress at the end of seedling stage (42 days old).Salinity stress was affected all of the traits significantly. All of the traits were decreased by salt stress except electrolyte leakage and Na+ content of shoot and root. Significant differences were observed among genotypes in all of the studied traits, which indicate valuable genetic variability between the genotypes. Safi7 and Option500 genotypes with the highest and lowest performance in growth and physiological traits were identified as tolerant and susceptible genotypes, respectively. Safi7 genotype has the lowest Na+ content of shoot and highest shoot K+/Na+ ratio while genotype Option500 has the highest Na+ content of shoot and the lowest shoot K+/Na+ ratio. Due to the amount of K+ in shoots and roots weren’t significantly changed in genotypes; it seems rapeseed genotypes cope with salt stress by removing undesirable Na+ ions, not through adsorption of K+ ions.


Asghari A, Ansari MH, Shahbazi H. 2011. Evaluation of salt tolerance of rapeseed (Brassica napus L.) cultivars using physiological markers. Plant Ecophysiology 3, 1-4.

Ashraf M. 2004. Some important physiological selection criteria for salt tolerance in plants. Flora 376, 361-376.

Ashraf M, Akram NA. 2009. Improving salinity tolerance of plants through conventional breeding and genetic engineering: An analytical comparison. Biotechnology Advances 27, 744-752.

Ashraf M, McNeilly T. 2004. Salinity Tolerance in Brassica Oilseeds. Critical Reviews in Plant Sciences 23, 157-174.

Athar HR, Ashraf M, Wahid A, Jamil A. 2009. Inducing salt tolerance in canola (Brassica napus L.) by exogenous application of glycinebetaine and proline: Response at the initial growth stages. Pakistan Journal of Botany 41, 1311-1319.

Bandeh-hagh A, Toorchi M, Mohammadi A, Chaparzadeh N, Salekdeh GH, Kazemnia H. 2008. Growth and osmotic adjustment of canola genotypes in response to salinity. Journal of Food, Agriculture and Environment 6, 201-208.

Bandehagh A, Uliaie ED, Salekdeh GH. 2013. Proteomic analysis of rapeseed (Brassica napus L.) seedling roots under salt stress. Annals of Biological Research 4, 212-221.

Bennabi F, Belkhodja M, Boukraa D, Bouhadda Y, Salmi Z. 2013. The Attitude of a Saharan Variety Tadalaghte (Phaseolus vulgaris L.) Put under Stress of Salinity. Advanced Studies in Biology 5, 347-362.

Chinnusamy V, Zhu J, Zhu J-K. 2006. Salt stress signaling and mechanisms of plant salt tolerance. Genetic engineering 27, 141-177.

Dolatabadi N, Toorchi M, Shakiba M-R, Kazemnia H, Komatsu S. 2012. The response and protein pattern of spring rapeseed genotypes to sodium chloride stress. African Journal of Agricultural Research 7, 755-763.

Gul H, Ahmed R, Hamayun M, Qasim M. 2014. Growth Performance of Canola Grown Under Different Salinity Regimes. International Journal of Emerging Technology and Advanced Engineering 4, 59-68.

Gupta B, Huang B. 2014. Mechanism of salinity tolerance in plants: Physiological, biochemical and molecular characterization. International Journal of Genomics 2014.

Hajiaghaei Kamrani M, Hosseinniya H, Azam R chegeni. 2013. Effect of salinity on the growth characteristics Of canola (Brassica napus L.). Technical Journal of Engineering and Applied Sciences 3, 2327-2333.

Haq TU, Akhtar J, Ali A, Maqbool MM, Ibrahim M. 2014. Evaluating the response of some canola (Brassica napus L.) cultivars to salinity stress at seedling stage. Pak. J. Agri. Sci 51, 571-579.

Heidari A, Toorchi M, Bandehagh A, Shakiba M. 2011. Effect of NaCl Stress on Growth, Water Relations, Organic and Inorganic Osmolytes Accumulation in Sunflower (Helianthus annuus L.) Lines. Universal Journal of Environmental Research and Technology 1, 351-362.

Joseph B, Jini D, Sujatha S. 2010. Biological and physiological perspectives of specificity in abiotic salt stress response from various rice plants. Asian Journal of Agricultural Sciences 2, 99-105.

Kandil a a, Sharief a E, Abido W a E, Ibrahim MMO. 2012. Response of some canola cultivars (Brassica napus L.) to salinity stress and its effect on germination and seedling properties. Journal of Crop Science 3, 95-103.

De Lacerda CF, Cambraia J, Oliva MA, Ruiz HA. 2005. Changes in growth and in solute concentrations in sorghum leaves and roots during salt stress recovery. Environmental and Experimental Botany 54, 69-76.

Mer RK, Prajith PK, Pandya DH, Pandey AN. 2000. Effect of salts on germination of seeds and growth of young plants of Hordeum vulgare, Triticum aestivum, Cicer arietinum and Brassica juncea. Journal of Agronomy and Crop Science 185, 209-217.

Miyamoto S, Oster MF, Rostle CT, Lenn EG. 2012. Salt Tolerance of Oilseed Crops during Establishment. Journal of Arid Land Studies 22, 147-151.

Morant-Manceau A, Pradier E, Tremblin G. 2004. Osmotic adjustment, gas exchanges and chlorophyll fluorescence of a hexaploid triticale and its parental species under salt stress. Journal of plant physiology 161, 25-33.

Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Annual review of plant biology 59, 651-681.

Nayyar H. 2003. Accumulation of osmolytes and osmotic adjustment in water-stressed wheat (Triticum aestivum) and maize (Zea mays) as affected by calcium and its antagonists. Environmental and Experimental Botany 50, 253-264.

Parida AK, Das AB. 2005. Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental Safety 60, 324-349.

Purty RS, Kumar G, Singla-Pareek SL, Pareek A. 2008. Towards salinity tolerance in Brassica: An overview. Physiology and Molecular Biology of Plants 14, 39-49.

Sairam RK, Rao KV, Srivastava G. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science 163, 1037-1046.

Shirazi MU, Rajput MT, ANSARI R, Khan MA, TAHIR SS. 2011. Salt tolerance in Brassica species at early seedling stage. Sindh University Research Journal-SURJ (Science Series) 43, 203-208.

Toorchi M, Naderi R, Kanbar A, Shakiba MR. 2011. Response of spring canola cultivars to sodium chloride stress. Annals of Biological Research 2, 312–322.

Toorchi M, Naderi R, Shakiba MR, Habib K. 2010. Ion accumulation and water relations in canola (Brassica napus L.) cultivars under salinity. Journal of Food, Agriculture & Environment 8, 852–856.

Zhu JK. 2001. Plant salt tolerance. Trends in Plant Science 6, 66–71.