Welcome to International Network for Natural Sciences | INNSpub

Effects of 60Co gamma radiation doses on seed germination of Jatropha curcas L.

Research Paper | November 1, 2018

| Download 18

Baudouin K. Nyembo, Alexandre N. Mbaya, Calvin C. Ilunga, Jean-Louis N. Muambi, Luc L. Tshilenge

Key Words:

Int. J. Agron. Agri. Res.13( 5), 46-52, November 2018


IJAAR 2018 [Generate Certificate]


This study aimed to assess the effects of different gamma radiation doses from Cobalt -60 isotopic source on seed germination and early growth parameters of Jatropha curcas L. Healthy and dry seeds were subjected to three doses of gamma rays (100, 200 and 300 Gy). The experiment was conducted using randomized complete block design, with three replicates. The significantly maximum germination percentage (89.85 %), seedling survival (92.3 %), seedling collar diameter (0.892 cm), plant height (17.30 cm), number of leaves (7) were observed at 30 days after germination. The results revealed that seed germination percentages and seedling shoot length decreased with increasing dose of gamma-rays. Higher gamma-ray dose (300 Gy) in particular had a pronounced effect on these germination parameters than others, probably because high-dose inhibited cell division due to free radicals and DNA system damage. The LD50 for seeds germination rates was obtained at 254 Gy. These results implied that germination traits of Jatropha curcas seeds were sensitive to increase in gamma-ray.


Copyright © 2018
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Effects of 60Co gamma radiation doses on seed germination of Jatropha curcas L.

Agbogidi OM, Akparobi SO, Eruotor PG. 2013. Health and environmental benefits of Jatropha curcas linn Applied Science Reports1 (2), 36-39.

Azhar M, Sobri H, Rusli I. 2009. Mutagenesis in Jatropha curcas for novel mutant lines variety. Proceedings of the 8th Malaysia Congress on Genetics, Genting Highlands, Malaysia.

Chaudhuri KS. 2002. A simple and reliable method to detect gamma irradiated lentil (Lens culinaris Medik.) seeds by germination efficiency and seedling growth test. Radiation Physics and Chemistry 64, 131-136.

Chiangmai P Na, Pootaeng-on Y, Meetum P, Jankomon N, Muangnoi D, Kitthip D. 2014. Mutation Induction in Physic Nut (Jatropha curcas L.) by Colchicine Treatments. Silpakorn U Sciences & Techiques Journal 8(2), 28-39.

Das S, Misra RC, Mahapatra AK, Gantayat  BP, Pattnaik RK. 2010. Genetic variability, character association, and path analysis in Jatropha curcas. World Applied Sciences Journal 8(11), 1304-1308.

De Villiers AJ, Van Rooym MW, Theron GK, Van Deventer HA. 1994. Germination of three namaqual and pioeer species, as influenced by salinity, yemperature and light.Seed Science and Technology 22, 427- 433.

Dias MP, Dias DCFS, Dias LAS. 2007. Germinação de sementes de pinhãomanso (Jatropha curcas L.) em diferentes temperaturas e substratos. In: II Congresso da RedeBrasileira de Tecnologia de Biodiesel, Brasília. Anais do II Congresso da Rede Brasileira de Tecnologia de Biodiesel. Brasília: MCT/ABIPIT, p, 1-5, 2007.

Divakara BN, Upadhyaya HD, Wani SP. Gowda CL, Laxmipathi. 2010. mutagenesis in Jatropha curcas L. to induce variability in seed germination, growth and yield traits. Romanian Journal of Biology – Plant Biology 55(2), 113-125, Bucharestest.

Dwimahyani I, Ishak. 2004. Induced mutation on Jatropha (Jatropha curcas L.) for improvement of agronomic characters variability, 53-60. www.digilib.batan.go.id/atomindonesia/fulltex/v30-n2-7-2004/Ita-Dwimahyani-Ishak.pdf.

Heller J. 1996. Physic nut Jatropha curcas L. promoting the conservation and use of underutilized and neglected crops. Institute of Plant Genetic and Crop Plant Research, Gatersleben/ International Plant Genetic Resource Institute, Rome, Italy.

Kambu K. 1990. Eléments de phytothérapie comparée. Plantes médicinales africaines. Centre de Recherches Pédagogiques Kinshasa. 105 p.

Khalil SJ, Rehman S, Afridi K, Jan MT. 1986. Damage induced by gamma irradiation in morphological and chemical characteristics of barley. Sarhad Journal of Agriculture 2, 45-54.

King A, He W, Cuevas J, Freudenberger M, Ramiaramanana D, Graham I. 2009.Potential of Jatropha curcas as a source of renewable oil and animal feed. Journal of Experimental Botany 60, 2897-2905.

Kovacs E, Keresztes A. 2002. Effect of gamma and UV-B/C radiation on plant cellule Micron 33: 199- 210.

Martinez-Herrera J, Siddhuraju P, Francis  G, D´ avila-Ort´ız G, Becker K. 2006. Chemical composition, toxic/antimetabolic constituents, and effects of different treatments on their levels, in four provenances of Jatropha curcas L. from Mexico. Food Chemistry 96, 80–89.

Martins CC, Machado CG, Cavasini R. 2008. Temperatura e substrato para o teste de germinação de sementes de pinhãomanso. Revista Ciência e Agrotecnologia, 32(3), 863-868, www.dx.doi.org/10.1590/S141370542008000300024

Nayak D, Patil NS, Jha SK, Jadeja DB. 2012. Gamma induced variability in Jatropha curcas L. Phytotechnology: Emerging Trends, Scientific Publishes, India, p 248-251.

Nayak D, Patil NS, Behera LK, Jadeja DB. 2015. Effects of gamma rays on germination and growth in Jatropha curcas L. Journal of Applied and Natural Science 7(2), 964 – 969.

Oliveira GL, Denise Cunha Fernandes dos Santos Dias, Paulo Cesar Hilst, Laércio Junio da Silva, Luiz Antônio dos Santos Dias. 2014 .Standard germination test in physic nut (Jatropha curcas L.) seeds. J.Seed Sci. 36(3), Londrina. http://dx.doi.org/10.1590/2317-1545v36n31015

Pandey RK. 2016. Effects of Gamma Rays on Cotyledonary leaves of Jatropha curcas L. Bulletin Environmental Pharmacology Life Science 5, 23-26.

Patade Y, Suprasanna P, Bapat VA. 2008. Gamma Irradiation of Embryo genic Callus Cultures and in vitro Selection for Salt Tolerance in Sugarcane (Saccharum officinaram L.). Agricultural Sciences in China 7(9), 1147 1152.

Prasad D, Reddy M, AmirahIzam, Md. Maksudur Rahman Khan. 2012. Jatropha curcas: Plant of medical benefits. Journal of Medicinal Plants Research 6(14), 2691-2699.

Sarhan SA. Amira Sh A, Soliman AZ, Rayan AO, El-Shishtawy H. 2015. Invitro genetic improvement of Jatropha curcas L using gamma ray to induce salinity tolerance. Life Science Journal; 12(5), 46-53]. (ISSN: 1097-8135).

Songsri P, Suriharn B, Sanitchon J, Srisawangwong S, Kesmala T. 2011. Effects of Gamma Radiation on Germination and Growth Characteristics of Physic Nut (Jatropha curcas L.). Journal of Biological Sciences 11, 268-274.

Shakoor A, Hassan M, Saleem M, Sadiq MS, Haq MA. 1978. Radio-sensitivity in four spring wheat varieties. The Nucleus. 15, 23-26.

Shark AY, Abo Remalia SIH, Abou El Enin MM. 2016. Genetic studies on Jatropha plant (Jatropha curcas L.) using different gamma radiation doses. 4(2), p. 207-213.

Surahman M, Santosa E, Agusta H, Aisyah SI, Nisya FN. 2018. Effects of gamma irradiation on the performance of Jatropha curcas L. accessions. IOP Conf. Ser.: Earth Environmental Science 141 012029.

Surwenshi Ashok, Vinod Kumar, Shanwad UK, Jalageri BR. 2011. Critical Review of Diversity in Jatropha curcas for Crop Improvement: A Candidate Biodiesel Crop Research Journal of Agricultural Sciences 2(2), 193-198.

Tatikonda L, Wani S, Kannan S, Beerelli N, Sreedevi T, Hoi- sington D, Devi P, Varshney R. 2009. AFLP-based molecular characterization of an elite germplasm collection of Jatropha curcas L.: a biofuel plant. Plant Science. 176, 505–513.

Zaka R, Chenal C, Misset MT. 2004.Effects of low doses of short term gamma irradiation on growth and development through two generations of Pisum sativam Science of the Total Environment 320, 121 129.