Effects of cadmium on some morphological and physiological traits of amaranth (Amaranthus caudatus L.) and oilseed rape (Brassica napus L.)

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Effects of cadmium on some morphological and physiological traits of amaranth (Amaranthus caudatus L.) and oilseed rape (Brassica napus L.)

E. Benyas, A. Dabbagh Mohammadi Nassab, S. Oustan
Int. J. Biosci.3( 4), 17-26, April 2013.
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In order to investigate cadmium (Cd) effects on some morphological and physiological traits of amaranth (Amaranthus caudatus L.) and oilseed rape (Brassica napus L.) on experiment as Randomized Complete Block Design with six replications was carried out in greenhouse. Treatments were 10, 50 and 100 mg/kg Cd with control. Results showed that Cd Treatments decreased morphological traits such as number, area and dry weight of green leaves in both oilseed rape and amaranth. Also, shoot and root dry weight and plant height of oilseed rape reduced. Different Cd concentrations had a significant effect on F0, chlorophyll fluorescence, chlorophyll a, b and total and carotenoid contents in amaranth. At concentration of 100 mg/kg Cd destructive effects on pigment content and chlorophyll fluorescence was observed in amaranth. Cd had no significant effect on chlorophyll fluorescence and pigment content in oilseed rape. Chlorophyll a and total had significantly negative correlation with SLA for amaranth. Anthocyanin content and chlorophyll a/b ratio were not affected by Cd stress in both amaranth and oilseed rape.


Anderson IC, Robertson DS. 1960. Role of carotenoids in protecting chlorophyll from photodestruction. Plant Physiology 35, 531–534. http://dx.doi.org/10.1104/p.35.4.531

Baryla A, Carrier P, Franck F, Coulomb C, Sahut C, Havaux M. 2001. Leaf chlorosis in oilseed rape plants (Brassica napus L.) grown on cadmium-polluted soil: causes and consequences for photosynthesis and growth. Planta 212, 696–709.

Bosiacki M, Wojciechowska E. 2012. Phytoextraction of nickel by selected ornamental plants. Ecological Chemistry and Engineering S 19, 331–345. http://dx.doi.org/10.2478/v10216-011-0024-9

Chen X, Wang J, Shi Y, Zhao MQ, Chi, GY. 2011. Effects of cadmium on growth and photosynthetic activities in pakchoi and mustard. Botanical Studies 52, 41–46.

Chen Y, Huerta AJ. 1997. Effects of sulfur nutrition on photosynthesis in cadmium-treated barley seedlings. Journal of Plant Nutrition 20, 845–856. http://dx.doi.org/10.1080/01904169709365300

Drazkiewicz  A,  Tukendorf  A,  Baszyñski  T. 2003. Age-dependent response of maize leaf segments to cadmium treatment: Effect on chlorophyll fluorescence and phytochelatin accumulation. Journal of Plant Physiology 160, 247–254.

Dushenkov  S,  Mikheev  A,  Prokhnevsky  A, Ruchko M, Sorochinsky B. 1999. Phytoremediation of radiocesium-contaminated soil in the vicinity of Chernobyl, Ukraine. Environmental Science and Technology 33, 469–475. http://dx.doi.org/10.1021/es980788

Eshghi S, Mahmoodabadi MR, Abdi GR, Jamali B. 2010. Zeolite Ameliorates the Adverse Effect of Cadmium Contamination on Growth and Nodulation of Soybean Plant (Glycine max L.). Journal of Biological & Environmental Sciences 4, 43–50.

Ghani A. 2010. Effect of Cadmium Toxicity on the Growth and Yield Components of Mungbean [Vigna radiata (L.) Wilczek]. World Applied Sciences Journal 8, 26–29.

Greger M, Ogren E. 1991. Direct and indirect effects of Cd2+ on photosynthesis in sugar beet (Beta vulgaris). Physiologia Plantarum 83, 129–135. http://dx.doi.org/10.1111/j.1399-3054.1991.tb01291.x

Gussarson M, Asp, H, Adalsteinsson S, Jensén P. 1996. Enhancement of cadmium effects on growth and nutrient composition of birch (Betula pendula) by buthionine sulphoximine (BSO). Journal of Experimental Botany 47, 211–215. http://dx.doi.org/10.1093/jxb/47.2.211

Hegedus A, Erdi S, Horvath G. 2001. Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedling under cadmium stress. Plant Science 160, 1085–1093. http://dx.doi.org/10.1016/S0168-9452(01)00330-2

Jia L, Liu Z, Chen W, He X. 2012. Stimulative effect induced by low-concentration Cadmium in Lonicera japonica Thunb. African Journal of Microbiology Research 6, 826–833. http://dx.doi.org/10.5897/AJMR11.1337

Kuboi T, Noguchi A, Yazaki, J. 1986. Family-dependent cadmium accumulation characteristics in higher plants. Plant and Soil 92, 405–415. http://dx.doi.org/10.1007/BF02372488

Larsson E, Bornman J, Asp H. 1998. Influence of UV-B radiation and Cd2+ on chlorophyll fluorescence, growth and nutrient content in Brassica  napus.  Environmental  and  Experimental Botany 49, 1031–1039. http://dx.doi.org/10.1093/jxb/49.323.1031

Lefèvre I, Marchal G, Meerts P, Corréal E, Lutts S. 2009. Chloride salinity reduces cadmium accumulation by the Mediterranean halophyte species Atriplex halimus L. Environmental and Experimental Botany 65,142–152. http://dx.doi.org/10.1016/j.envexpbot.2008.07.005

Melo LCA, Alleoni LRF, Carvalho G, Azevedo RA. 2011. Cadmium- and barium-toxicity effects on growth and antioxidant capacity of soybean (Glycine max L.) plants, grown in two soil types with different physicochemical properties. Journal of Plant Nutrition and Soil Science 174, 847–859. http://dx.doi.org/10.1002/jpln.201000250

Milone MT, Sgherri C, Clijsters H, Navari-Izzo  F.  2003.  Antioxidative  responses  of  wheat treated with realistic concentration of cadmium. Environmental and Experimental Botany 50, 265–276. http://dx.doi.org/10.1016/S0098-8472(03)00037-6

Moteshare Zadeh B, Firozabadi GR, Alikhani HA, Mirseyed Hosseini H. 2008. Effect of sunflower and Amaranthus culture and application of inoculants on phytoremediation of the soil contaminated with cadmium. American-Eurasian Journal of Agricultural and Environmental Sciences 4, 93–103.

Moustakas M, Lanaras T, Symeonidis L, Karataglis S. 1994. Growth and some photosynthetic characteristics of field grown Avena sativa under copper and lead stress. Photosynthetica 30, 389–396.

Nouairi I, Ben Ammar W, Ben Youssef N, Ben Miled Daoud D, Ghorbal MH, Zarrouk M. 2006. Comparative study of cadmium effects on membrane lipid composition of Brassica juncea and Brassica napus leaves. Plant Science 170, 511–519. http://dx.doi.org/10.1016/j.plantsci.2005.10.003

Ouzounidou G, Moustakes M, Eleftheriou EP. 1997. Physiological and ultrastructural effects of cadmium on wheat (Triticum aestivum L.) leaves. Archives of Environmental Contamination and Toxicology 32, 154–160.

Pagliano C, Raviolo M, Vecchia FD, Gabbrielli R, Gonneli C, Rascio N, Barbato R, Rocca N.2006.  Evidence  for  PSII  donor-side  damage  and photoinhibition induced by cadmium treatment on rice (Oryza sativa L.). Photochemistry and Photobiology 84, 70–78. http://dx.doi.org/10.1016/j.jphotobiol.2006.01.012

Pál M, Horváth E, Janda T, Páldi E, Szalai G. 2006. Physiological changes and defense mechanisms induced by cadmium stress in maize. Journal  of  Plant  Nutrition  and  Soil  Science  169, 239–246. http://dx.doi.org/10.1002/jpln.200520573

Prasad MNV, Freitas H. 2003. Metal hyperaccumulation in plants – Biodiversity prospecting for phytoremediation technology. Electronic Journal of Biotechnology 6, 275–321 http://dx.doi.org/10.2225/vol6-issue3-fulltext-6

Ren A, Gao Y, Zhang L, Xie F. 2006. Effects of cadmium on growth parameters of endophyte-infected and endophyte-free ryegrass. Journal of Plant Nutrition and Soil Science 169, 857–860. http://dx.doi.org/10.1002/jpln.200520543

Salt DE, Prince RC, Pickering IJ, Raskin I. 1995. Mechanisms of cadmium mobility and accumulation in Indian mustard. Plant Physiology 109, 1427–1433. http://dx.doi.org/10.1104/pp.109.4.1427

Schreiber U. 1983. Chlorophyll fluorescence yield changes as a tool in plant physiology. I. The measuring system. Photosynthesis Research 4, 361–371.http://dx.doi.org/10.1007/BF00054144

Sheoran I, Singal H, Singh R. 1990. Effect of cadmium and nickel on photosynthesis and the enzymes of the photosynthetic carbon reduction cycle in pigeonpea (Cajanus cajan L.). Photosynthesis Research 23, 345–351. http://dx.doi.org/10.1007/BF00034865

Shi G, Liu C, Cai Q, Liu Q, Hou C. 2010. Cadmium accumulation and tolerance of two safflower cultivars in relation to photosynthesis and antioxidantive enzymes. Bulletin of Environmental Contamination and Toxicology 85, 256–263. http://dx.doi.org/10.1007/s00128-010-0067-0

Siedlecka A, Baszynski T. 1993. Inhibition of electron flow around photosystem I in chloroplasts of Cd-treated maize plants is due to Cd-induced iron deficiency. Physiologia Plantarum 87, 199–202. http://dx.doi.org/10.1111/j.1399-3054.1993.tb00142.x

Sims DA, Gamon JA. 2002. Relationships between leaf pigment content and spectral reflectance across a wide range of species, leaf structures and developmental stages. Remote Sensing of Environment 81, 337–354. http://dx.doi.org/10.1016/S0034-4257(02)00010-X

Skorzynska-Polit E, Tukendorf A, Selstam E, Baszynski T. 1998. Calcium modifies Cd effect on runner bean plants. Environmental and Experimental Botany 40, 275–286. http://dx.doi.org/10.1016/S0098-8472(98)00045-8

Stobart AK, Griffiths WT, Ameen-Bukhari I, Sherwood RP. 1985. The effect of Cd2+ on the biosynthesis of chlorophyll in leaves of barley. Physiologia Plantarum 63, 293–298. http://dx.doi.org/10.1111/j.1399-3054.1985.tb04268.x

Stritsis C, Steingrobe B. 2012. Shoot cadmium concentration of soil-grown plants as related to their root properties. Journal of Plant Nutrition and Soil Science 175, 456–465. http://dx.doi.org/10.1002/jpln.201100336

Turan M, Esringu A. 2007. Phytoremediation based on canola (Brassica napus L.) and Indian mustard (Brassica juncea L.) planted on spiked soil by aliquot amount of Cd, Cu, Pb and Zn. Plant, Soil and Environment 53, 7–15.

Van Assche F, Clijsters C. 1990. Effects of metals on enzyme activity in plants. Plant Cell and Environment 13, 195–206. http://dx.doi.org/10.1111/j.1365-3040.1990.tb01304.x

Vatehová Z, Kollárová K, Zelko I, Richterová- Kučerová D, Bujdoš M, Lišková D. 2012. Interaction of silicon and cadmium in Brassica juncea and Brassica napus. Biologia 67, 498–504. http://dx.doi.org/10.2478/s11756-012-0034-9

Wang JQ, Su DC. 2005. Distribution of cadmium in oilseed rape and Indian mustard grown on cadmium contaminated soil. Journal of Environmental Sciences (China) 17, 572–575.