Welcome to International Network for Natural Sciences | INNSpub

Effects of Moringa leaf meal (MLM) inclusion as premix on the haematological parameters of Clarias gariepinus

Research Paper | October 3, 2022

| Download 38

KO. Awhefeada, EC. Enujeke, OG. Ikpinima, MC. Umehai

Key Words:

J. Bio. Env. Sci.21( 4), 15-21, October 2022


JBES 2022 [Generate Certificate]


This study was conducted to determine the haematological parameters of Clarias gariepinus juveniles, fed diets with Moringa oleifera leaf meal (MLM) as premix for 8 weeks. Fish mean weight of 12.5±0.04g was used. The experimental diets were formulated at 40% crude protein into three treatments of the same composition, but different inclusion levels of moringa, before processing into pelleted sinking feed. The treatments based on % mLM were 0, 0.5, 1.0 and 1.5. Both floating and pelleted sinking feeds were used. Each diet was fed to a group of thirty (30) juveniles of Clarias gariepinus per treatment at 5% body weight. Each treatment was replicated three times. The results showed that Treatment IV had the best haematological indices; and was not significantly different (P>0.05) from other treatments with respect to packed cell volume (PCV) 27.83±1.23; Haemoglobin (Hb): 8.73±0.81 and Red Blood Cell (RBC): 2.66±0.54. Treatment I equally had good haemotological Parameters: PCV (24.37±2.85); Hb (8.34±0.79); RBC (2.83±0.53), Platelet (123.45±7.81). Treatments II and III had higher levels of White Blood Cell (WBC) which ranged from 17.43±1.235 to 17.61±1.33. Haematological parameters were within the recommended range for fish health. This study revealed that moringa leaf meal (MLM) was best as replacement of premix; for improved performance for the tested parameters of Clariasgariepinus. Based on the findings of the study, it is recommended that 1.5%mLM inclusionrate as premix for enhancement of haematological parameters and better fish health of Clarias gariepinus.


Copyright © 2022
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Effects of Moringa leaf meal (MLM) inclusion as premix on the haematological parameters of Clarias gariepinus

Abdul-Mobdy HE, Abdel-Aal HA, Saizan SL, Nassar AG. 2021. Nutritional value of African Catfish (Clarias gariepinus) meat. Asian Journal of Applied Chemistry Research 8(2), 31-39.

Androw BL. 1972. Experimental physiology.9th ed pp. 1228.

Anwalu BM. 2009. Agronomic management practice of Moringa (Moringa Oleifera Lam).Paper for commercial presented at a two-day workshops on sustainable production and commercialization of Moringa.

Blaxhall PC, Daisley KW. 1973. Routine haematological methods for use changes in Nile Tilapia (Oreochromis niloticus) fed with varying dietary chloride. Pollution Resources 21(2),129-131.

Faheyi JW. 2005. Moringa Oleifera-a review of the medical evidence for its nutritional therapeutics and prophylactic properties part1. Trees for life Journal 1, 5.

Falaye AE, Omoike A, Awhefeada KO. 2018. The haematologica parameters of Catfish (Clarias gariepinus) fed fish feeds with replaced premix using Moringa leaf meal (MLM). Madridge Journal of Aquaculture Research and Development. Madridge Publishers.

Falaye AE. 2013. Fish: A jewel in land and sea environment; An Inaugural lecture, Ibadan University Press pp7-31.

Josh PK, Bose M, Harish D. 2002. Changes in certain haematological parameters in siluroid catfish Clarias batrachus (Linn) exposed to cadmium chloride. Pollution Resources 21(2), 129-131

Kalb Kuo G. 2002. .AVRDC report-2001.Asaian vegetable Research and Development Centre, Shanhuawa, Tainan, Taiwan.

Kang, Ombe J, Likongwe JS, Eda H, Mtimuni JP. 2007. Effects of varying dietary energy level on feed intake, feed conversion, whole body composition and growth of Malawian tilapia, Oreochromis shiranus Boulenger. Aquaculture research 38, 373-380.

Keri-Alhadi I, Aziz Bin A, Abol-Munati AB. 2012. Haematological lecture, Ibadan University Press pp. 7-31.

Merghami TH. 2010. The core of medical physiology 3rd edition pp.156-158.

Mohamed E, Abd El-Hack, Mahmoud Alagawany, Ahmed SM, Elaahal, Shaaban S, Elnesr, Ayman, Swelum A. 2018. Effects of forage Moringa oleifera L. (moringa) on Animal health and nutrition and its beneficial application in Soil, Plants and water purification. Agriculture review 8(145), 1-22. MDPI Publisher.

Montagne L, Pluke JR, Hampson DJ. 2003. A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Animal Feed Science and Technology 108(1), 95-117. DOI: 10.1016/S0377-8401(03)00163-914.

Nelson JS, Grande TC, Wilson MVH. 2016..Fisher of the World . Animal Science and Zoology.John Wiley and Sons Inc.5th Edition. (PDF) The Hematological parameters of Catfish (Clarias gariepinus) fed Fish Feeds with replaced Premix using Moringa Leaf Meal (MLM).

Osuigwe DI, Nwosu C, Ogunji JO. 2005. Preliminary Observation on someheamatological parameters of juvenile Heterobr anc huslong filis fed different dietary levels of raw and boiled jack bean (Canavalia ensiformis) seed meal. Conference on International Agricultural Research for Development 24(2), 19-26. doi:10.4314/nvj.v24i2.345015

Ozovehe BN. 2013. Growth performance, haematological indices and some biochemical enzymes of juveniles Clarias gariepinus (Burchell 1822) fed varying levels of Moringa oleifera leaf meal diet. J. Aquacult. Res. Dev 4(2), 166.

Palademc, Chang LC. 2003. Suggested cultural practice for Moringa. International Cooperative guide. Asian Vegetable Research and Development Centre 3-545 pp.

Sotolu AO, Faturoti EO. 2011. Growth Performance And Hematological Effects Of Varying Dietary Processed Leucaena Leucocephala Seed Meal In Clarias gariepinus (Burchell, 1822) Juveniles. African Journal of Food, Agriculture, Nutrition and Development II(I), 4547-4557.