Effects of combined vitamins C and E on the growth performance and some antioxidant production levels in Clarias gariepinus (Burchell, 1822) fingerlings

Paper Details

Research Paper 01/02/2022
Views (444) Download (49)
current_issue_feature_image
publication_file

Effects of combined vitamins C and E on the growth performance and some antioxidant production levels in Clarias gariepinus (Burchell, 1822) fingerlings

Patrick Ozovehe Samuel, Merit Ile-anaju Uwada
Int. J. Biosci.20( 2), 329-341, February 2022.
Certificate: IJB 2022 [Generate Certificate]

Abstract

The demand for improved aquacultural practices is on constant increase to meet the demands of the teeming population of the human race at the lowest cost possible. The effects of combined vitamins C and E on the growth performance, catalase (CAT) and reduced glutathione (GSH) production levels in Clarias gariepinus fingerlings (10.00±0.00g) were evaluated for 12 week. 120 samples of the fish were assigned to 4 treatments thus: 00 (control), 200mg/L (T1), 300mg/L (T2) and 400mg/L (T3). The total length (TL), standard length (SL) and weight of the fish were determined weekly. The percentage weight gain (%W), specific growth rate (SGR) were also calculated. The kidneys, liver and gills were excised, homogenized in phosphate buffer; and then assayed for CAT and GSH every 4th week of the experiment. From the results, the TL and SL increased significantly with increase in the concentration of the combined vitamins especially in T2 and T3 much better than the control. The weight increased significantly from T1-T3 all through the 12 weeks with 120.88±28.75g as the highest, 1109%WG and SGR of 5.686g/day obtained in T3. The CAT and GSH in the kidneys, liver and gills decreased with increase in the concentrations of the combined vitamins and duration of the experiment with the control values significantly higher than all the treatments in most cases. The results from this research have shown significant improvements in growth and physiology of C. gariepinus and therefore, administration of these combined vitamins can serve as invaluable addition in catfish farming.

VIEWS 51

AbdelRahman AN, El-Hady M, Shalaby SI. 2019. Efficacy of the dehydrated lemon peels on the immunity, enzymatic antioxidant capacity and growth of Nile tilapia (Oreochromis niloticus) and African catfish (Clarias gariepinus). Aquaculture 505, 92–97.

AbdelRahman AN, Khalil AA, Abdallah HM, ElHady M. 2018. The effects of the dietary supplementation of Echinacea purpurea extract and/or vitamin C on the intestinal histomorphology, phagocytic activity, and gene expression of the Nile tilapia. Fish Shellfish Immunology 82, 312–318.

Adewolu MA, Adeniji CA, Adejobi AB. 2008. Feed utilization, growth and survival of Clarias gariepinus (Burchell, 1822) fingerlings cultured under different photoperiods. Aquaculture 283, 64-67.

Aebi H. 1984. Catalase in Vitro, Methods in Enzymology. Elsevier, pp. 121–126.

Ahmad I, Maria VL, Oliveira M, Serafim A, Bebianno MJ, Pacheco M, Santos MA. 2008. DNA damage and lipid peroxidation vs. protection responses in the gill of Dicentrarchus labrax L. from a contaminated coastal lagoon (Ria de Aveiro, Portugal). Science of the Total Environment 406, 298-307.

Ahmad Z. 2012. Toxicity bioassay and effects of sub-lethal exposure of malathion on biochemical composition and haematological parameters of Clarias gariepinus. African Journal of Biotechnology 11(34), 8578 -8585.

Ai Q, Mai K, Zhang C, Xu W, Duan Q, Tan B, Liufu Z. 2004. Effects of dietary vitamin C on growth and immune response of Japanese seabass, Lateolabrax japonicus. Aquaculture 242, 489- 500.

Alkaladi A. 2019. Vitamins E and C ameliorate the oxidative stresses induced by zinc oxide nanoparticles on liver and gills of Oreochromis niloticus. Saudi Journal of Biological Sciences 26(2), 357–362. https://doi.org/10.1016/j.sjbs.2018.07.001.

Aluta UP, Ademola ZA, Muyideen OL. 2021. Inclusion effects of onion peel powder in the diet of African catfish, Clarias gariepinus: Growth, blood chemistry, hepatitis antioxidant enzymes activities and SOD MRNA responses. Scientific African 12, 7-8.

Ayoola SO, Idowu AA, Babalola OE, Ademoye MK. 2014. Heavy metals accumulation and Enzymatic Biomarker in Commercially Important Fish Species (Hemichromis fasciatus and Chrysichthys nigrodigitatus) at the Landing Site, Bariga, Lagos Lagoon. World Applied Sciences Journal 29(2), 298-306. DOI:10.5829/idosi. wasj.2014.29.02.1628.

Azeez OI, Braimah SF. 2020. Mitigating Effect of Vitamin-E on Copper Sulphate-Induced Toxicity in African Catfish (Clarias gariepinus). European Journal of Medical and Health Sciences 2(4). https://doi.org/10.24018/ejmed.2020.2.4.411.

Bebianno MJ, Geret F, Hoarau P, Serafim MA, Coelho MR, Gnassia-Barelli M, Romeo M. 2014. Biomarkers in Ruditapes decussates: a potential bioindicator species. Biomarkers 9, 305-310.

Damsgaard CT, Lauritzen L, Kjaer TMR, Holm PMI, Fruelkilde MB, Michaelsen KF, Frokiaer H. 2007. Fish oil supplementation modulates immune function in healthy infants. The Journal of Nutrition 137(4), 1031-1036. https://doi.org/10.1093 /jn/137.4.1031.

Eboh AS. 2014. Biochemistry of free radicals and antioxidants. Scholars Academic Journal of Bioscience 2(2), 11 0-118.

Ellman GL. 1959. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics 82, 70-77.

Fadime EP. 2017. Vitamin C: An Antioxidant Agent. IntechOpen. DOI: 10.5772/intechopen.69660.

FAO. 2003. Food Security: concepts and measurement. Rome: Food and Agriculture Organization of the United Nations. In FAO (Ed.), Trade Reforms and Food Security, 25-34.

FAO. 2018. The state of world fisheries and aquaculture. FAO: Rome, Italy. P#227.

Faramarzi M. 2012. Effect of dietary vitamin C on growth and feeding parameters, carcass composition and survival rate of common carp (Cyprinus carpio). Global Veterinary 8, 507-510.

Fracalosi MD, Allen ME, Yuyama LK, Oftedal OT. 2001. Ascorbic acid biosynthesis in Amazonian fishes. Aquaculture 192, 321-332.

Gasco L, Gai F, Maricchiolo G, Genovese L, Ragonese S, Bottari T, Caruso G. 2018. Supplementation of vitamins, minerals, enzymes and antioxidants in fish feeds. In: Gasco L, Gai F, Maricchiolo G, Genovese L, Ragonese S, Bottari T, Caruso G., Eds. Feeds for the aquaculture sector: current situation and alternative sources. Springer International publishing, Cham. P. 63-103.

Gbore F A, Adewole AM, Oginni O, Oguntolu MF, Bada AM, Akele O. 2010. Growth performance, haematology and serum biochemistry of African catfish (Clarias gariepinus) fingerlings fed graded levels of dietary fumonisin B1. Mycotoxin Research 26, 221–227.

Gouda A, Amer SA, Gabr S, Tolba SA. 2020. Effect of dietary supplemental ascorbic acid and folic acid on the growth performance, redox status, and immune status of broiler chickens under heat stress. Topical Anima Health Production 1-10.

Han J, Park H, Kim J, Jeong D, Kang J. 2019. Toxic Effects of arsenic on growth, hematological parameters, and plasma components of starry flounder, Platichthys stellatus, at two water temperature conditions. Fisheries and Aquatic Sciences 22, 3.

Hu Y, Huang Y, Wen H, Huan Z, Zhong L, Mao J, Li J, Xiao T. 2013. Effect of vitamin C on growth, immunity and anti-ammonia-nitrite stress ability in juvenile black carp (Mylopharyngodon piceus). Journal of Fish China 37, 565-573.

Huang A, Vita JA, Venema RC, Keaney, JF. 2000. Ascorbic acid enhances endothelial nitric-oxide synthase activity by increasing intracellular tetra-hydrobiopterin. Journal of Biology and Chemistry 275, 17399-17406.

Kadry SM, Marzouk MS, Amer AF, Hanna MI, Azmy AH, Hamed HS. 2012. Vitamin E as antioxidant in female African catfish (Clarias gareipinus) exposed to chronic toxicity of atrazine. Egyptian Journal of Aquatic Biology and Fisheries 16(2), 83-98.

Khalil SR, Reda RM, Awad A. 2017. Efficacy of Spirulina platensis diet supplements on disease resistance and immune-related gene expression in Cyprinus carpio L. exposed to herbicide atrazine. Fish Shellfish Immunology 67, 119–128.

Leduc A, Zatylny-Gaudin C, Robert M, Corre E, Corguille GL, Castel H, Lefevre-Scelles A, Fournier V, Gisbert E, Andree KB, Henry J. 2018. Dietary aquaculture by-product hydrolysates: impact on the transcriptomic response of the intestinal mucosa of European seabass (Dicentrarchus labrax) fed low fish meal diets. BMC Genomics 19, 396.

Liang XP, Li Y, Hou YM, Qui H, Zhou QC. 2017. Effect of dietary vitamin C on the growth performance, antioxidant ability and innate immunity of juvenile yellow catfish (Pelteobagrus fulvidraco Richardson). Aquaculture Research 48(1), 149-160.

Lu SC. 2013. Glutathione synthesis. Biochimical and Biophys Acta 1830, 3143–3153.

Okoye CL, Samuel PO, Arimoro FO, Ayanwale AV, Auta YI, Muhammed AZ. 2018. Effects of lead nitrate on calase production levels in post juvenile Clarias gariepinus (Burchell, 1822). International Journal of Fisheries and Aquaculture 10 (1), 1-7.

Padayatty SJ, Katz A, Wang Y, Eck P, Kwon O, Lee JH, Dutta SK. 2003. Vitamin C as an antioxidant: evaluation of its role in disease prevention. Journal of the American College of Nutrition 22(1), 18-35.

Rajagopalan R, Kode A, Penumatha SV, Kallikat NR, Venugopal PM. 2004. Comparative effects of curcumin and an analog of curcumin on alcohol and PUFA induced oxidative stress.

Romeo M, Bennani N, Gnassia-Barelli M, Lafaurie M, Girard JP. 2015. Cadmium and copper display different response towards oxidative stress in the kidney of the sea Bass Dicentrarchus labrax. Aquatic Toxicology 48, 185-194.

Samuel PO, Abiola OP, Arimoro OF, Ayanwale AV, Muhammed A Z, AutaYI. 2017. Effects of Lead Nitrate on Glutathione Production Levels in Clarias gariepinus (Burchell, 1822) Post Juveniles. Journal of Aquatic Sciences 32 (2), 223-229.

Samuel PO, Arimoro FO, Ayanwale AV, Mohammad HL. 2021. Assessment of the Ameliorative Roles of Vitamins A, C and E on Morphometric Parameters of Clarias Gariepinus (Burchell, 1822) Fingerlings Exposed to Cadmium Chloride. Journal of Agriculture and Aquaculture 3(1), 1-15. DOI: 10.5281/zenodo.4575180.

Stanic B, Andric N, Zoric S, Grubor-Lajsic G, Kovacevic R. 2011. Assessing pollution in the Danube river near Novi Sad (Serbia) using several biomarkers in starlet (Acipenser ruthenus L.). Ecotoxicology and Environmental Safety 65(3), 395-402.

Sumit R, Vincent S, Meena B. 2014. Catalase and Glutathione-S Transferase Activity in Different Tissues of Freshwater Catfish Clarias gariepinus on Exposure to Cadmium. International Journal of Pharmacy and Biological Science 5(1), 963-971.

Sun Y, Ma A, Li Y, Han X, Wang Q, Liang H. 2012. Vitamin E supplementation protects erythrocyte membranes from oxidative stress in healthy Chinese middle-aged and elderly people. Nutrition Research 32, 328-334.

Ukwuani-Ko H, Park H, Kang J. 2019. Change of growth performance, haematological parameters and plasma component by hexavalent chromium exposure in starry flounder, Platichthys stellatus. Fisheries and Aquatic Sciences 22, 9.

Zelko IN, Mariani TJ, Folz RJ. 2002. Superoxide dismutase multigene family: A comparison of the CuZn-SOD (SOD1), Mn-SOD (SOD2) and EC-SOD (SOD3) gene structures, evolution and expression. Free Radical Biological Medicine 33, 337-349.