Effect of Solvents on the biological activities and phenolic composition of Achyranthes aspera
Paper Details
Effect of Solvents on the biological activities and phenolic composition of Achyranthes aspera
Abstract
Plants kingdom have a variety of species which are being used for the treatment of various disease in different parts of world including Asia. Synthetic compounds used in the treatment of have side effects and more costly. So it is need to find such compounds from natural sources which have less side effect and economical. So the present research work was focus on the evaluation of Achyranthes aspera regarding its antioxidant and antimicrobial potential by extracting in various solvents such as methanol, ethanol and aqueous. Aqueous solvent showed highest extraction yield 10.3% than other solvent. Antibacterial activity was performed by well diffusion assay. Aqueous extract showed maximum antibacterial (29mm and 23mm) activity against both S. aureous and Escherichia coli respectively by disk inhibition assay. While other both extracts also showed significant activity. Antioxidant activity was performed through 1, 1-diphenyl-2-picrylhydrazyl scavenging free radical assay which showed that all extracts showed significant (p<0.05) activity. Methanolic extract showed highest antioxidant activity (68.33%) followed by aqueous extract (53.18%), while ethanolic extract showed lowest antioxidant activity (39.47%). Total phenolic and flavonoid contents were determined by spectrophotometer assay which showed that aqueous extract contain highest phenolic and flavonoid contents as 230 mg/g equ G.A and 137 mg/q equ QE. The characterization of phenolic compounds was done by high performance liquid chromatography which revealed the presence of chromatotropic acid, quercitin, gallic acid, syringic acid, ferulic acid, trans 4-hydroxy-3-methoxy cinamic acid and vinillic acid are the major phenolic in all extracts.
Al-Jaber NA, Awaad AS, Moses JE. 2011. Review on some antioxidant plants growing in Arab world. Journal of Saudi Chemical Society, 15, 293-307. http://dx.doi.org/10.1016/j.jscs.2011.07.004
Archivio DM, Filesi C, Di Benedetto R, Gargiulo R, Giovannini C, Masella R. 2007. Polyphenols, dietary sources and bioavailability. Annali-IstitutoSuperiore di Sanita, 43, 348-352.
Bakkali F, Averbeck S, Averbeck D, Idaomar M. 2008. Biological effects of essential oils–a review. Food and chemical toxicology, 46, 446-475. http://dx.doi.org/10.1016/j.fct.2007.09.106
Bamane FH, Badr JM, Amin OARM. 2012. Antioxidant activities and flavonoid contents of selected plants belonging to Family Loranthaceae. African Journal of Biotechnology, 11, 14380-14385. http://dx.doi.org/10.5897/AJB12.2093
Bernhoft A. 2010. A brief review on bioactive compounds in plants. Bioactive compounds in plants-benefits and risks for man and animals.
Brewer MS. 2011. Natural antioxidants: sources, compounds, mechanisms of action, and potential applications. Comprehensive Reviews in Food Science and Food Safety 10, 221-247.http://dx.doi.org/10.1111/j.15414337.2011.00156.x
Chaovanalikit A, Wrolstad RE. 2004. Anthocyanin and polyphenolic composition of fresh and processed cherries. Journal of Food Science, 69, FCT73-FCT83. http://dx.doi.org/10.1111/j.1365-2621.2004.tb17859.x
Fang YZ, Yang S, Wu G. 2002. Free radicals, antioxidants, and nutrition. Nutrition, 18, 872-879. http://dx.doi.org/10.1016/S08999007(02)00916-4
Fernández-Bolaños J, Rodríguez G, Gómez E, Guillén R, Jiménez A, Heredia A, Rodríguez R. 2004. Total recovery of the waste of two-phase olive oil processing: isolation of added-value compounds. Journal of agricultural and food chemistry 52, 5849-5855. http://dx.doi.org/10.1021/jf030821y
Guerrero-Rodríguez E, Solís-Gaona S, Hernández-Castillo FD, Flores-Olivas A, Sandoval-López V, Jasso-Cantú D. 2007. Actividad Biológica in vitro de Extractos de Flourensiacernua DC en Patógenos de Postcosecha: Alternaria alternata (Fr.) Keissl., Colletotrichum gloeosporioides (Penz.) Penz. Sacc., Penicillium digitatum (Pers.: Fr.) Sacc. Revista mexicana de Fitopatología 25, 48-53.
Harasym J, Oledzki R. 2014. Effect of fruit and vegetable antioxidants on total antioxidant capacity of blood plasma. Nutrition 30, 511-517. http://dx.doi.org/10.1016/j.nut.2013.08.019
Huang D, Ou B, Prior RL. 2005. The chemistry behind antioxidant capacity assays. Journal of agricultural and food chemistry, 53, 1841-1856. http://dx.doi.org/10.1021/jf030723c
Kähkönen MP, Hopia AI, Heinonen M. 2001. Berry phenolics and their antioxidant activity. Journal of Agricultural and Food Chemistry,49, 4076-4082. http://dx.doi.org/10.1021/jf010152t
La Marca M, Beffy P, Della Croce C, Gervasi PG, Iori R, Puccinelli E, Longo V. 2012. Structural influence of isothiocyanates on expression of cytochrome P450, phase II enzymes, and activation of Nrf2 in primary rat hepatocytes. Food and chemical toxicology 50, 2822-2830. http://dx.doi.org/10.1016/j.fct.2012.05.044
Mohamed A A, Sami IA, El-Baz FK. 2013. Antioxidant and Antibacterial Activities of Crude Extracts and Essential Oils of Syzygium cumini Leaves. PLoSONE 8, 6026-6029. http://dx.doi.org/10.1371/journal.pone.0060269
Ningsih IY, Zulaikhah S, Hidayat MA, Kuswandi B. 2016. Antioxidant Activity of Various Kenitu (Chrysophyllum cainito L.) Leaves Extracts from Jember, Indonesia. Agriculture and Agricultural Science Procedia 9, 378-385. http://dx.doi.org/10.1016/j.aaspro.2016.02.153
Popa VI, Agache C, Beleca C, Popa M. 2002. Polyphenols from spruce bark as plant growth regulator. Crop Research-Hisar 24, 398-406.
Qayyum A, Sarfraz RA, Ashraf A, Adil S. 2016. Phenolic composition and biological (anti diabetic and antioxidant) activities of different solvent extracts of an endemic plant (Heliotropium strigosum). Journal of the Chilean Chemical Society, 61, 2828-2831. http://dx.doi.org/10.4067/S071797072016000100016
Queiroz YS, Ishimoto EY, Bastos DHM, Sampaio GR, Torres, EAFS. 2009. Garlic (Allium sativum L.) and ready-to-eat garlic products: In vitro antioxidant activity. Food Chemistry, 115, 371–374. http://dx.doi.org/10.1016/j.foodchem.2008.11.105
Singh HP, Mittal S, Kaur S, Batish DR, Kohli RK. 2009. Characterization and antioxidant activity of essential oils from fresh and decaying leaves of Eucalyptus tereticornis. Journal of agricultural and food chemistry 57, 6962-6966. http://dx.doi.org/10.1021/jf9012407
Tomás-Barberán FA, Robins RJ. 1997. Phytochemistry of fruit and vegetables. In International Symposium of Phytochemistry of Fruit and Vegetables (1995: Murcia, Spain). Clarendon Press; Oxford University Press.
Urquiaga INES, Leighton F. 2000. Plant polyphenol antioxidants and oxidative stress. Biological research, 33, 55-64. http://dx.doi.org/10.4067/S071697602000000200004
Yamamoto YY. 2000. Free radicals in chemistry, biology and medicine. OICA International (UK).
Yildirim AB, Karakas FP, Turker AU. 2012. In vitro antibacterial and antitumor activities of some medicinal plant extracts, growing in Turkey. Asian Pacific Journal of Tropical Medicine 6, 616-624
Muhammad Javid, Raja Adil Sarfraz, Faisal Nawaz, Abdul Qayyum, Abdur Rehman, Shahid Aziz, Syed Muhammad Imran Bin Riaz (2016), Effect of Solvents on the biological activities and phenolic composition of Achyranthes aspera; IJB, V9, N6, December, P145-153
https://innspub.net/effect-of-solvents-on-the-biological-activities-and-phenolic-composition-of-achyranthes-aspera/
Copyright © 2016
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0