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Effects of Aspergillus flavus inoculation on spring Maize (Zea mays L.) grain yield

Sadia Zafar, Muhammad Ali, Naeem Iqbal, Khalid Aziz, Muhammad Zulqurnain Haider, Muhammad Rafique

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Int. J. Biosci.13( 4), 24-35, October 2018

DOI: http://dx.doi.org/10.12692/ijb/13.4.24-35


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Maize (Zea mays L.) is a staple crop and widely consumed over the globe, but its production is very challenging due to its contamination by Aspergillus species. Aspergillus flavus is the most dominant fungi that produce aflatoxin in many major crops. Aflatoxin producing fungi mostly attack during the development of crops and induce infection. Present study was aimed to investigate the effects of A. flavus inoculation on grain yield of maize genotypes in open field environments at pre-harvest time. Fourteen maize genotypes were treated with A. flavus inoculum by non-wounding method (inoculated through cob silk), at grain filling stage and yield related parameters were recorded. Results indicated that maize genotype R-3305 produced higher values for cob length and number of grains per cob. Maize genotype FH-949 produced higher values for cob diameter and cob weight. Maize genotype FH-1046 produced higher value for total grains weight per cob and KSC-9663 produced higher values for 100 grains weight as compared to all other genotypes.HC-2040 produced lower values for yield related parameters as compared to all other genotypes. Based on the results of current work, it can be concluded that A. flavus inoculation affected the grain yield related parameters. The maize genotype, FH-949 and FH-1046produced better grain yield as compared to all other genotypes. Thus A. flavus inoculation affected the grain yield related component and had a pronounced role in the reduction of maize production.


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Effects of Aspergillus flavus inoculation on spring Maize (Zea mays L.) grain yield

Abbas H, Shier W, Cartwright R. 2007. Effect of temperature, rainfall and planting date on aflatoxin and fumonisin contamination in commercial Bt and non-Bt corn hybrids in Arkansas. Phytoprotection 88, 41-50. http://dx.doi.org/10.7202/018054ar

Afarinesh A, Farshadfar E, Choukan R. 2005. Genetic analysis of drought tolerance in maize (Zea mays L.) using diallel method. Seed Plant 20, 457- 473.

Ali Q, Ahsan M, Ali F, Aslam M, Khan NH, Munzoor M, Mustafa HSB, Muhammad S, 2013. Heritability, heterosis and heterobeltiosis studies for morphological traits of maize (Zea maysL.) seedlings. Advancements in Life Sciences 1, 52-63.

Ali Q, Ahsan M, Khan NH, Ali F, Elahi M, Elahi F. 2012b. Genetic analysis for various quantitative traits of chickpea (Cicer arietinum L.). International Journal for Agro Veterinary and Medical Sciences 6, 51-57. http://dx.doi.org/10.5455/ijavms.12877

Ali Q, Ahsan M, Tahir MHN, Basra SMA. 2012a. Genetic evaluation of maize (Zea mays L.) accessions for growth related seedling traits. International Journal for Agro Veterinary and Medical Sciences 6, 164-172. http://dx.doi.org/10.5455/ijavms.133

Ali Q, Ahsan M. 2015. Correlation analysis for various grain contributing traits of Zea mays. African Journal of Agricultural Research 10, 2350-2354. http://dx.doi.org/10.5897/AJAR2013.7838

Ali Q, Elahi M, Ahsan M, Tahir MHN, Basra SMA. 2011. Genetic evaluation of maize (Zea mays L.) genotypes at seedling stage under moisture stress. International Journal for Agro Veterinary and Medical Sciences 5, 184-193.

Amaike S, Keller NP. 2011. Aspergillus flavus. Annual Review of Phytopathology 49, 107-133. https://doi.org/10.1146/annurev-phyto-072910-095221

Ashraf U, Salim MN, Sher A, Sabir SUR, Khan A, Pan SG, Tang X. 2016. Maize growth, yield formation and water-nitrogen usage in response to varied irrigation and nitrogen supply under semi-arid climate. Turkish Journal of Field Crops 21(1), 88-96. http://dx.doi.org/10.17557/tjfc.93898

Atanda O, Ogunrinu M, Olorunfemi F. 2011. A neutral red desiccated coconut agar for rapid detection of aflatoxigenic fungi and visual determination of aflatoxins. World Mycotoxin Journal 4(2), 147-155. https://doi.org/10.3920/WMJ2010.1241

Bellaloui N, Abbas HK, Bruns HA, Mengistu A. 2016.Grain Chemical Composition as Affected by Genetic Backgrounds and Toxigenic Aspergillus flavus Inoculation in Corn Hybrids. Atlas Journal of Plant Biology 66-76. https://doi.org/10.5147/ajpb.v0i0.115

Bruns HA, Abbas HK. 2005. Responses of short-season corn hybrids to a humid subtropical environment. Agronomy Journal 97, 446-451. http://dx.doi.org/10.2134/agronj2005.0446

Bruns HA, Abbas HK. 2006. Planting date effects on Bt and non-Bt corn in the mid-south USA. Agronomy Journal 98, 100-106. http://dx.doi.org/10.2134/agronj2005.0143

Campa De La R, Hooker DC, Miller JD, Schaafsma AW, Hammond BG. 2005. Modeling effects of environment, insect damage, and Bt genotypes on fumonisin accumulation in maize in Argentina and the Philippines. Mycopathologia 159, 539-552.

Christensen SA, Kolomiets MV. 2011. The lipid language of plant–fungal interactions. Fungal Genetics and Biology 48(1), 4-14. https://doi.org/10.1016/j.fgb.2010.05.005

Farfan IDB, Gerald N, Murray SC, Isakeit T, Huang PC, Warburton, Williams P, Windham GL,  Kolomiets M. 2015. Genome wide association study for drought, aflatoxin resistance, and important agronomic traits of maize hybrids in the sub-tropics.  PLoS One 10(2), e0117737. https://doi.org/10.1371/journal.pone.0117737

Fountain J, Scully B, Ni X, Kemerait R, Lee D, Chen ZY, Guo B. 2014. Environmental influences on maize-Aspergillus flavus interactions and aflatoxin production. Frontiers in Microbiology 5, 40. https://doi.org/10.3389/fmicb.2014.00040

Foyer CH, Noctor G. 2003. Redox sensing and signalling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. Physiologia Plantarum 119(3), 355-364. https://doi.org/10.1034/j.1399-3054.2003.00223.x

Gong M, Chen BO, Li ZG, Guo L H. 2001. Heat-shock-induced cross adaptation to heat, chilling, drought and salt stress in maize seedlings and involvement of H2O2. Journal of Plant Physiology 158(9), 1125-1130. https://doi.org/10.1078/0176-1617-00327

Grzesiak MT, Rzepka A, Hura T, Hura K, Skoczowski A. 2007. Changes in response to drought stress of triticale and maize genotypes differing in drought tolerance. Photosynthesis 45, 280-287.

Hasyan RM, Moualla YM, Ahmad AAS. 2012. Potence ratio and path coefficient analysis for some quantitative traits of maize (Zea maysL.) hybrids developed in Syria. Jordan Journal of Agricultural Sciences 8, 557-565.

Hiran P, Kerdchoechuen O, Laohakunjit N. 2016. Combined effects of fermentation and germination on nutritional compositions, functional properties and volatiles of maize seeds. Journal of Cereal Science 71, 207-216. https://doi.org/10.1016/j.jcs.2016.09.001

Ilker E. 2011.Correlation and path coefficient analyses in sweet corn. Turkish Journal of Field Crops 16, 105-107.

Karasu A, Kuscu H, Mehmet OZ, Bayram G. 2015. The Effect of Different Irrigation Water Levels on Grain Yield, Yield Components and Some Quality Parameters of Silage Maize (Zea mays in dentate Sturt.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca 43, 138-145. http://dx.doi.org/10.15835/nbha4319602

Kelley RY, Williams WP, Mylroie JE, Boykin DL, Harper JW, Windham GL,  Ankala A, Shan X. 2012. Identification of maize genes associated with host plant resistance or susceptibility to Aspergillus flavus infection and aflatoxin accumulation. PLoS One 7(5), e36892. https://doi.org/10.1371/journal.pone.0036892

Khan NA, Yu P, Ali M, Cone JW, Hendriks WH. 2015. Nutritive value of maize silage in relation to dairy cow performance and milk quality. Journal of the Science of Food and Agriculture 95(2), 238-252. https://doi.org/10.1002/jsfa.6703

Klich MA. 2002. Identification of common Aspergillus species. Centraalbureau Voor Schimmel cultures.

Kuhnen S, Ogliari JB, Dias PF, Boffo EF, Correia I, Ferreira AG, Delgadillo I, Maraschin M. 2010. ATR-FTIR spectroseopy and chemo-metric analysis applied to discrimination of landrace maize flours produced in southern Brazil. International Journal of Food Science & Technology 45(8), 1673-1681. https://doi.org/10.1111/j.1365-2621.2010.02313.x

Kumar GP, Prashanth Y, Reddy VN, Sudheer S, Rao VP. 2014. Character association and path coefficient analysis in maize (Zea mays L.). International Journal of Applied & Biology and Pharmaceutical Technology 5, 257-260.

Liu Y, Wu F. 2010. Global burden of aflatoxin-induced hepatocellular carcinoma: a risk assessment. Environmental Health Perspectives 118 (6), 818. http://dx.doi.org/10.1289/ehp.0901388

Maina AW, Wagacha JM, Mwaura FB, Muthomi JW, Woloshuk CP. 2016. Postharvest practices of maize farmers in Kaiti District, Kenya and the impact of hermetic storage on populations of Aspergillus spp. and aflatoxin contamination. Journal of Food Research 5(6), 53.

Mayfield K, Betrán FJ, Isakeit T, Odvody G, Murray SC, Rooney WL, Landivar JC. 2012. Registration of maize germplasm lines Tx736, Tx739, and Tx740 for reducing pre-harvest aflatoxin accumulation. Journal of Plant Registrations 6(1), 88-94. http://dx.doi.org/10.3198/jpr2010.12.0675crg

Ogbaga CC, Miller MA, Johnson GN. 2017. Fourier transform infrared spectroscopic analysis of maize (Zea mays) subjected to progressive drought reveals involvement of lipids, amides and carbohydrates. African Journal of Biotechnology 16 (18), 1061-1066. https://doi.org/10.5897/AJB2017.15918

Pastori GM, Kiddle G, Antoniw J, Bernard S, Veljovic-Jovanovic S, Verrier PJ, Noctor G, Foyer CH. 2003. Leaf vitamin C contents modulate plant defense transcripts and regulate genes that control development through hormone signaling. The Plant Cell 15(4), 939-951. https://doi.org/10.1105/tpc.010538

Patt AG, Schröter D. 2008. Perceptions of climate risk in Mozambique: implications for the success of adaptation strategies. Global Environmental Change 18(3), 458-467. https://doi.org/10.1016/j.gloenvcha.2008.04.002

Probst C, Schulthess F, Cotty PJ. 2010. Impact of Aspergillus section Flavi community structure on the development of lethal levels of aflatoxins in Kenyan maize (Zea mays). Journal of Applied Microbiology 108(2), 600-610. https://doi.org/10.1111/j.1365-2672.2009.04458.x

Rani GU, Rao VS, Ahmad ML, Rao KN. 2017. Character association and path coefficient analysis of grain yield and yield components in maize (Zea mays L.). International Journal of Current Microbiology and Applied Sciences 6, 4044-4050. https://doi.org/10.20546/ijcmas.2017.612.465

Schilling J, Vivekananda J, Khan MA, Pandey N. 2013.Vulnerability to environmental risks and effects on community resilience in mid-west Nepal and south-east Pakistan. Environment and Natural Resources Research 3(4), 27.

Shakoor MB, Nawaz R, Hussain F, Raza M, Ali S, Rizwan M, Oh SE, Ahmad S. 2017. Human health implications, risk assessment and remediation of As-contaminated water: A critical review. Science of the Total Environment 601, 756-769. https://doi.org/10.1016/j.scitotenv.2017.05.223

Smit B, Skinner MW. 2002. Adaptation options in agriculture to climate change: a typology. Mitigation and Adaptation Strategies for Global Change 7(1), 85-114.

Wagacha JM, Muthomi JW. 2008. Mycotoxin problem in Africa: current status, implications to food safety and health and possible management strategies. International Journal of Food Microbiology 124(1), 1-12. https://doi.org/10.1016/j.ijfoodmicro.2008.01.008

Wahid A, Perveen M, Gelani S, Basra SM. 2007. Pretreatment of seed with H2O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. Journal of Plant Physiology 164(3), 283-294. https://doi.org/10.1016/j.jplph.2006.01.005

Wang BQ, Li ZH, Duan LS, Zhai ZX. 2007. Effect of coronatine on photosynthesis parameters and endogenous hormone contents in maize (Zea mays L.) seedling under drought stress. Plant Physiology Communications 43, 269-272.

Williams WP, Windham GL. 2012. Registration of Mp718 and Mp719 germplasm lines of maize. Journal of Plant Registrations 6, 200-202. http://dx.doi.org/10.3198/jpr2011.09.0489crg

Windham GL, Williams WP. 2016. Effect of inoculum concentrations of Aspergillus flavus and A. parasiticus on aflatoxin accumulation and kernel infection in resistant and susceptible maize hybrids. Phytoparasitica 44, 333-339. http://dx.doi.org/10.1007/s12600-016-0523-4

Windham GL, Williams WP. 2007. A comparison of inoculation techniques for inducing aflatoxin contamination and Aspergillus flavus kernel infection on corn hybrids in the field. Phytoparasitica 35, 244.

Windham GL, Williams WP. 2016. Effect of inoculum concentrations of Aspergillus flavus and A. parasiticus on aflatoxin accumulation and kernel infection in resistant and susceptible maize hybrids. Phytoparasitica 44, 333-339.

Wu F, Munkvold GP. 2008. Mycotoxins in ethanol co-products: modeling economic impacts on the livestock industry and management strategies. Journal of Agricultural and Food Chemistry 56(11), 3900-3911. http://dx.doi.org/10.1021/jf072697e