Effectiveness of Piper betel leaf extracts against Acinetobacter species isolated from bronchitis and pharyngitis patients

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Research Paper 01/11/2020
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Effectiveness of Piper betel leaf extracts against Acinetobacter species isolated from bronchitis and pharyngitis patients

Sehr Syed, Umair Azmat, Shaista Ilyas, Arslan Fazal, Tanzeela Murmim, Fakhra Riaz, Maryam Khan, Saba Shamim
Int. J. Biosci.17( 5), 162-175, November 2020.
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Acinetobacter species is associated with healthcare-associated infections especially chronic cough and other related complications. They are becoming increasingly antibiotic resistant. In the present work, the antimicrobial activity of different extract (water, ethanol, methanol, and chloroform) of Piper betel leaves were tested against Acinetobacter sp. SZ-1 and Acinetobacter baumanii TM-1 isolated from clinical samples collected from the patients suffering from pharyngitis, bronchitis and sore throat. For the antibacterial activity, different plant extracts were used in which Piper betel leaf stand out to be the most effective. Six solvents were used for this study in which only chloroformic extract showed the zones of inhibition. TLC analysis showed five spots with Rf 0.896,0.973,0.747,0.574 and 0.482. SDS-PAGE revealed proteins of different molecular weight when the bacterial cells were treated with ethanolic extract. It was also confirmed by Bradford analysis. GCMS analysis depicted different bioactive compounds including phytol; phenol,2,2-methylenebis[6-(1,1-dimethylethyl)-4-methyl; spirost-8-en-11-one,3-hydroxy-,(3β,5α,14β,20β,22β,25R) and 2,2,4-trimethyle-3-(3,8,12,16-tetramethyl-heptadeca-3,7,11,15-tetraenyl)-cyclohexanol. Furthermore, in silico analysis can enhance the existing knowledge about establishing the significance of P. betel leaves as an effective drug to treat the acute and chronic upper respiratory tract infections.


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