Effects of soil moisture, phosphorus and zinc on isoenzymes activity and banding patterns of peroxidase in potato plant

Paper Details

Research Paper 01/01/2016
Views (374) Download (11)
current_issue_feature_image
publication_file

Effects of soil moisture, phosphorus and zinc on isoenzymes activity and banding patterns of peroxidase in potato plant

Mahdi Mirashzadeh, Mostafa Valizadeh, Rahim Motalebifard, Majid Noruzi, Mahdi Taghizadegan, Mozhgan Shirinpour, ShahinUstan
J. Bio. Env. Sci.8( 1), 34-42, January 2016.
Certificate: JBES 2016 [Generate Certificate]

Abstract

Water deficit stress is a major abiotic factor that limits crop production. Hence plant Nutrition can have play-determining role in moderating the adverse effects of water deficit stress. This research was conducted as a factorial experiment based on randomized complete blocks design with zinc (Zn) at three levels (0, 10 and 20 mg Zn per kg dries soil as ZnSO4•7H2O), phosphorus (P) at three levels (0, 30 and 60 mg P per kg dry soil as Calcium (Ca) (H2PO4)2•H2O) and soil moisture at three levels (0.5FC-0.6FC, 0.7FC-0.8FC and 0.9FC-FC) using three replications under greenhouse conditions. The results showed that the moderate (0.7FC-0.8FC) and severe water deficit conditions (0.5FC-0.6FC) decreased significantly activity of peroxidase isozymes (POX) than to the enzyme activity in full irrigated (0.9FC-FC) conditions (P< 0.01).The higher activity of peroxidase isozymes appeared in POX1 under the moderate water deficit condition and the lowest related to POX5 isozyme under severe water deficit condition. In addition, the main effect of Zn and two way interaction of Zn × soil moisture were significant on the enzymatic activity of POX2, POX3 and POX4 isozymes. The highest activity of peroxidase isozymes resulted for POX2 at application of10 mg Zn per kg of soil. The two ways interaction of soil moisture × Zn for POX3, POX2 and POX4 showed that the effect of Zn application on these esozymes were significant only under severe water deficit condition the highest activity of POX2 and POX3 were obtained at application of 10 mg Zn per kg dried soil and for POX4 under using of 20 mg Zn per kg soil condition.

VIEWS 11

Ahn M, Schofield G. 2005. Effects of phosphorus fertilizer supplementation on antioxidant enzyme activities in tomato fruits. Journal of Agricultural and Food Chemistry 53(5), 1539-45.

Cakmak I. 2000. Possible role of zinc in protecting plant cells from damage by reactive oxygen species. The New Phytologist 116, 185-205.

Coleman JE. 1992. Zinc proteins: enzymes, storage proteins, transcription factors and replication proteins. Annual Review of Biochemistry 61, 897-946.

Cruz De Carvalho MH. 2008. Drought stress and reactive oxygen species. Plant Signaling and Behavior 3, 156-165.

Gaber MA. 2010. Antioxidative defense under salt stress. Plant Signaling & Behavior 5, 369-374.

Garg K, Burman U, Shyam K. 2004. The influence of phosphorus nutrition on the physiological response of moth beans genotypes to drought. Journal of Plant Nutrition and Soil Science 167, 503-508.

Gupta KJ, Stoimenova M, Kaiser WM. 2005. In higher plants, only root mitochondria, but not leaf mitochondria reduce nitrite to NO, in vitro and in situ. Journal of Experimental Botany 56, 2601–2609.

Han SF, Deng R, Cao Y, Wang Y, Xiao K. 2007. Photosynthesis and active-oxygen-scavenging enzyme activities in rice varieties with different phosphorus efficiency under phosphorus stress. Ying Yong Sheng Tai Xue Bao 18(11), 2462-2462.

Jin J, Wang G, Liu X, Pan SJ. 2006. Interaction between phosphorus nutrition and drought on grain yield. Journal of Plant Nutrition 29, 1433-1449.

Lutfor Rahman SM, Mackay A, Mesbah Uddin ASM. 2002. Superoxide Dismutase Activity, Leaf Water Potential, Relative Water Content, Growth and Yield of a Drought-Tolerant and a Drought-Sensitive Tomato (Lycopersicon esculentum Mill.) Cultivars Plant Science 54, 16-22.

Morsy AA, Salama KH, Kamel HA, Mansour MM. a2012. Effectes of heave metals on plasma membrane lipids and antioxidant enzymes of Zygophyllum species. Eurasia of Biosciences 6, 1-10.

Motalebifard R, Najafi N, Oustan S, Nyshabouri MR, Valizadeh M. 2013. The combined effects of phosphorus and zinc on evapotranspiration, leaf water potential, water use efficiency and tuber attributes of potato under water deficit conditions. Scientia Horticulturae 162, 31-38.

Olson PD, Varner JE. 1993. Hydrogen peroxidase and lignification. Journal of Plant 4, 887-892.

Raghothama   KG.   1999.   Phosphate   acquisition. Plant Molecular Biology 50, 665-693.

Reddy AR, Chaitanya KV, Vivekanandan M. 2004. Drought induced responses of photosynthesis and antioxidant metabolism in higher plants. Journal of Plant Physiology 161, 1189–1202.

Rodriguez D, Goudriaan J. 1995. Effects of phosphorus and drought stress on dry-mater and phosphorus allocation in wheat. Journal of Plant Nutrition 18, 2501-2517.

Sun C, Du W, Chang X, Xu X, Zhang Y, Sun D, Shi J. 2010. The effects of drought stress on the activity of phosphatase and its protective enzymes in pigweed leaves. African Journal of biotechnology 9, 825-833.

Terzi R, Krdiogl A. 2006. Droughts stress tolerasce and the antioxidant enzyme system in Ctenanthe Setosa. Acta Biologica Botanica 48, 89-96.

Valizadeh M, Mohayeji Mz, Yasinzadeh N, Nasrullahzadeh S, Moghaddam M. 2011. Genetic diversity of synthetic alfalfa generates and cultivars using tetrasomic inherited allozyme markers. Journal of Agricultural Science & Technology 13, 425-430.

Vallee BL, Falchuk KH. 1993. The biochemical basis of zinc physiology. Physiological reviews 73, 79-118.

Waraich EA, Amad R, Ashraf MY, Ahmad M. 2011. Improving agricultural water uses efficiency by nutrient management. Journal of plant physiology 61(4), 291-304

XiYao W, Zou X, Wang J. 2009. Drought tolerance enhanced by phosphorus deficiency in potato plants. Zhongguo zuo wu xue hui 35, 875-883.

Yong TL, Zongsuo S, Hongbo D. 2006. Effect of water deficits on the activity of anti-oxidative enzymes and osmoregulation among three different genotypes of radix astragali at seeding stages colloids and surfaces. Biointerfaces 49, 60–65.

Yuncai H, Schmidhalter U. 2006. Drought and salinity: A comparison of their effects on mineral nutrition of plants. Journal of Plant Nutrition and Soil Science 168, 541-549.

Zhang F, Guo Jk, Yang YL, He WL, Zhang LX. 2004. Change in the pattern of antioxidant enzymes in wheat exposed to water deficit and rewatering. Acta Physiologiae Plantarum Plant 26, 345-352.