Effect of seed priming through gibberellic acid (GA3) and imidacloprid on bismarckia palm seed

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Effect of seed priming through gibberellic acid (GA3) and imidacloprid on bismarckia palm seed

Usman Shoukat Qureshi, Muhammad Shakeel, Dr. Khalid Mahmmod Qureshi, Saman Chughtai, Dr. Aamir Saleem, Ali Raza Mir, Azqa Azhar Qureshi
Int. J. Biosci.8( 4), 18-27, April 2016.
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Bismarckia nobilis is an oldest solitary silver blue fan palm of tropics and subtropics areas all through the world. A slow growing elegant ornamental, has currently a strong world demand as an outdoor plant in landscape industry from past twenty years. It is ideal for both domestic and commercial gardens as single specimen are also attractive and well suited for most of the residential landscape but seed germination is quiet slow and unequal. Thus the aim of present study is to assess the effect of seed priming method using different concentration of gibberellic acid (750mg/L, 1000mg/L, 1250mg/L) and imidacloprid (4g/kg, 6g/kg and 8g/kg) on bismarckia nobilis seed by breaking dormancy to get early germination. The following growth attributes like germination rate, germination percentage, plant height, leaf area, number of leaves and chlorophyll contents were studied in this experiment. The results demonstrate that priming among all the treatments of imidacloprid @ 6g/kg showed marked influence on plant height, leaf area, germination rate whereas gibberellic acid @ 1000 kg/L revealed maximum germination percentage but no effect on chlorophyll content was studied through these chemicals. Based on the results, imidacloprid being a cost effective chemical could be used as pre-treatment for inducing early germination in bismarckia nobilis seed as compared to gibberellic acid.


Ayyappath R, Polavarapu S, McGuir M. 2000. Effectiveness of Thiamethoxam coated spheres against blueberry maggot files (Diptera: Tephritidae). Journal of Economics Entomology 93, 1473-1479. http://dx.doi.org/10.1603/0022-0493-93.5.1473

Abou-Dahab AM, Shafiq Y, AL kinay A. 1975. Effect of gibberelic acid, B-nine and scarification on germination of seeds of Pistacia Khinyuh stock. Mesopotamia Journal of Agriculture 10(1-2), 13-19.

Albert R, Vogt. 1970. Effect of gibberelic acid on germination and initial seedling growth of northern red oak. Forest Science 16(4), 453-459.

Anon. 1976. International Rules for Seed Testing. International Seed Testing Association. Seed Science and Technology 4, 51-177.

Ballington JR. 1984. Greenhouse forcing to reduce the time between generations in blueberry (Vacciniumspp) breeding. Hortscience 19, 542.

Bai D, Lummis SCR, Leicht W, Breer H, Sattelle DB. 1991. Actions of imidacloprid and a related nitromethylene on cholinergic receptors of an identified  insect  motor  neurone.  Pesticide  Science 33(2), 197-204. http://dx.doi.org/10.1002/ps.2780330208.

Broschat TK, Donseman H. 1987. Effects of Fruit Maturity, Storage, Presoaking, and Seed Cleaning on Germination in Three Species of Palms. Journal of Environmental Horticulture 5(1), 6-9.

Buchholz A, Nauen R. 2002. Translocation and translaminar bioavailability of two neonicotinoid insecticides after foliar application to cabbage and cotton. Pest Management Science 58(1), 10-16. http://dx.doi.org/10.1002/ps.401

Bahrani A, Pourreza J. 2012. Gibberellin acid and salicylic acid effect on seed germination and seedling growth of wheat under salt stress condition. World Applied Sciences Journal 18(5), 633-641. http://dx.doi.org/10.5829/idosi.wasj.2012.18.05.137 2.

Baskin  CC,  Baskin  JM.  1998.  Seeds:  ecology, biogeography and evolution of dormancy and germination. San Diego: Academic Press. Annals of botany 86(3), 705-707. http://dx.doi.org/10.1006/anbo.2000.1238.

Chandra JP, Chauhan PS. 1976. Notes on germination of spruce seeds with gibberellic acid. The Indian Forester 102(1), 721-725.

Cataneo AC, Ferreira LC, Carvalho JC, Andréo-Souza Y, Corniani N, Mischan MM, Nunes JC. 2010. Improve germination of soybean seed treated with thiamethoxam under drought conditions. Seed science and technology 38(1), 248-251. http://dx.doi.org/10.15258/sst.2010.38.1.27

Chin HF, Krishnapillay B, Alang ZC. 1988. Breaking dormancy in Kentia palm seeds by infusion technique. Pertanika 11(1), 137-141.

Delgarde S, Rouland-Lefevre C. 2002. Evaluation of the effects of Thiamethoxam on three species of African termite (Isoptera: Termitidae) Crop Pests. Journal of Economic Entomology 95(3), 531- 536. http://dx.doi.org/10.1603/0022-0493-95.3.531

Dewir YH, El-Mahrouk ME, Naidoo Y. 2011. Effects of some mechanical and chemical treatments on seed germination of Sabal palmetto and Thrinax morrisii palms. Australian Journal of Crop Science 5(3), 248-253.

Dweikat IM, Lyrene PM. 1988. Response of high bush blueberry seed germination to gibberellin A3 and 6N-benzy-ladenine. Canadian Journal of Botany 67(11), 3391–3393. http://dx.doi.org/10.1139/b89-412

Farmer RE. 1997. Seed Ecophysiology of Temperate and Boreal Forest Trees. St Lucie Press Delray Beach Florida, 253 p.

Farooq M, Basra SMA, Khan MB. 2007. Seed priming improves growth of nursery seedlings and yield of transplanted rice. Archives of Agronomy and Soil Science 53(3), 315-326. http://dx.doi.org/10.1080/03650340701226166

Foster SP, Denholm I, Thompson R. 2003. Variation in response toneonicotenoids insecticides in peach-potato aphids, Myzus persicae (Hemiptera: Aphididae). Pest Management Science 59(2), 166-173. http://dx.doi.org/10.1002/ps.570.

Finch-Savage WE, Leubner-Metzger G. 2006. Seed dormancy and the control of germination. New Phytologist 171(3), 501-523. http://dx.doi.org/10.1111/j.1469-8137.2006.01787.x.

Giba Z, Grubisic D, Konjevic R. 1993. The effect of white light, growth regulators and temperature on the germination of blueberry (Vaccinium myrtillus L.) seeds. Seed Science and Technology 21(3), 521-529.

Gilman E, Watson D. 2011. Bismarckia nobilis: Bismarck Palm. ENH260. UF/IFAS, Gainesville, Florida.

Gilman EF. 1997. Tress of urban and suburban landscape. Delmar publisher, p170.

Gomes PB, Valio IFM, Martins FR. 2006. Germination of Geonoma brevispatha (Arecaceae) in laboratory and its relation to the palm spatial distribution in a swamp forest. Aquatic Botany 85(1),16-20. http://dx.doi.org/10.1016/j.aquabot.2006.01.008.

Gupta R, Chakrabarty SK. 2013. Gibberellic acid in plant. Plant signal behavior 8(9), e25504. http://dx.doi.org/10.4161/psb.25504

Herrera J, Alizaga R, Guevara E. 1998. Use of chemical treatments to induce seed germination in oil palm Elaeis guineensis Jacq. ASD Oil Palm Papers 18, 1-16.

Hartmann HT, Kester DL. 1975. Plant Propagation, Principles and  Practices Prentice-Hall Inc. New Jersey.

Horii A, McCue P, Shetty K. 2007. Enhancement of seed  vigour  following  insecticide  and  phenolics elicitor treatment. Bioresource. Technology 98(3), 623-632. http://dx.doi.org/10.1016/j.biortech.2006.02.028

Hussain I, Ahmad R, Farooq M, Rehman A, Amin M, Bakar MA. 2014. Seed priming: a Tool to invigorate the seeds. Scientia Agriculturae 7(3), 122-128. http://dx.doi.org/10.15192/PSCP.SA.2014.3.3.122128

Jensen M, Eriksen EN. 2001. Development of primary dormancy in seeds of Prunus avium during maturation. Seed Science and Technology 29, 307– 320.

Jones DL. 1984. Palms in Australia. Reed Books Pty Ltd. pp278.

Karam NS, Al-Salem MM. 2001. Breaking dormancy in Arbutus andrachna L. seeds by stratification and gibberellic acid. Seed Science and Technology 29(1), 51– 56.

Klinger R, Rejmanek M. 2010. A strong conditional mutualism limits and enhances seed dispersal and germination of a tropical palm. Oecologia 162(4), 951-963. http://dx.doi.org/10.1007/s00442-009-1542-3.

Kerbauy GB, 2004. Fisiologia Vegetal. Rio de Janeiro, Editora Guanabara Koogan, p. 452.

Krishnamoorthy HN. 1973. Gibberellins and plant growth. Wiley Eastern Limited New Delhi 356, 19-114.

Laurent FM, Rathahao E. 2003. Distribution of [14C] Imidacloprid in Sunflowers (Helianthus annuus L.) following Seed Treatment. Journal of Agricultural and Food Chemistry 51(27), 8005-8010. http://dx.doi.org/10.1021/jf034310n

Linkies A, Graeber K, Knight C, Leubner-metzger G. 2010. The evolution of seeds. New Phytologist 186(4), 817-831.

McDonald MB. 2000. Seed priming. Black M, Bewley JD (eds), seed technology and its biological basis. Sheffield Academic Press Ltd Sheffield UK, 287-325.

Matsuda K, Shimomura M, Kondo Y, Ihara M, Hashigami K, Yoshida N, Raymond V, Mongan NP, Freeman JC, Komai K, Sattelle DB. 2000. Role of loop D of the α7 nicotinic acetylcholine receptor in its interaction with the insecticide imidacloprid and related neonicotinoids. British Journal of Pharmacy 130(5), 981-986. http://dx.doi.org/10.1038/sj.bjp.0703374

Moussa H, Margolis HA, Dube P, Odongo J.1998. Factor affecting the germination of doum palm(Hyphaene thebaica Mart.) seeds from the semi-arid zone of Niger, West Africa. Forest Ecology and Management 104(1-3), 27-41. http://dx.doi.org/10.1016/S0378-1127(97)00230-2

Muller J. 1991. Determining leaf surface area by means of linear measurements in wheat and Triticale (brief report). Archiv Fuchtungsforsch 21, 121–123.

Nagao MA, Kanegawa K, Sakai WS. 1980. Accelerating palm seed germination with Gibberellic acid, scarification, and bottom heat. Hortscience 15, 200-201.

Nauen R, Tollo B, Tietjen K, Elbert A. 1998. Antifeedant effect, biological efficacy and high affinity binding of imidacloprid to acetylcholine receptors in Myzus Persicae and Myzus nicotianae. Pesticide Science 51, 52-56. http://dx.doi.org/10.1002/(SICI)10969063(199806)53:2<133::AID-PS756>3.0.CO;2-D.

Naeem MA, Muhammud S. 2006. Effect of seed priming on growth of barley (Hordeum vulgare) by using brackish water in salt affected soils. Pakistan Journal of Botany 38(3), 613-622.

Orozco Segovia A, Batis AI, Rojaaréchiga M, Mendoza A. 2003. Seed biology of palms: a review. Palms 47(2), 79-94.

Olveria TGS, Junior AGR, Pereia-de-Souza P, Ribeiro LM. 2013. Use of phytoregulators in overcoming macaw palm seed dormancy. Acta Scientiarum Agronomy 35(4), 505-511. http://dx.doi.org/10.4025/actasciagron.v35i4.16385.

Potvin C, Cansari R, Hutton J, Caisamo I, Pacheco B. 2003. Preparation for propagation: understanding germination of giwa (Astrocaryum standleyanum) wagara (Sabal mauritiiformis) and eba (Socratea exorrhiza) for future cultivation. Biodiversity Conservation 12(11), 2161-2171. http://dx.doi.org/10.1023/A:1024511727478

Pinheiro CUB. 2001. Germination strategies of palms: the case of Schippia concolor Burret in Belize. Brittonia 53(4), 519-527. http://dx.doi.org/10.1007/BF02809652

Roberto GG, Habermann G. 2010. Morphological and physiological responses of the recalcitrant Euterpe edulis seeds to light, temperature and gibberellins. Seed Science and Technology 38(2), 367-378.

Rood SB, Buzzell RI, Major DJ, Pharis RP. 1990. Gibberellins and heterosis in maize: quantitative relationship. Crop Science 30(2), 281– 286. http://dx.doi.org/10.2135/cropsci1990.0011183X003 000020008x

Ribeiro LM, Garcia QS, Oliveira DMT, Neves SC. 2010. Critérios para o teste de tetrazólio na estimative do potencial germinativo em macaúba. Pesquisa Agropecuária Brasileira, 45(4), 361-368.

Schulz T, Thelen K, Difonozo C. 2007. Neonicotinoid seed treatment for soya bean. Michigan State of University, department of crop and soil science. Michigan State University Extension, Department of Entomology.

Schmuck R, Nauen R, Ebbinghaus-Kintscher U. 2003. Effects of imidacloprid and common plant metabolites of imidacloprid in the honeybee: toxicological and biochemical considerations. Bulletin of Insectology 56(1), 27-34.

Sur R, Strok A. 2003. Uptake, translocation and metabolism of imidaclorpid in plants. Bulletin of insectology 56(1), 35-40.

Singh H, Dara BL. 1971. Influence of presoaking of seeds with gibberellin and auxins on growth and yield attributes of wheat (Triticum aestivum L.) under high salinity, sodium adsorption ratio and boron levels. The Indian Journal of Agricultural Sciences 41, 998-1103.

Venator  CR.  1972.  Effect  of  gibberellic  acid  on Forest Science. 18(4), 331.germination  of  low  vigour  Honduras  Pine  Seeds.

Wagner  RI. 1982.  Raising ornamental  palms. Principes 26(2), 86-102.

Yamamoto I, Yabuta G, Tomizawa M, Saito T, Miyamoto   T, Kagabu   S. 1995.   Molecular mechanism for selective toxicity of nicotinoids and neonicotinoids Journal of Pesticide Science 20(1), 33-40. http://dx.doi.org/10.1584/jpestics.20.33

Yang QH, Ye WH, Yin XJ. 2007. Dormancy and germination   of   Areca   triandra   seeds.   Scientia Horticulturae 113(1), 107-111. http://dx.doi.org/10.1016/j.scienta.2007.01.028