Int. J. Biosci.2(12), 57-64, December 2012
This experiment was conducted to evaluate the combined effects of ethylene di amine tetra acetic acid (EDTA) and microbial phytase (MP) on digestibility of calcium and phosphorus and mineralization parameters of tibia bone in broilers chicks. This experiment was conducted using 360 Ross-308 male broiler chicks. In a completely randomized design with a 3×2 factorial arrangement (0, 0.1 and 0.2% EDTA and 0 and 500 FTU MP). Four replicate of 15 chicks per each were fed dietary treatments including (i) P-deficient basal diet [0.2% available phosphorus (aP)] (NC); (ii) NC + 500 FTU MP per kilogram of diet; (iii) NC + 0.1% EDTA per kilogram of diet; (iv) NC + 0.1% EDTA + 500 FTU MP per kilogram of diet; (v) NC + 0.2% EDTA per kilogram; and (vi) NC + 0.2% EDTA + 500 FTU MP per kilogram of diet. The content of calcium, phosphorus and length of tibia bone, digestibility of calcium and phosphorus was evaluated. The results showed that interaction effect of EDTA×MP on tibia calcium content to low available phosphorus diets was significant (p<0.01). Adding of EDTA to P-deficient diets, was increased tibia phosphorus content of broilers in compared with control group (p<0.001). Adding MP to P-deficient diets, based on corn-soybean meal, cause increased digestibility of phosphorus and also, length of tibia in broilers (p<0.0001). From this study it could be deduced that adding of MP to low available phosphorus diets can cause improvement of utilization of phytate phosphorus. Also adding EDTA as a chelator to diet can improve tibia mineralization parameters in broilers.
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