Effects of rhizobia inoculation and molybdenum application on nodulation, N uptake and yield of peanut (Arachis hypogaea L.)

Paper Details

Research Paper 01/07/2017
Views (264) Download (13)

Effects of rhizobia inoculation and molybdenum application on nodulation, N uptake and yield of peanut (Arachis hypogaea L.)

Marie Solange Mandou, Asafor Henry Chotangui, Laurette Ngo Nkot, Dieudonné Nwaga
Int. J. Agron. Agri. Res.11( 1), 103-113, July 2017.
Certificate: IJAAR 2017 [Generate Certificate]


A field experiment was conducted in two sites of the humid forest zone of the Centre Region of Cameroon ( (Yaounde and Bokito), to study the interactive effect of rhizobia inoculation and Molybdenum (Mo) application on nodulation and yield of peanut (Arachis hypogaea L.). The experiment was laid out in a randomized complete block design with four replications. The treatments consisted of control (C), Rhizobium inoculation (R), Mo application (Mo) and a combination R+Mo. The results showed that Molybdenum and rhizobia inoculation had a significant effect on the yield of peanut at both sites. The inoculation with rhizobia showed a remarkable effect on the nodulation of the fallow of Yaounde (67. 73%) and not on the mixed- farm in Bokito (55. 43%) at P = 0.05. Nodulation was also stimulated through the combination of rhizobia inoculation with molybdenum application with increment of 111.26% and 50.44% nodule dry weight respectively for Yaounde and Bokito sites. The application of molybdenum alone improved significantly (P = 0.05) seed yield of peanut in both sites. However the increment was high in Yaoundé site (174%) as compared to Bokito site (10%). It stands out clearly that rhizobia inoculation combined with molybdenum application greatly enhanced biological N-fixation of peanut. The effect of these treatments on yield and the nitrogen uptake of peanut were much more remarkable on the fallow of Yaounde than the mixed-farm of bokito. It is therefore possible to greatly stimulate the biological N-fixation and the nitrogen uptake of peanut through symbiosis with the native rhizobia of the soil alone or after inoculation.


Abera T, Semu E,  Debele T, Wegary D, Kim H. 2015. Determination Soil Rhizobium Populations, Intrinsic Antibiotic Resistance, Nodulation and Seed Yield of Faba Bean and Soybean in Western Ethiopia. World Journal of Agricultural Science 11 (5), 311-324.

Alam F, Kim TY, Kim SY, Alam SS, Pramanik P, Kim PJ, Lee YB. 2015. Effect of molybdenum on nodulation, plant yield and nitrogen uptake in hairy vetch (Vicia villosa Roth), Soil Science and Plant Nutrition 61(4), 664-675.

Alwi N, Wynne JO, Schneeweis TJ, Elkana, GE. 1989. Symbiotic relationship between Bradyrhizobium strain and peanut. Crop Science 29, 50-54.

AOAC. 1984. Official methods of analysis. Assoc. Off. Anal. Chem. Inc., USA, p 12-27.

Atemkeng MF, Begoude BAD. 2014. Assessment of indigenous Bradyrhizobia spp population levels in low phosphorus soils of southern Cameroon using cowpea (Vigna unguiculata L. Walp) as trap crop. Journal of Ecology and the Natural Environment 8(8), 280-286.tt.

Bado BV. 2002. Rôle des légumineuses sur la fertilité des sols ferrugineux tropicaux des zones guinéennes et soudaniennes du Burkina Faso. Thèse de Doctorat PhD, Unniversité Laval. 184 p.

Bailey R, Laidlaw LR. 1999. The interactive effects of P, K, lime and molybdenum on the growth and morphology of white clover (Trifolium repens L.) at establishment. Grass and Forage Science 16, 69-76.

Bambara S, Ndakidemi PA. 2010. The potential roles of lime and molybdenum on the growth, nitrogen fixation and assimilation of metabolites in nodulated legume: A special reference to Phaseolus vulgaris L. African journal of biotechnology 8(17), 2482-2489.

Baraibar A, Frioni L, Guedes M.E, Giller KE. 2003. Nodulation of tree legumes and the ecology of their native rhizobial populations in tropical soils. Applied Soil Ecology 22, 211-223.

Bationo A, Mokwunye AU. 1991. Alleviating soil fertility constraints to increased crop production in West Africa: The experience of the Sahel. In: Mokwunye A. Ed, Alleviating soil fertility constraints to increased crop production in West Africa. Kluwer Academic Publishers, Dordrecht, 195-215 P.

Bhuiyan MMH, Rahman MM, Afroze F, Sutradhar GNC, Bhuiyan MSI. 2008. Effect of Phosphorus, Molybdenum and Rhizobium Inoculation on Growth and Nodulation of Mungbean. Journal of Soil and Nature. 2(2), 25-30.

Bogino P, Banchio E, Rinodi G, Bonfiglio C, Giordano W. 2006. Peanut (Arachis hypogaea) response to inoculation with Bradyrhizobium sp, in soils of Argebtina. Annals of Applied Biology 148, 207-212.

Bordeleau LM, Prévost D. 1994. Nodulation and nitrogen fixation in exterme environments. Plant and soil 161, 115-125.

Castro S, Permigiani M, Vinocur M, Fabra A. 1999. Nodulation in peanut (Arachis hypogaea L.) root in the presence of native and inoculated rhizobia strains. Applied soil ecology 13, 39-44.

Dakora F. 1985. Use of intrinsic antibiotic resistance for characterization and identification of rhizobia from nodules of Vigna unguiculata (L.) Walp. and Phaseolus vulgaris (L.). Acta Microbiologica 34, 187‑196.

Date RA. 2000. Inoculated legumes in cropping systems of the tropics. Field Crop Research. 65, 123–136.

El-Azouni IM, Hussein Y,  Shaaban LD. 2008. The associative effect of VA mycorrhizae with Bradyrhizobium as biofertilizers on growth and nutrient uptake of Arachis hypogaea. Research Journal of Agriculture and Biological Sciences 4, 187-197.

FAOSTAT. 2014. www.fao.org/faostat/en

Franco AA, Munns DN. 1981. Response of Phaseolus vulgaris L. to molybdenum under acid conditions. Soil Science Society of American Journal 45, 1144–1148.

Giller KE. 2001. Nitrogen fixation in tropical cropping systems, 2nd Edition. CAB, 140-168 P.

Graham PH, Hungria, M, Tlusty B. 2004. Breeding for better nitrogen fixation in grain legumes: where do the rhizobia fit in? Online. Crop Managementhttp://dx.doi.org/10.1094/CM-2004-0301-02-RV

Houngnandan P, Sanginga N, Woomer P, Vanlauwe B, Van Cleemput O. 2000. Response of Mucuna puriens to symbiotic nitrogen fixation by rhizobia following inoculation in farmers’ fields in the derived savanna of Benin. Biology and Fertility of Soils 30, 558–565.

Hussein J. 2000. Module 1. Soils: its formation and properties. University College of distance education, Harare, Zimbabwe. 26 p.

Jabbar BKA, Saud HM. 2012. Effects of Molybdenum on biological nitrogen fixation by combination of Rhizobium and Azospirillium in soybean under drip irrigation system. Intional Journal of Life Science and Pharma Research 1, 63–37.

Jamal A, Moon YS, Abdin MZ. 2010. Enzyme activity assessment of peanut (Arachis hypogaea L.) under slow-release sulphur fertilization. Australian Journal of Crop science 4(3), 169-174.

Jensen ES, Peoples MB, Boddey RM, Gresshoff PM, Hauggaard-Nielsen H, Alves BJR, Morrison MJ. 2012. Legumes for mitigation of climate change and feedstock in a biobased economy: A review. Agronomy for Sustainable Development 32, 329-364.

Kabir R, Yeasmin S, Akmm I, Mar S. 2013. Effect of Phosphorus, Calcium and Boron on the Growth and Yield of Groundnut (Arachis hypogaea L.) International Journal of Bioscience and Biotechnology 5(3), 51-58.

Lanier JE, Jordan DL, Spears JF, Wells R, Dewayne JP. 2005. Peanut response to inoculation and nitrogen fertilizer. Agronomy Journal 97, 79-84.

Liebenberg AJ. 2002. Dry bean Production. Printed and published by Department of Agriculture, Resource Centre, Directorate Agricultural Information Services, South Africa. p. 27. http://www.nda.agric.za/docs/drybeans2.pdf

Mathu S, Herrmann L, Pypers P, Matiru V, Mwirichia R, Lesueur D. 2012. Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculata L. walp.) and green gram (Vigna radiate L. wilczek.) yields in Kenya. Soil Science and Plant Nutrition 58, 750–763.

Mfilinge A, Mtei K, Ndakidemi PA. 2014. Effects of rhizobium inoculation and supplementation with P and K, on growth, leaf chlorophyll content and nitrogen fixation of bush bean varieties. American Journal of Research Communication 2(10), 49- 87.

Nkot NL. 2009. Acidité du sol comme facteur limitant la fixation de l’azote atmospherique par l’arachide (Arachis hypogaea) en zone de forêt dense humide. Thèse de Doctorat, Université de Yaoundé I, 187 p.

Nicolas MF, Hungria M, Arias CAA. 2006. Identification of quantitative trait loci controlling nodulation and shoot mass in progenies from two Brazilian soybean cultivars. Field Crop Research 95, 355–366.

Ntoukam G, Endondo C, Ousman B, Mekontchou T, Hamasselbe A, Njomata C, Ndikawa R, Abba A. 1996. Production des légumineuses à graines: acquis de la recherché. In : Boukar LS, poulain JF,  Faure G, Ed. Agriculture des savanes du nord Cameroun: vers un developpement solidaire des savanes d’Afrique centrale. CIRAD-CA Garoua-Cameroun, 327-335 P.

Nwaga D, Jansa J Abossolo AM, Frossard E. 2010. The potential of soil beneficial micro-organisms for slash-and-burn agriculture in the humid forest zone of Sub Saharan African. In: Dion P, Ed. Soil Biology and Agriculture in the Tropics. Soil Biology, vol 21. Springer, Berlin, Heidelberg, 81-107 P.

O’hara GW, Boonkerd N, Dilworth MJ. 1988. Mineral constraints to nitrogen fixation. Plant and Soil 108(1), 93-110.

Paudyal SP, Arial RR, Chauhan SVS, Maheshwari DK. 2007. Effect of heavy metals on growth of rhizobium strains and symbiotic efficiency of two species of tropical legumes. Scientific World 5(6), 26-33.

Quaggio JA, Gallo BP Owino-Gerroh C, Abreu MF, Cantarella H. 2004. Peanut response to lime and molybdenum application in low pH soils. Revista Brasileira de Ciencia do solo 28, 659-664.

Rahman MMH, Sutradhar GCC, Rahman MM, Paul AK. 2008. Effect of phosphorus, molybdenum and rhizobium inoculation on yield attributes of mungbean. International journal of Sustainable Crop Production 3(6), 26-33.

Reddy PM, Ladha JK, So R, Hernandez RJ, Ramos MC, Angels OR, Dazzo FB, de Bruijn FJ. 1997. Rhizobial communication with rice roots: induction of phenotypic change, mode of invasion and extent of colonization Plant Soil 194, 81-98.

Sable S,  Sontakey PY, Tagade R, Deotale RD, Manapure P. 1998. Effect of Rhizobium and molybdenum on quality aspects, yield and yield contributing characters of soybean. Journal of Soils and Crops 8(2), 157 – 159.

Sharma MS, Upadhyay MS, Tomar SS. 1988. Water use efficiency of some rainfed crop on a Vertisol as influenced by soil micronutrients and straw mulching. Indian Journal of Soil Science 33, 387-390.

Shil NC, Noor S, Hossain MA. 2007. Effects of boron and molybdenum on the yield of chickpea. Journal of Agriculture and Rural Development 5, 17-24.

Stoorvogel JJ, Smaling EMA, Janssen BH. 1993. Calculating soil nutrient balances in Africa at different scales. Supra-national scale. Fertilizer Research 35, 227-335.

The C. 2000. Identification of heterotic groups for acids soil on some maize varieties in Cameroon. INCO 1 and 2 Meeting, June 2000. Yaoundé, Cameroon.

Somasegaran P, Hoben HJ. 1994. Handbook for Rhizobia: Methods in Legume Rhizobium Technology, Springer Verlag, New York, 58-64 P. 

Thies, JE, Singleton PW, Bohlool BB. 1991. Influence of the size of indigenous rhizobial populations on establishment and symbiotic performance of introduced rhizobia on the field grown legumes. Applied and Environmental microbiology 57, 19-28.

Thies JE, Singleton PW, Bohlool BB. 1995. Phenology, growth, and yield of field-grown soybean and bush bean as a function of varying modes of N nutrition. Soil Biology and Biochemistry 27, 575–583.

Ulzen J, Abaidoo RC, Mensah NE, Masso C, Gadir AH. 2016. Bradyrhizobium inoculants enhance grain yields of soybean and cowpea in Northern Ghana. Frontiers in Plant Science 7, 1770. http://dx.doi.org/10.3389/fpls.2016.01770.

Vieira RF, Vieira C, Cardoso EJBN, Mosquim, PR. 1995. Foliar application of molybdenum in common beans. IV. Remobilization of nitrogen to the reproductive plant parts. In: International Symposium on Sustainable Agriculture for the Tropic: the role of biological nitrogen fixation. 26th Vov.- ist Dec. 1995- Angradod Reis-Rio de Janeiro Brazil, p. 195-196.

Vincent JM. 1970. A Manual for the Practical Study of the Root-nodule Bacteria. International Biological programme Handbook No.15. Blackwell Scientific Publications, Ltd., Oxford, UK.  p. 164.

Zahran HH. 1999. Rhizobium-legume symbiosis and nitrogen fixation under severe conditions and in arid climate. Microbiology and molecular biology Review 63(4), 968-989.