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Effect of sodium hypochlorite on in vitro sterilization of six promising potato cultivars

Research Paper | August 1, 2017

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Abdul Ahad Qureshi, Nadeem Akhtar Abbasi, Ishfaq Ahmad Hafiz, Muhammad Kausar Nawaz Shah

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Int. J. Biosci.11( 2), 49-57, August 2017

DOI: http://dx.doi.org/10.12692/ijb/11.2.49-57


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In vitro establishment of pathogen free, healthy germplasm for commercial and research purpose need asepsis. Each plant and type of explant has different protocol for disinfection. Excess amount of these sterilant can cause toxic affect. Therefore, this study was conducted to investigate the effect of different concentrations (0, 5, 10 and 15 %) and time duration (5, 10 and 15 minutes) of Sodium hypochlorite (NaOCl) on in vitro sterilization of potato explant to find out best sterilant dose with least toxic effect on in vitro culture establishment. The study shows that 10% NaOCl for 15 minutes proved significant sterilant while keeping low toxicity. It gave maximum number of healthy plants in each variety as well as minimum quantity of infected and non-growing culture with 92.2% healthy, 3.3% infected and 4.5% non-growing cultures for Kuroda, 91% healthy, 4.5% infected and 4.5% non-growing cultures for Astrex, 87.7% healthy, 5.6% infected and 6.7% non-growing cultures for Lady Rosetta, 92.2% healthy, 4.5% infected and 3.3% non-growing cultures for Sante, 94.5% healthy, 3.3% infected and 2.2% non-growing cultures for Diamant and 93.3% healthy, 4.5% infected and 2.2% non-growing cultures for Hermes. While higher doses eliminate the contaminants, but endure toxic effects on explant.


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