Effects of nano-iron spraying on the antioxidant activities of canola leaf under drought stress

Paper Details

Research Paper 01/07/2017
Views (283) Download (12)

Effects of nano-iron spraying on the antioxidant activities of canola leaf under drought stress

Reza Shokri-Gharelo, Morteza Derakhti-Dizaji, Ghader Rostami
J. Bio. Env. Sci.11( 1), 304-311, July 2017.
Certificate: JBES 2017 [Generate Certificate]


Drought stress limits plant productivity and performance constraining safe food production worldwide. Reactive oxygen species (ROS) are mostly harmful event taking place in plants under abiotic stresses. This study aimed to evaluate nano-iron foliar application in reducing ROS damage through measuring electrolyte leakage, total phenolic content, antioxidant enzymes (ascorbate peroxidase, catalase, and guaiacol peroxidase), and antioxidant activity in canola under 30% FC of drought stress. Results indicated that the effect of drought stress and nano-iron spraying were significant on studied parameters (p<0.01). The plants treated with nano-iron compared to untreated plants showed remarkably a low electrolyte leakage and high total phenolic content, antioxidant enzyme activities, and antioxidant activity under 30% FC (LSD<0.05). In conclusion, it is suggested that nano-iron is effective in reducing ROS damage and improving oxidative defense system in canola.


Al-Amier H, Craker LE. 2007. In-vitro selection for stress tolerant spearmint. Issues in New Crops and New Uses 306-310.

Bandehagh A, Salekdeh GH, Toorchi M, Mohammadi A, Komatsu S. 2011. Comparative proteomic analysis of canola leaves under salinity stress. Proteomics 11, 1965-1975.

Bian S, Jiang Y. 2009. Reactive oxygen species, antioxidant enzyme activities and gene expression patterns in leaves and roots of Kentucky bluegrass in response to drought stress and recovery. Scientia Horticulturae 120, 264-270.

Bor M, Özdemir F, Türkan I. 2003. The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Science 164, 77-84.

Cakir R. 2004. Effect of water stress at different development stages on vegetative and reproductive growth of corn. Field Crops Research 89, 1-16.

Chance B, Maehly A. 1955. [136] Assay of catalases and peroxidases. Methods in enzymology 2, 764-775.

Cruz de Carvalho MH. 2008. Drought stress and reactive oxygen species: production, scavenging and signaling. Plant signaling & behavior 3, 156-165.

El-Feky SA, Mohammed MA, Khater MS, Osman YA, Elsherbini E. 2013. Effect of magnetite Nano-Fertilizer on Growth and yield of Ocimum basilicum L. Int. J. Indigenous Med. Plants 46, 1286-1293.

El-Sherbini E, El-Gawad HA, Kamal M, El-feky SA. 2015. Potential of He-Ne laser irradiation and iron nanoparticles to increase growth and yield of pea. Nanotechnology 3, 4.

Fan X, Sokorai KJ. 2005. Assessment of radiation sensitivity of fresh-cut vegetables using electrolyte leakage measurement. Postharvest biology and technology 36, 191-197.

Farooq M, Hussain M, Wahid A, Siddique K. 2012. Drought stress in plants: an overview. Plant responses to drought stress. Springer p. 1-33.

Gharelo RS. 2016. Determination of Metabolic pathways and PPI network of Sarigol in Response to Osmotic stress: An in silico study. International Journal for Computational Biology (IJCB) 5, 21-27.

Gharelo Shokri R, Bandehagh A, Tourchi M, Farajzadeh D. 2016. Canola 2-dimensional proteom profile under osmotic stress and inoculation with Pseudomonas fluorescens FY32. Plant Cell Biotechnology and Molecular Biology 17, 257-266.

Gill SS, Tuteja N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant physiology and biochemistry 48, 909-930.

Hu Y, Schmidhalter U. 2005. Drought and salinity: a comparison of their effects on mineral nutrition of plants. Journal of Plant Nutrition and Soil Science 168, 541-549.

Kapoor D, Sharma R, Handa N, Kaur H, Rattan A, Yadav P, Gautam V, Kaur R, Bhardwaj R. 2015. Redox homeostasis in plants under abiotic stress: role of electron carriers, energy metabolism mediators and proteinaceous thiols. Frontiers in Environmental Science 3, 13.

Lutts S, Kinet J, Bouharmont J. 1996. NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany 78, 389-398.

McCollum T, McDonald R. 1991. Electrolyte leakage, respiration, and ethylene production as indices of chilling injury in grapefruit. HortScience 26, 1191-1192.

Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in plant science 7, 405-410.

Mittler R, Vanderauwera S, Gollery M, Van Breusegem F. 2004. Reactive oxygen gene network of plants. Trends in plant science 9, 490-498.

Mittova V, Tal M, Volokita M, Guy M. 2002. Salt stress induces up‐regulation of an efficient chloroplast antioxidant system in the salt‐tolerant wild tomato species Lycopersicon pennellii but not in the cultivated species. Physiologia Plantarum 115, 393-400.

Moon J-H, Terao J. 1998. Antioxidant activity of caffeic acid and dihydrocaffeic acid in lard and human low-density lipoprotein. Journal of Agricultural and Food Chemistry 46, 5062-5065.

Nakano Y, Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and cell physiology 22, 867-880.

Nasiri Y, Zehtab-Salmasi S, Nasrullahzadeh S, Najafi N, Ghassemi-Golezani K. 2010. Effects of foliar application of micronutrients (Fe and Zn) on flower yield and essential oil of chamomile (Matricaria chamomilla L.). Journal of Medicinal Plants Research 4, 1733-1737.

Noctor G, Foyer CH. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annual review of plant biology 49, 249-279.

Peever TL, Higgins VJ. 1989. Electrolyte leakage, lipoxygenase, and lipid peroxidation induced in tomato leaf tissue by specific and nonspecific elicitors from Cladosporium fulvum. Plant Physiology 90, 867-875.

Plewa MJ, Smith SR, Wagner ED. 1991. Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutation research/fundamental and molecular mechanisms of mutagenesis 247, 57-64.

Purty RS, Kumar G, Singla-Pareek SL, Pareek A. 2008. Towards salinity tolerance in Brassica: an overview. Physiology and Molecular Biology of Plants 14, 39-49.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine 26, 1231-1237.

Said-Al Ahl H, Mahmoud AA. 2010. Effect of zinc and/or iron foliar application on growth and essential oil of sweet basil (Ocimum basilicum L.) under salt stress. Ozean Journal of Applied Sciences 3, 97-111.

Sairam R, Srivastava G. 2002. Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress. Plant Science 162, 897-904.

Sairam R, Srivastava G, Agarwal S, Meena R. 2005. Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes. Biologia Plantarum 49, 85-91.

Scott N, Chen, H. 2013. Nanoscale science and engineering for agriculture and food systems. Industrial Biotechnology 9, 17-18.

Shi H, Jiang C, Ye T, Tan D-x, Reiter RJ, Zhang H, Liu R, Chan Z. 2015. Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of improved abiotic stress resistance in bermudagrass [Cynodon dactylon (L). Pers.] by exogenous melatonin. Journal of experimental botany 66, 681-694.

Shokri-Gharelo R, Derakhti-Dizaji M, Noparvar P. 2016. Identification of Putative Osmotic Stress-Responsive Genes in Canola by in Silico Study of Cis-Regulatory Elements. Austin J Comput Biol Bioinform 3, 1016.

Singleton V, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture 16, 144-158.

Soltani A, Gholipoor M, Zeinali E. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany 55, 195-200.

Theil EC. 1987. Ferritin: structure, gene regulation, and cellular function in animals, plants, and microorganisms. Annual review of biochemistry 56, 289-315.

Uzilday B, Turkan I, Sekmen AH, Ozgur R, Karakaya H. 2012. Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C 4) and Cleome spinosa (C 3) under drought stress. Plant Science 182, 59-70.

Willekens H, Inzé D, Van Montagu M., Van Camp W. 1995. Catalases in plants. Molecular Breeding 1, 207-228.

Zarch, MAA, Sivakumar B, Sharma A. 2015. Droughts in a warming climate: A global assessment of Standardized precipitation index (SPI) and Reconnaissance drought index (RDI). Journal of Hydrology 526, 183-195.

Zayed B, Salem A, El Sharkawy H. 2011. Effect of different micronutrient treatments on rice (Oriza sativa L.) growth and yield under saline soil conditions. World J. Agric. Sci 7, 179-184.

Zhu Z, Wei G, Li J, Qian Q, Yu J. 2004. Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.). Plant Science 167, 527-533.