Effects of Moringa oleifera leaf meal (MOLM) based diets on carcass composition and hematology of Labeo rohita fingerlings

Paper Details

Research Paper 01/07/2016
Views (359) Download (30)
current_issue_feature_image
publication_file

Effects of Moringa oleifera leaf meal (MOLM) based diets on carcass composition and hematology of Labeo rohita fingerlings

Muhammad Zubair-Ul-Hassan Arsalan, Syed Makhdoom Hussain, Muhammad Asrar, Haseeb Anwar, Muhammad Masoom-Ul-Hassan Rehan, Muhammad Mudassar Shahzad, Danish Riaz, Nisar Ahmad, Naveeda Wahab
J. Bio. Env. Sci.9( 1), 214-223, July 2016.
Certificate: JBES 2016 [Generate Certificate]

Abstract

A 70 – day feeding trial was carried out to study the effect of replacing the dietary fish meal with Moringa oleifera leaves meal (MOLM) on the carcass composition and hematological indices of Labeo rohita fingerlings. Five iso-nitrogenous diets were formulated with MOLM replacing 0%, 10%, 20%, 30% and 40% of fish meal in the diets. Fish were fed at 5% of their body weight two times daily. Fingerlings (avg. wt. 6.61±0.053g) were randomly distributed into tanks having 15 fish in each replicate. Results from proximate analysis showed that replacement of fish meal with MOLM upto 10% increased crude protein and crude fat in fish body as compared to fish fed on control (0%), 20%, 30% and 40% MOLM based diet, respectively. The hematological parameters of fingerlings fed 10% MOLM based diet were found to be significantly different (p<0.05) from the fish fed control diet. The red blood cells and hemoglobin of fish showed a significant (P<0.05) inverse correlation with increase in MOLM in diets. The present study showed that MOLM has good prospective for use as fish meal replacement in L. rohita diet up to 10% level without compromising fish performance whereas the hematological investigation indicated that inclusion of MOLM above 20% in the diets showed the hematological disturbance. On the base of this study it was concluded that fish meal can be replaced with MOLM up to 10% in the diets to increase the nutritive values of L. rohita fingerlings.

VIEWS 44

Adedeji OB, Taiwo O, Agbede SA. 2000. Comparative haematology of five Nigerian freshwater fish species. Nigerian Veterinary Journal 21, 75-84.

Aderolu AZ, Aarode OO, Akonu AA, Jimoh WA. 2011. The effect of substituting maize with graded level of biscuit waste on growth, nutrient utilization, carcass composition, haematological parameter and economic performance of African catfish (Clarias gariepinus). Journal of Natural Science, Engineering and Technology 10, 111-120.

Allan GL, Rowland SJ. 1992. Development of an experimental diet for silver perch (Bidyanus bidyanus). Austasia Aquaculture 3, 39-40.

Anyanwu DC, Udedibie ABI, Osuigwe DI, Ogwo VO. 2011. Haematological responses of hybid of Heterobranchus bidorsalis x Clarias gariepinus fed dietary levels of Carica papaya leaf meal. World Rural Observation 3, 9-12.

AOAC (Association of Official Analytical Chemists) (1995). Official Methods of Analysis. 15th Ed. Association of Official Analytical chemists, Washington, D.C. USA., p. 1094.

Ayotunde EO, Offem BO, Bekeh AF. 2011. Toxicity of Carica papaya Linn: Haematological and Piscicidal Effect on Adult Catfish (Clarias gariepinus). J. of Fisheries and Aquatic Science 6, 291–308.

Bello NO, Nzeh GC. 2013. Effects of varying levels of Moringa oleifera leaf meal diet on growth performance, hematological indices and biochemical enzymes of African catfish Clarias gariepinus (Burchell 1822). Elixir Aquaculture A 18, 14459-14466.

Blaxhall PC, Daisley KW. 1973. Routine haematological methods for use with fish blood. Journal of Fish Biology 6, 771-781. 10.

Cao L, Wang W, Yang C, Yang Y, Diana J, Yakupitiyage A, Luo Z, Li D. 2007. Application of microbial phytase in fish feed. Enzyme Microbiology and Technology 40, 497-507.

Dahi IJ, Bjorkkjar IE, Graft Makia MK, Idemensten B. 2006. Fish more than just Omega 3. Tidsskrift for Den norske legeforening 126, 309-311.

Dienye HE, Olumuji OK. 2014. Growth performance and haematological responses of African mud catfish Clarias gariepinus fed dietary levels of Moringa oleifera leaf meal. Net Journal of Agricultural Science 2, 79-88.

Douglass JW, Janes KW. 2010. In: Schalm’s Veterinary Haematology. John Wiley and Sons. Blackwell publishing Limited 1232.

Eyo AA. 2000. Fish processing Technology in the tropics. University of Ilorin, Nigeria 430 pp.

FAO. (Food and agriculture organization) 2006. Fishery statistics (Aquaculture production). Food and agriculture organization of the United Nations, 99.

Francis G, Makkar HPS, Becker K. 2002. Anti-nutritional factors present in plant derived alternative fish feed ingredients and their effects in fish. Aquaculture 199, 197–227.

Ganzon-Naret ES. 2014. Utilization of Moringa oleifera leaf meals as plant protein sources at different inclusion levels in fish meal based diets fed to Lates calcarifer. ABAH Bioflux 6, 158-167.

Gokcek CK, Mazlum Y, Akyurt I. 2008. Effect of Feeding Frequency on the Growth and Survival of Himri Barbel Barbus luteus (Heckel, 1843), Fry under Laboratory Conditions. Pakistan Journal of Nutrition 7, 66-69.

Hrubec TC, Cardinale JL, Smith SA, 2000. Haematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hybrid). Veterinary Clinical Pathology 29, 7-12.

Hussain SM, Afzal M, Javid A, Hussain AI, Ali Q, Mustafa I, Chatha SAS, Shah SZH, Hussain M, Ullah MI. 2015. Efficacy of phytase supplementation on growth performance and mineral digestibility of Labeo rohita fingerlings fed on Cottonseed meal based diet. Pakistan Journal of Zoology 47, 699-709.

Lech PL, Reigh RC. 2012. Plant products affect growth and digestive efficiency of cultured Florida pompano (Trachinotus carolinus) fed compounded diets. Open access peer-reviewed scientific journal 7, 1-11.

Lenfant C, Johansen K. 1972. Gas exchange in gill, skin and lung breathing. Respiration Physiology 14, 211–218.

Lovell RT. 1989. Nutrition and Feeding of Fish. Van Nostrand-Reinhold, New York, book 260.

Madalla N, Agbo NW, Jauncey K. 2013. Evaluation of Aqueous Extracted Moringa Leaf Meal as a Protein Source for Nile Tilapia Juveniles. Tanzania Journal of Agricultural Sciences 12, 53-64.

Makkar HPS, Becker K. 1996. Nutritional value and antinutritional components of whole and ethanol extracted Moringa oleifera leaves. Animal Feed Science and Technology 63, 211-228.

Martin L P. 2007. The moringa tree. Echo. North Fort Myers, FL 33917, USA. Available at: http://www.echonet.org/ (Accessed 24 December 2013).

Odedeyi DO. 2014. Nutritive Values of The Carcass Of African Catfish (Clarias Gariepinus) Fingerlings Fed Raw And Steam-Heated Moringa oleifera diets, Advances in Environment, Agriculture & Medical Sciences (ICAEAM’14) Kuala Lumpur (Malaysia) iaast.org/upload/6846A1114041

Ojewole GS, Ammah SI. 2006. Nutritive and economic value of Danish Fish meal, crayfish, dustmeal and shrimp waste meal, inclusion in broiler diet, International Journal of Science 5, 390-394.

Olaniyi CO, Ajani NO, Adetomi MN. 2013. Growth performance and nutrient utilization of Clarias gariepinus fed Moringa oleifera leaf meal. Journal of Natural Sciences Research 3, 99-104.

Osuigwe DI, Obiekezie AI, Onuoha GC. 2005. Some haematological changes in hybrid catfish (Heterobranchus longifilis x Clarias gariepinus) fed different dietary levels of raw and boiled jackbean (Canavalia ensiformis) seed meal. African Journal of Biotechnology 4, 1017-1021.

Oyawoye EO, Ogunkunle M. 1998. Physiological and biochemical effects of raw jack beans on broilers. Proceedings of annual Conference of Nigerian Society of Animal production 23, 141-142.

Pham MA, Lee KJ, Dang TM, Lim SJ, Ko GY, EO J, Oh DH. 2008. Improved apparent digestibility coefficient of protein and phosphorus by supplementation of microbial phytase in diets containing cottonseed and soybean meal for juvenile olive flounder. Asian-Australasian Journal of Animal Science 21, 1367-1375.

Ping Z, Wenbing Z, Davis DA, Chhorn L. 2010. Growth Response and Feed Utilization of Juvenile Hybrid Catfish Fed Diets Containing Distiller’s Dried Grains with Solubles to Replace a Combination of Soybean Meal and Corn Meal. North American Journal of Aquaculture 72, 298-303.

Rainza-paiva MJT, Ishikawa CM, Das-Eiras AA, Felizardo NN. 2000. Haematological analysis of chara Pseudoplatystoma fasciatum in captivity. Aqua 2000. Responsible aquaculture in the new millennium. Nice, France. European Aquaculture Society Special Publication 28, 590.

Rowland SJ, Ingram BA. 1991. Diseases of Australian native fishes. In: Fisheries Bulletin 4 NSW Fisheries, Sydney, NSW, Australia.

Snedecor GW, Cochran WG. 1991. Statistical Methods. 8th Ed. Iowa State University. Press, Americans.USA, 503.

Soliva CR, Kreuzer M, Foidl N, Foidl G, Machmuller A, Hess HD. 2005. Feeding value of whole and extracted Moringa oleifera leaves for ruminants and their effects on ruminal fermentation in vitro. Animal Feed Science and Technology 118, 47-62.

Steel RGD, Torrie JH, Dickey DA. 1996. Principles and procedures of statistics, 3rd Ed. McGraw Hill international Book Co. Inc., New York. USA 336-352.

Steften W. 2006. Fresh water fish wholesome foodstuff. Journal of Agricultural Science 320-328.

Thiam S, Fall J, Loum A, Sagne M, Diouf M. 2015. Use of Effective Microorganisms (Em) in Tilapia Diets: Effects of Growth Performance and Carcass Composition. International Journal Current Microbiology Applied Science 4, 536-49.

Wedemeyer GA, Yastuke WT. 1977. Clinical methods for the assessment of the effects of environmental stress on fish health. United States Fish and Wildlife Service Technical papers 89.

Wgner EJ, Jensen T, Arndt R, Routledge, MD, Brodwich Q. 1997. Effects of rearing density upon cutthroat hematology, hatchery performance, fin erosion and general health and condition. The Progressive Fish-Culturist 59, 173-187.

Zhou QC, Tan BP, Mai KS, Liu YJ. 2004. Apparent digestibility of selected feed ingredients for juvenile cobia Rachycentron canadum. Aquaculture 241, 441-451.