Effect of the silver nanoparticles on the histology of albino lactating mice ovaries

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Research Paper 01/08/2018
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Effect of the silver nanoparticles on the histology of albino lactating mice ovaries

Noor Mahdi, Gazwa D. Al-Nakeeb
J. Bio. Env. Sci.13( 2), 68-75, August 2018.
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There is increasing interest and need to develop a deeper understanding of the nature, fate and behavior of nanoparticles in the environment. This is driven by the increased use of nanoparticles and the increased pressure to commercialize this growing technology. Silver nanoparticles (Ag NPs) are the most commonly nanoparticles used in various areas of research, because of their characteristic physical as: magnatic, mechanical: performance as well as antimicrobial effects as antiviral, antibacterial , so that exposure human to increase levels of nanoparticles. Therefore, this study aimed to investigate the histological effects of silver nanoparticles exposure on the ovary of the lactating albino mice. Sixty adult lactating female albino mice were divided into three main groups of (15) females exposed to 1p.p.m., (15) females were exposed by 1.5p.p.m. , (15) females were exposed to 2p.p.m. of silver nanoparticles solution for 7, 14  and 21 days as well as 3 control groups each group 5 mice contain by intra-peritoneal injection.  After the end of injection periods the samples of animals were sacrificed and dissected to remove the ovaries and kept in Bouins fluids for microscopic examination treated groups exhibited  different histopathological changes ,depending on the concentration of silver nanoparticles as : hyaline degeneration, hydropic degeneration, fibrous necrosis, and  on the period of exposure, these changes include shrinkage of Oocyte, fatty degeneration, amyloid protein precipitation, pyknosis , necrosis ,cloudy degeneration, fusion and swelling cells, Caceous necrosis different damage  effects  in silver accumulations was noted in the  ovary, with accumulations being significantly higher in female lactating mice, especially in the  cortex, follicles, stroma.


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