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Effects of different effluents on the yield quality and heavy metals accumulation in spinach (Spinacia oleracea L.)

Research Paper | December 1, 2017

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Noor-ul-Ain, Ejaz Ahmad Khan, Iqtidar Hussain, Sadaf Javeria, Yasir Ihtesham

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Int. J. Biosci.11( 6), 239-247, December 2017

DOI: http://dx.doi.org/10.12692/ijb/11.6.239-247


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Heavy metals are one of the important types of contaminants that can be found on the surface and in the tissue of fresh vegetables. In this regard, a study was conducted to see the impact of different effluents on the growth, yield and heavy metals content of spinach grown in pots. The plants were treated with 6 different effluents viz. 10% effluents of sugar mill, sewage water, soap factory, ghee mill, slaughterhouse and Control. Data on weight of leaves plant-1 (g), number of leaves plant-1, yield ha-1, Vitamin C and heavy metals (Fe, Pb, Zn, Ni, Cd) were recorded. The results indicated that irrigation with effluents significantly affected all parameters studied. Sewage water, sugar mill effluent and slaughterhouse wastage significantly improved the yield and growth characteristics while ghee mill and soap factory effluents reduced these traits. Irrigation with sewage water increased 11.88% yield while the increment with slaughterhouse wastage and sugar mill effluent was 11.41% and 9.6%. Ghee mill and soap factory effluents reduced 8.9% and 7.38%, respectively. Heavy metal concentrations were increased with all types of effluents. The transfer factors showed that the accumulation of metals was in the order of Pb> Fe >Zn > Cd.


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Effects of different effluents on the yield quality and heavy metals accumulation in spinach (Spinacia oleracea L.)

Adhikari S, Mitra A, Gupta SK, Banerjee SK. 1998. Pollutant metal contents of vegetables irrigated with sewage water. Journal of Indian Society of Soil Science 46, 153-155.

Ale R, JhaPK, Belbase N. 2008. Effects of distillery effluent on some agricultural crops: A case of environmental injustice to local farmers in Khajura, VDC, Blanke. Scientific World 6, 68-75.

Antoniadis V. 1998. Heavy metal availability and mobility in sewage sludge treatment soil. PhD thesis. The University of Reading.

Atta S, Ahmad T, SattarA, Zuha S. 1998. Heavy metal contamination of vegetables grown on sewage and irrigated waters. Pakistan Journal of Food Science 8, 26-29.

Barzegar M, Erfani F, JabbariA, Hassandokht MR. 2007. Chemical composition of 15 Spinach (Spinace aoleracea L.) cultivars grown in Iran. Italian Journal of Food Science 19, 309-318.

Bashir E and Bantel R. 1994. In “Horticulture” National Book Foundation, Islamabad.

Bhandari G. 2014. A review of urban water reuse-limits, benefits and risks in Nepal. International Journal of Geology, Agriculture & Environmental Sciences 2(1), 21-27.

Brar MS, Khurana MPS, Kansal BD. 2002. Effect of irrigation by untreated sewage effluents on the micro and potentially toxic elements in soils and plants. In Proc 17th World Congress of Soil Science held at Bangkok, Thailand from August 14-21, 2002, Volume-IV, Symposium no. 24, 2002, p. 198(1)-198(6). DOI:

Brar MS, Mahli SS, Singh AP, Arora CL, Gill KS. 2000. Sewage water irrigation effects on some potentially toxic trace elements in soil and potato plants in northwestern India. Canadian Journal of Soil Science 80, 465-471.

Chandorkar S, Deota P. 2013. Heavy metal content of foods and health risk assessment in the study population of Vadodara. Current World Environment 8(2), 291-197. DOI: 

Cheraghi CAK, Pathak C. 2013. Enrichment and translocation of heavy metals in soil and Spinacea oleracea grown in sugar mill effluent irrigated soil. Sugar Technology 15(1), 77-83.

Dan EU, UdoUE,Ituen EB. 2013. Comparative assessment of proximate and heavy metal composition in some selected edible vegetable leaves sourced from three major markets in AkwaIbom state, Southern Nigeria. Australian Journal of Basic and Applied Sciences 7(8), 676-682.

Demirezen D, Aksoy A. 2006. Heavy metal levels in vegetables in Turkey are within safe limits for Cu, Zn, Ni and exceeded for Cd and Pb. Journal of Food Quality 29(3), 252-265. DOI: 10.1111/j.1745-4557.2006.00072.x.

Deshm EG, Etuk H, Johnson A. 2007. Heavy metals accumulation by Talinum triangulare grown on Waste Dumpsites in Uyo Metropolis, AkwaIbom State, Nigeria. Journal of Applied Sciences, 7(10): 1404-1409FAO/WHO. 2001. Codex Alimentarius Commission (FAO/WHO). Food additives and cont aminants-Joint FAO/WHO Food Standards Programme. 2001, ALINORM 01/12A pp. 1-289. DOI: jas.2007.1404.1409&org=11.

Farooq MA, Farooq UR. 2008. Appraisal of heavy metals contents in different vegetables grown in the vicinity of an industrial area. Pakistan Journal of Botany 40(5), 2099-2106. DOI:

Harati M, Kelidari A, Rastegar MT. 2012. Effect of urban wastewater on forage maize in south of Tehran. International Research Journal of Applied and Basic Sciences 3(3), 650-658.

Hussain T. 1987. Food composition tables for cereals, vegetables and fruits. Department of Agricultural Chemistry, Faculty of Agriculture, NWFP. Agricultural University, Peshawar.

Kamal RM, Ahmad F, Ali AS, Khan OS, Wahid A, Farhan M, Ahmad M. 2014. Assessment of heavy metals in vegetables cultivated in land irrigated from Hudiara drain. Journal of Environmental Sciences Computer Sciences and Engineering and Technology 3(3), 1218-1227.

Kiziloglu M, Turan M, Sahin U, Angin I, Anapali O,Okuroglu O. 2007. Effects of wastewater on soil, cabbage plant (Brassica olerecea var. capitatecv. Yalovae) chemical properties. Journal of Plant Nutrition and soil Science 170(1), 166-172. DOI: 10.1002/jpln.200621971/full.

Kumar V, Chopra AK. 2013. Distribution, enrichment and accumulation of heavy metals in soil and Trigonella foenumgraecum L. (Fenugreek) after fustigation with paper mill effluent. Open Journal of Metal 3, 8-20.

Mahdavian SE, Somashekar RK. 2008. Heavy metals and safety of fresh fruits in Bangalore city, India-A case study. Kathmandu University Journal of Science Engineering and Technology 17-27.

Mohamad HH, Latif PA. 2010. Uptake of cadmium and zinc from synthetic effluent by water hyacinth (Eichhornia crassipes). Environment Asia 3, 36-42.

Nankishore A. 2014. Heavy metal levels in leafy vegetables from selected markets in Guyana. Journal of Agricultural Technology 10(3), 651-663. DOI: 10.12691/ajfst-2-5-1.

Nawaz A, Khursid K, Arif MS, Ranjha AM. 2006. Accumulation of heavy metals in soil and rice plant (Oryza sativa L.) irrigated with industrial effluents. International Journal of Agriculture and Biology 8(3), 391-393.

Orhue ER, OsiagbovoAU, Vwioko DE. 2005. Growth of maize (Zea mays L.) and changes in some chemical properties of an Ultisol amended with brewery effluent. African Journal of Biotechnology 4, 973-978.

Perveen S, Samad A, Nazif W, Shah S. 2012. Impact of sewage water on vegetables quality with respect to heavy metals in Peshawar, Pakistan. Pakistan Journal of Botany 44(6), 1923-1931.

Powel W, Morgant M, Andre C, Hanafay M, Vogel J, Tingrey S, Rafalski A. 1996. The comparison of RFLP, AFLP, RAPD and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding 21, 225-238.

Ramesh HL, Murthy VNY. 2012. Assessment of heavy metal contamination in green leafy vegetables grown in Bangalore urban district of Karnataka. Advances in Life Science and Technology 6, 40-51.

Sridhara CN, Kamalaa CT, Raj DSS. 2008. Assessing risk of heavy metals from consuming food grown on sewage irrigated soils and food chain transfer, Ecotoxicology and Environmental Safety 69(3), 513-524. DOI: 10.1016/j.ecoenv.2007.04.013.

Steel RGD, Torrie JH, Dickie DA. 1997. Principles and procedures of statistics-a biometric approach. Third edition Mc Graw-Hill Publishing Company U.S. Department of Agriculture, Agricultural Research Service 2012.

Victor RB. 2010. Garden Peas and Spinach from the Middle East. Reprint of “Our Vegetable Travelers”. National Geographic Magazine 96, 2.