Welcome to International Network for Natural Sciences | INNSpub

Effect of selected pesticidal plants leaf powder on Callosobrunchus maculatus in stored cowpea (Vigna unguiculata (L.) Walp

Research Paper | June 1, 2020

| Download 23

Juliana Godifrey, Ernest Mbega, Patrick A. Ndakidemi

Key Words:

Int. J. Biosci.16( 6), 1-10, June 2020

DOI: http://dx.doi.org/10.12692/ijb/16.6.1-10


IJB 2020 [Generate Certificate]


Among the major insect storage pests of cowpea are cowpea weevils (Callosobruchus maculatus) which is a big threat causing huge postharvest loss.  This study used double bagging experiment to test the pesticidal activity of plants leaf powders of Ocimum suave, Hyptis suaveolens and Dysphania ambrosioides on Callosobrunchus maculatus in stored cowpea. The experiment was arranged in Randomized Block Design (RBD) with 3 replications. The plant powders were applied at the rates of 30, 60 and 90 g 1.5 kg-1 of cowpea seeds spread on the surface of the inner cotton bag of 2 kg filled with 1.5 kg of cowpea seeds. The storage time was three months consecutive. The effectiveness was compared with the synthetic pesticide Actelic® dust (2 g) as the positive control and untreated cowpea seeds as a negative control. Based on the results, all pesticidal leaf powder showed a different level of pesticidal activities at the tested rates. Higher dosages of plant leaf powders at 60 and 90 g for H. suaveolens and D. ambrosioides and 90 g for O. suave significantly (P < 0.001) affected insects’ mortality, survivorship and reduced seed damage. Comparatively, H. suaveolens and D. ambrosioides at 90 g were more effective in inhibiting egg deposition by C. maculatus just as successful as the positive control. Therefore, this study demonstrates that, O. suave, H. suaveolens and D. ambrosioides leaf powder can effectively offer protection in stored cowpea seeds against C. maculatus when applied at high dosages.


Copyright © 2020
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Effect of selected pesticidal plants leaf powder on Callosobrunchus maculatus in stored cowpea (Vigna unguiculata (L.) Walp

Adebayo RA, Eyo ME. 2014. Evaluation of the insecticidal effects of Hyptis suaveolens (L.) for the management of Callosobruchus maculatus (F.) on two varieties of cowpea, Vigna unguiculata walp. International Journal of Horticulture 17(4), 1- 6.

Adedire CO, Obembe OM, Akinkurolere RO, Oduleye SO. 2011. Response of Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae) to extracts of Cashew kernels., Journal of plant diseases and protection 2(118), 75-79.

Asawalam EF, Emosairue SO, Ekeleme F, Wokocha RC. 2006. Insecticidal effects of powdered parts of Eight Nigerian plant species against maize weevil Sitophilus zeamais motschulsk (Coleaoptera: Curculionidae). Nigeria Agricultural Journal 1(37), 106-116.

Bekele AJ, Obengofori D, Hassanali A. 1996. Evaluation of Ocimum suave (Willd) as a source of repellents, toxicants and protectants in storage against three stored product insect pests. International Journal of Pest Management 2(42), 139–142.

Brisibe EA, Adugbo SE, Ekanem U, Brisibe F, Figueira GM. 2011. Controlling bruchid pests of stored cowpea seeds with dried leaves of Artemisia annua and two other common botanicals. African Journal of Biotechnology 47(10), 9593-9599.

Chiasson H, Vincent C, Bostanian N. 2004. Insecticidal properties of a Chenopodium-based botanical. Journal of Economic Entomology 4 (97), 1378–1383.

Chi MV, Apiah SP. 2012. Toxic and feeding deterrent effects of Hyptis suaveolens and Hyptis spicigera extracts on cowpea weevils (Callosobruchus maculatus). Canadian Journal of Pure and Applied sciences 6, 1967–1972.

Chu SS, Feng Hu J, Liu ZL. 2011. Composition of essential oil of Chinese Chenopodium ambrosioides and insecticidal activity against maize weevil, Sitophilus zeamais. Pest Management Science 6(67), 714-718.

Dembitsky V, Shkrob I, Hanus LO. 2008. Ascaridole and related peroxides from the genus Chenopodium. Biomedical Papers of the Medical Faculty of Palacky University in Olomouc 2(152), 209–215.

Denloye A, Makanjuola W, Teslim O, Alafia O, Kasali A, Eshilokun A. 2010. Toxicity of Chenopodium ambrosioides L. (Chenopodiaceae) products from Nigeria against three storage insects. Journal of Plant Protection Research 3(50), 379–384.

Hasan K, Naser AA, Sabiha S, Nesa M, Khan M, Islam N. 2018. Control potentials of Hyptis suaveolens L. (Poit.) extracts against Artemia salina L. Nauplii and Tribolium castaneum (HBST.) adults. Journal of Entomology and Zoology Studies 6(1), 785-789.

Hassanali A, Lwande W, Ole-Sitayo N, Moreka L, Nokoe S. Chapya A. 1990. Weevil repellent constituents of Ocimum suave leaves and Eugenia caryophyllata cloves used as grain protectant in parts of Eastern Africa. Discovery and Innovations 2, 91-95.

Ileke KD, Olotuah OF. 2012. Bioactivity of Anacardium occidentale (L) and Allium sativum (L) powders and oils extracts against cowpea Bruchid. Callosobruchus maculatus (Fab.) [Coleoptera: Chrysomelidae], International Journal of Biology 1(4), 96-103.

Ileke KD, Bulus DS, Aladegoroye AY. 2013. Effects of three medicinal plant products on survival, oviposition and progeny development of cowpea bruchid, Callosobruchus maculatus (Fab.) [Coleoptera: Chrysomelidae] infesting cowpea seeds in storage. Jordan Journal of Biological Science 4(5), 61-66.

Mkenda PA, Stevenson P C, Ndakidemi P, Farman D, Belmain SR. 2015. Contact and fumigant toxicity of five pesticidal plants against Callosobruchus maculatus (Coleoptera: Chrysomelidae) in stored cowpea (Vigna unguiculata). International Journal of Tropical Insect Science 4(35), 172-184.

Myaka F, Kabissa J, Myaka D, Mligo J. 2002. Farmer participatory evaluation of newly developed components of cowpea and cotton intercropping technology, challenges and opportunities for enhancing sustainable cowpea production. In Proceedings of the World Cowpea Conference III held at the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria 329–337.

Muzemu S, Chitamba J, Mutetwa B. 2013. Evaluation of Eucalyptus tereticornis, Tagetes minuta and Carica papaya as stored maize grain protectants against Sitophilus zeamais (Motsch.) (Coleoptera: Curculionidae). Agriculture, Forest and Fisheries 2(5), 196-201.

Obeng-Ofori D, Reichmuth CH. 1997. Bioactivity of Eugenol, a major component of essential oil of Ocimum suave (Wild.) against four species of stored-product Coleoptera. International Journal of Pest Management 43(1), 89-94.

Obeng-Ofori D, Jembere B, Hassanali A, Reichmuth C. 2000. Effectiveness of plant oils and essential oil of Ocimum plant species for protection of stored grains against damage by stored product beetles. In Proceedings of the 7th International Working Conference on Stored-product Protection. Beijing–China  799-808.

Odeyemi OO, Gbaye OA, Akeju O. 2006. Resistance of Callosobruchus maculatus (Fab.) to Pirimiphos methyl in three zones in Nigeria. In Proc. 9th Int. Work. Conf. Stored-Prod. Protect. Campinas, Sauo Paulo, Brazil 1518, 324-329.

Ojianwuna CC, Umoru PA. 2010. Acute toxicity of lemon grass (Cymbopogon Citratus) and wild basil (Ocimum Suave) applied as mixed and individual powders against the cowpea bruchids, Callosobruchus maculatus, in cowpea. African Journal of Agricultural Research 6, 6311-6319.

Salimena JP, Monteiro FP, de Souza PE, de Souza JT. 2015. Extraction of essential oil from inflorescences of Dysphania ambrosioides and its activity against Botrytis cinerea. Journal of Medicinal Plants Research 39(9), 1006-1012.

Sharma N, Tripathi A. 2008. Integrated management of postharvest Fusarium rot of gladiolus corms using hot water, UV-C and Hyptis suaveolens (L.) Poit. essential oil. Postharvest biology and technology 2(47), 246-254.

Smith AH, Liburd OE. 2012. Intercropping crop diversity and pest management. University of Florida, IFAS Extension, ENY 862 1-7.

Tapondjou L, Adler C, Bouda H, Fontem D. 2002. Efficacy of powder and essential oil from Chenopodium ambrosioides leaves as post-harvest grain protectants against six-stored product beetles. Journal of Stored Products Research 38(2002), 395–402.

Tripathi AK, Upadhyay S. 2009. Repellent and insecticidal activities of Hyptis suaveolens (Lamiaceae) leaf essential oil against four stored-grain Coleopteran pests. International Journal of Tropical Insect Science 4(29), 219-28.