Paper Details

Effect of total flavonoid extract of Tanacetum parthenium L. (Feverfew) pollen grains on immune system responses in Balb/C mice

Masoomeh Jannesar, Ahmad Majd, Maryam Sharif Shoushtari, Mona Oraei

DOI: https://dx.dpi.org/10.12692/ijb/5.12.72-78

Int. J. Biosci. 5(12), 72-78. December, 2014. (PDF)

Abstract:

Tanacetum parthenium L. has been used in  traditional medicine especially for reducing fever and as an insect repellant. This plant has some pharmacological properties, such as anticancer, anti- inflammatory, and it’s also cardiotonic, antispasmodic and has emmenagogue properties and it can also be used as an enema for worms. In this study total flavonoid extract of T.parthenium pollen grains was evaluated for Immunomodulatory activity in male Balb/C mice by two methods: 1. Delayed type hypersensitivity (DTH) test, 2. Lymphocyte proliferation assay by the method MTT ((3-4, 5-dimethyl tetrazolyl-2) 2, 5 diphenyl) tetrazolyum bromide). Forty five Balb/C mice were classified in nine groups and treated with eight doses (1, 5, 10, 20, 30, 50, 70 and 100 mg/Kg) of total flavonoid extract. DTH response was significantly (P<0.01) increased at doses of 20, 30, 50 and 70 mg/Kg. The highest DTH response was observed at doses of 50 and 70 mg/Kg of the extract.  A significant increase (P<0.01) in lymphocyte immune response was observed at all doses of the extract but the best response was found at the dose of 30 mg/Kg of extract.  The results obtained in this study indicated that total flavonoid extract of T. parthenium pollen grains has significant immunomodulatory activity.