Effects of Annona squamosa leaf extract on Mus musculus exposed to mercury acetate

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Research Paper 01/07/2016
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Effects of Annona squamosa leaf extract on Mus musculus exposed to mercury acetate

Joycelyn C. Jumawan, Edna A. Amparado
Int. J. Biosci.9( 1), 255-267, July 2016.
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Abstract

The effects of solo and combinatorial exposure to ethanolic extract of Annona squamosa Linn. leaves and mercuric acetate (MA) were studied in gestating ICR mice and their fetuses in-utero.  Results show that mercuric acetate significantly increased maternal mortality and fetal resorption index while inducing decreased fetal size. Exposure of mercuric acetate and leaf extract doses significantly enhanced this effect. Solo exposure of ethanolic extract at 25 mg/kg body weight induced abortion and resorption of embryos to a lesser extent than combinatorial exposure. Lipid peroxidation assay reveal lower MDA values in liver and brain of maternal mice exposed to solo and combinatorial exposure but not with their fetal counterpart.  Maternal mice and fetuses exposed to leaf extract of A. squamosa with MA show marked hepatic and neurologic histological injury similar to positive control. The results suggest that A. squamosa leaves have the potential to protect against mercury-induced oxidative stress in maternal mice but may not be applied during gestation

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Ali SF, LeBel CP, Bondy SC. 1992. Reactive oxygen species formation as a biomarker of methylmercury and trimethyltin neurotoxicity. Neurotoxicology 13, 637-648.

Chang FR, Chen JL, Chiu HF, Wu MJ, Wu YC. 1998. Acetogenins from the seeds of Annona reticulata. Phytochemistry 47, 1057-1061. http://dx.doi.org/10.1016/S0031-9422(98)80072-1

Damasceno DC, Volpato GT, Sartori TCF, Rodriguez PF, Perin EA, Calderon IMP, Rudge MVC. 2002. Effects of Annona squamosa extract on early pregnancy in rats. Phytomedicine 9, 667- 672.          http://dx.doi.org/10.1078/094471102321616508

Ercal N, Gurer-Orhan H, Burns NY. 2001. Toxic metals and oxidative stress Part 1: Mechanisms involved in metal induced oxidative damage. Current Topics in Medicinal Chemistry 1, 529-539. http://dx.doi.org/org/10.2174/1568026013394831

Flora SJS, Mittal M, Mehta A. 2008. Heavy metal induced oxidative stress and its possible reversal by chelation therapy. Indian Journal of Medical Research. 128, 501–523.

Gale TF. 1974. Embryopathic effects of different routes of administration of mercuric acetate in the hamster. Environmental Research 8, 207–213. http://dx.doi.org/10.1016/0013-9351(74)90052-8

Glover GN, Zheng D, Jayashankar S, Sales GD, Hogstrand C, Lundbye AK. 2009. Methylmercury Speciation Influences Brain Gene Expression and Behavior in Gestationally-Exposed Mice Pups. Toxicological Science 110(2), 389-400. http://dx.doi.org/10.1093/toxsci/kfp105

Goswami M, Bijayalaxmi D, Dash NZ. 2011. Traditional Method of Reproductive Health Care Practices and Fertility Control among the Bhumija Tribe of Baleswar, Orissa. Ethnological Medicine. 5(1), 51-55.

Goyer RA. 1990. Transplacental transport of lead and mercury. Environmental Health Perspective89, 101–105.

Gupta RK, Kesari AN, Watal G, Murthy PS, Chandra R, Tandon V. 2005. Nutritional and hypoglycemic effect of fruit pulp of Annona squamosa in normal healthy and alloxan induced diabetic rabbits. Annals of Nutrition and Metabolism 88, 1244–1254. http://dx.doi.org/10.1159/000088987

Gupta RK, Kesari AN, Diwakar S, Tyagi A, Tandon V, Chandra R, Watal G. 2008. In vivo evaluation of antioxidant and antilipidimic potential of Annona squamosa aqueous extract in Type 2 diabetic models. Journal of Ethnopharmacology 118, 21-25. http://dx.doi.org/10.1016/j.jep.2008.03.008

Hopp D, Craig J, Allali M, Feras Q, Gu F, Zhi Ming L, McLaughlin G. 1998. Mono THF ring annonaceous acetogenins from Annona squamosa. Phytochemistry 47, 803-809. http://dx.doi.org/10.1016/S0031-9422(97)00822-4

Hussain T, Shukla GS, Chandra SF. 1987.The effects of cadmium and mercury on superoxide dismutase and lipid peroxidation in liver and kidney of growing rats: In vivo and in- vitro studies. Basic and Clinical Pharmacology and Toxicology 60, 355-358. http://dx.doi.org/10.1111/j.16000773.1987.tb01526.x

Kakita A, Wakabayashib K, Sua M, Yoneoka J, Sakamoto M, Ikuta F, Takahashi T. 2000. Intrauterine methylmercury intoxication Consequence of the inherent brain lesions and cognitive dysfunction in maturity. Brain Research. 877, 322–330. http://dx.doi.org/10.1016/S0006-8993(00)02717-7

Kempraj V, Bhat SK. 2011. Acute and reproductive toxicity of Annona squamosa to Aedes albopictus. Pesticide Biochemistry and Physiology 100(1), 82-86. http://dx.doi.org/10.1016/j.pestbp.2011.02.009

Khan AT, Atkinson A, Graham TC, Thompson SJ, Ali S, Shireen FS. 2004. Effects of inorganic mercury on reproductive performance of mice. Food and Chemical Toxicology. 42, 571-577. http://dx.doi.org/10.1016/j.fct.2003.10.018

Khera KS. 1974. Reproductive capability of male rats and mice treated with methyl mercury. Toxicology and Applied Pharmacology. 24, 167-177. http://dx.doi.org/10.1016/0041-008X(73)90136-1

Kumar RV, Reddy GV, Reddy MK. 2012. Medicinal Plants Having Fertility Related and Pharmacological Activities. International Journal of Pharma Medicine and Biological Sciences 1(1), 2-18.

Mishra A, Dogra JV, Singh JN, Jha OP. 1979. Post-coital antifertility activity of Annona squamosa and Ipomonea fistulosa. Planta Medica. 35, 283–285.

Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides by TBA reaction. Analytical Biochemistry. 95, 351–358. http://dx.doi.org/10.1016/0003-2697(79)90738-3

Patrick L. 2002. Mercury toxicity and antioxidants: part I: role of glutathione and alpha-lipoic acid in the treatment of mercury toxicity. Toxicology and Applied Pharmacology 7, 456-471

Punzalan-Brady GAN. 2008. Cancer chemopreventive and chemotherapeutic activities of flavoid-positive fractions from Annona squamosa Linn leaf extracts. PhD Dissertation (unpub).2008, College of Science, University of the Philippines Quezon City.

Punzalan GAN, Concepcion GP, Dator RP, Mazahery AR, Jacinto SD. 2006. Antioxidant activity of flavonoids and other polyphenols isolated from Annona squamosa Linn. leaf extracts. European Journal of Cancer Supplements 4, 58-62.

Quig D. 1998. Cystein metabolism and metal toxicity. Alternative Medicine Review 3(4), 262–270.

Raj DS. Vennila JJ, Aiyavu C, Panneerselvan K. 2009. The hepatoprotective effect of alcoholic extract of Annona squamosa leaves on experimentally induced liver injury in swiss albino mice. International Journal of Integrative Biology 5(3), 182-186.

Saleem TS, Christina AJM, Chidambaranathan N, Ravi V, Gauthaman K. 2008. Hepatoprotective activity of Annona squamosa Linn. on experimental animal model. International Journal of Applied Research in Natural Products. 1(3), 1-7.

Saleem TS, Sundarapandian R, Muthumanikkam A, Kalimuthu G, Parameswari A, Srinivas TRV, Karunakaran G. 2011. Protective effect of methanolic extract of Annona squamosa Linn in Izoniazid-Rifampicin induced Hepatotoxicity in Rats. Pakistan Journal of Pharmacological Sciences 24(2), 129–134.

Sausen de Freitas A, Funck VR, Santos Rotta M, Bohrer D, Mörschbächer V, Puntel RL, Nogueira CW, Farina M, Aschner M, Batista J, Rocha T. 2009. Diphenyl diselenide, a simple organoselenium compound, decreases methylmercury-induced cerebral, hepatic and renal oxidative stress and mercury deposition in adult mice. Brain Research Bulletin 79, 77–84. http://dx.doi.org/10.1016/j.brainresbull.2008.11.001

Shirwaikar A, Rajendran K, Kumar D, Bodla R. 2004. Antidiabetic activity of aqueous leaf extract of Annona squamosa in streptozotocin nicotinamide type 2 diabetic rats. Journal of Ethnopharmacology. 91, 171–175. http://dx.doi.org/10.1016/j.jep.2003.12.017

Stringari J, Nunes AKC, Franco JL, Bohrer D, Garcia SC, Dafre AL, Milatovic D, Souza DO, Rocha JBT, Aschner M, Farina M. 2008. Prenatal methymercury exposure hampers glutathione antioxidant system ontogenesis and causes long-lasting oxidative stress on mouse brain. Toxicology and Applied Pharmacology  227(1), 147-154. http://dx.doi.org/10.1016/j.taap.2007.10.010

Strubelt O. 1996. Comparative studies on the toxicity of mercury, cadmium, and copper toward the isolated perfused rat liver. Journal of Toxicology and Environmental Health, Part A 47(3), 267-283. http://dx.doi.org/10.1080/009841096161780

Woo S, Yum S, Park HS, Lee TK, Ryu JC.2009. Effects of heavy metals on antioxidants and stress-responsive gene expression in Javanese medaka Oryzias javanicus. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology. 149(3), 289-299. http://dx.doi.org/10.1016/j.cbpc.2008.08.002

Yoshida M, Satoh M, Shimada A, Yamamoto  E, Yasutake A, Tohyama C. 2002.Maternal-to-fetus transfer of mercury in metallothionein-null pregnant mice after exposure to mercury vapour. Toxicology 175, 215–222. http://dx.doi.org/10.1016/S0300-483X(02)00084-7

Yoshida  M. 2002.Placental to Fetal Transfer of Mercury and Fetotoxicity. Tohoku Journal of Experimental Medicine 196, 79–88. http://dx.doi.org/10.1620/tjem.196.79