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Effects of Murashige and Skoog medium strength on germination and secondary metabolites production of Eleutherococcus senticosus’s somatic embryos in bioreactor

Abdullah Mohammad Shohael, Sayeda Mahfuja Khatun, Mohammad Firoz Alam, Kee Yoeup Paek

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Int. J. Biosci.3(3), 155-163, March 2013

DOI: http://dx.doi.org/10.12692/ijb/3.3.155-163

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Abstract

Somatic embryos of Eleutherococcus senticosus were germinated into different strength of Murashige and Skoog medium (MS) in balloon type bubble bioreactor (working volume 2Lit) to investigate the effects of medium strength on biomass growth and metabolites production. Fresh weight, dry weight and % dry weight of the germinated somatic embryos increased with the increase of medium strength up to MS2X. Further increase in medium strength decreased all the growth parameters mentioned above significantly. Maximum amount of total eleutheroside (166.37µg/g DW) was obtained in full strength of MS medium but highest chlorogenic acid (2.26 mg/g DW) was obtained in MS2X. Contents of total phenolics and flavonoids were increased with the decrease of MS strength but polysaccharides content increased when medium strength increased. Thus it may explore immense practical applicability in somatic embryos scale up, where the secondary metabolite production from germinated somatic embryos is directly related to MS medium strength.

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Effects of Murashige and Skoog medium strength on germination and secondary metabolites production of Eleutherococcus senticosus’s somatic embryos in bioreactor

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