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Research Paper | April 1, 2014

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Effect of zinc and lead toxicity on some physiological parameters of Glycine max L.

Fatemeh Naghavi

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J. Bio. Env. Sci.4(4), 192-201, April 2014


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The presence of heavy metals is counted as one of the most important environmental stresses. In this research, the effects of different treatments of two heavy metals, Lead and Zinc on the growth and rate of Chlorophyll, sugar and protein are studied. The effects of different concentrations of ZnCl2 (5, 10, 15, 20 mM ) and PbCl2 ( 0.5, 2.5, 4.5, 6.5, mM ) on Soybean were examined. For this purpose, the seeds were transferred to flowerpots containing Loam – Clay soil after surface sterile. In each flowerpot were cultivated 5 seeds. For each treatment are considered 4 repeat. The duration of treatment period has continued for 14 days. The results showed that the stress of Pb caused the decrease in biomass weight. The stress of Zn terminated in decrease of biomass weight in all concentrations. The rate of Chlorophyll a, b and total Chlorophyll in comparison with control plant showed significant decrease in both treatments. The increase in sugar content except for the concentration of 0.5 mM Pb, was significant in comparison with control plant in other concentrations but, in the stress of Zn, this increase had significant effect in all concentrations. The containing of protein in plants under treatment with Zn and Pb had significant increase in all concentrations. The obtained results of this research indicated that in comparison with Zn, Soybean has more tolerance to Pb. It seems that in short term stress of Zn and Pb , the plant decreases stress intensity with induction of fast response, but, in spite of definitions that exist for the tolerable plants of Zn and Pb, it does not seem to include Soybean from tolerable plants to these heavy metals.


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Effect of zinc and lead toxicity on some physiological parameters of Glycine max L.

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