Effects of water stress on: mycorrhizal root colonization, chlorophyll pigment, number of spore, mycorrhizal dependency and nodules of two mycorrhizal leguminous plants (Tephrosia vogelii and Vigna subterranea) at an early stage of growth

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Effects of water stress on: mycorrhizal root colonization, chlorophyll pigment, number of spore, mycorrhizal dependency and nodules of two mycorrhizal leguminous plants (Tephrosia vogelii and Vigna subterranea) at an early stage of growth

Tsoata Esaïe, Njock Siméon Raphaël, Youmbi Emmanuel, Nwaga Dieudonné
J. Bio. Env. Sci.7( 5), 96-108, November 2015.
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The present study carried out in a randomized blocks design, aimed at evaluating the effects of water stress on root colonization, mycorrhizal dependency, number of spores and number of root nodules of mycorrhizal T. vogelii and V. subterranea. The four treatments applied were: 90, 60, 30 and 15 for control, low, average, severe stress respectively, with or without AMF and for 31days. Results obtained showed that, with respect to stress severity, the harmful effects of water stress were more marked with T. vogelii compaired to V. subterranea. However, the mycorhization alleviated the negative impacts of the water stress thus favored root nodule installation. The AMF increased significantly the number of root nodules for the two leguminous plants used in this study for both unmycorrhizal or mycorrhizal plants. One could thus conclude that, the mycorrhizal inoculum used could serve as an effective microbial material for alleviating the harmful impacts of water stress on leguminous plants growth at an early stage of development.


Abdelmoneim TS, Tarek Moussa AA, Almaghrabi OA, Hassan Alzahrani S, Abdelbagi I. 2014. Increasing plant tolerance to drought stress by inoculation with arbuscular mycorrhizal fungi. Life Science Journal 11(1), 10-17.

Albert RS, Thornber JP. 1977. Water stress effects on the content and organization of chlorophyll in mesophyll and bundle sheath chloroplast. Plant Physiology 59, 351-353.

Allahverdiyev T. 2015. Effect of drought stress on some physiological traits of durum (Triticum durum Desf.) and bread (Triticum aestivum L.) wheat genotypes. Journal of Stress Physiology & Biochemistry 11(1), 29-38.

Ameyaw GCE, Doku EV. 1983. Effects of soil moisture stress on the reproductive efficiency and yield of the bambara groundnut (Voandzeia subterranea). Crop Science 28, 23-29.

Anchirina VM, Viridoe EK, Bennett-Lartey SO. 2001. Enhancing sustainable production and genetic resource conservation of bambara groundnut. A survey of indigenous agricultural knowledge systems. Outlook on Agriculture 30(4), 281-288.

Anjum F, Yaseen M, Rasul E, Wahid A, Anjum S. 2003. Water stress in barley (Hordeum vulgare L.) I. Effect on chemical composition and chlorophyll contents, Pakistan Journal of Agriculture Science 40, 45-49.

Augé RM.  2001.  Water  relations,  drought  and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza 11, 3-42.

Aune D, De Stefani E, Ronco A, Boffetta P, Deneo Pellgrini H, Acosta G, Mendilaharsu M. 2009. Legume intake and the risk of cancer: a multisite case-control study in Uruguay. Cancer Causes & Control 20, 1605-1615.

Baligar VC, Fageria NK. 2007. Agronomy and physiology of tropical cover crops. Journal of Plant Nutrition 30, 1287-1339.

Belmain SR, Zamoah BA, Nyirenda SP, Kamanula JF, Stevenson PC. 2012. Highly variable insect control efficacy of Tephrosia vogelii chemotypes. Journal of Agriculture and food Chemistry 60, 10055-10063.

Buchanan BB, Gruissem W, Jones RL. 2000. Biochemistry & molecular Biology of Plants. American Society of Plant Physiol. Rockville, MD, p. 49-72.

Chaussod R, Nouaim R. 1996. Rôles des mycorhizes dans l’alimentation hydrique et minérale des plantes, notamment des ligneux de zones arides. Cahiers options méditerranéennes 20, 9-26.

Chen X, Song F, Liu F, Tian C, Liu S, Xu H, Zhu X. 2014. Effect of different Arbuscular Mycorrhizal Fungi on Growth and Physiology of Maize at Ambient and low Temperature Regimes. The scientific World Journal 2014, Article ID 956141, 7 p.

De La Fuente M, Lopez-Pedrouso M, Alonso J, Santalla M, De Ron AM, Alvarez G, Zapata C. 2012. In-Depth characterization of the phaseolin protein diversity of common bean [Phaseolus vulgaris L.] based on two-dimensional electrophoresis and mass spectrometry. Food Technology & Biotechnology 50(3), 315-325.

Finlay RD. 2008. Ecological aspects of mycorrhizal symbiosis: with special emphasis on the functional diversity of interactions involving the extra radical mycelium. Journal of Experimental Botany 59, 115 – 1126.

Fivawo N, Msolla S. 2011. The diversity of common bean Landraces in Tanzania. Tanzania Journal of Natural and Applied Sciences 2(1), 337-351.

Gadzirayi CT, Mutandwa E, Mivale M, Chindundu M. 2009. Utilization of Tephrosia vogelii in controlling ticks in dairy cow by small scale commercial farmers in Zimbabwe. African Journal of Biotechnology 9(17), 4134-4136.

Gerdemann JW, Nicolson TH. 1963. Spores of mycorrhizal endogone species extracted from soil by wet sieving and decanting. Mycology Society 46, 235-244.

Gholamhoseini M, Ghalavand A, Dolatabadian A, Jamshidi E, Khodaei-Jorghan A. 2013. Effects of arbuscular mycorrhizal inoculation on growth, yield, nutrient uptake and irrigation water productivity of sunflowers grown under drought stress. Agricultural Water Management 117, 106-114.

Gianinazzi S, Gollotte, Binet MN, Van Tuinen D, Redecke D and Wipf D. 2010. Agroecology: the key role of arbuscular mycorrhizas in ecosystem services. Mycorrhiza 20(8), 519-530.

Goicoechea N, Merino S, Sanchez Diaz. 2004. Contribution of mycorrhizal fungi (AMF) to the adaptations exhibited by the deciduous shrub Anthyllis cytisoides under water deficit. Physiologia Plantarum 122, 453-464.

Guettouche R. 1990. Contribution à l’indentification des caractères morphologiques d’adaptation à la sécheresse chez le blé dur (Triticum durum Desff). Thèse diplôme d’agronomie approfondie, mise en valeur du milieu.INA El Harrach, 115 p.

Irigoyen JJ, Emerich DW, Sanchez-Diaz M. 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum 84, 55-60.

Jacobson KM. 1997. Moisture and substrate stability determine VA-mycorrhizal fungal community distribution and structure in an arid grassland. Journal Arid Environment 35, 59-75.

Jain M, Mittal M, Gadre R. 2013. Effect of PEG-6000 imposed water deficit on chlorophyll metabolism in maize leaves. Journal of stress Physiology & biochemistry 9(3), 262-271.

Jeyaramraja PR, Meenakshi SN, Kumar RS, Joshi SD, Ramasubramanian B. 2005. Water deficit induced oxydative damage in tea (Camellia sinensi) plant. Journal Plant Physiology 162, 413-419.

Kalume MK, Losson B, Angenot L, Tits M, Wauters JN, Fèdèrich M, Saegerman C. 2012. Rotenoïd content and in vitro acaricidal activity of Tephrosia vogelii leaf extract on the tick Rhipicephalus appendiculatus. Verterinary Parasitology 190(1), 204-209.

Kim YC, Glick BR, Bashan Y, Ryu CM. 2012. Enhancement of plant drought tolerance by microbes. In: Aroca R (ed) plant responses to drought stress: from morphological to molecular features. Springer Verlag, Berlin & Heidelberg, Germany, 343-413.

Kormanik PP, Mcgraw AC. 1982. Quantification of vesicular–arbuscular mycorrhizal in plants roots. In: Methods and principles of mycorrhizal research. Schenck NC (ed). APS St. Paul, Minnesota, USA 45p.

Lacroix B, Assoumou Ndong Y, Sangwan RS. 2003. Efficient in vitro direct shoot regeneration system in bambara groundnut (Vigna subterranea (L.) Verdc.). Plant Cell Reports 21, 1153-1158.

Litchtenthaler HK, Buschmann C. 2001; Chlorophylls and Carotenoids: Measurement and characterization by UV-VIS spectroscopy. Current Protocols in Food Journal Chemistry 2, 1-8.

Magreta R, Chirwa R, Nyrirenda S, Tenywa JS, Taulya G, Kawube G, Santos L. 2011. Optimised pest management with Tephrosia on legume cropping systems in Malawi: a baseline study. In: 10th African Crop Science Conference Proceedings, Maputo, Mozambique 10-13 October 2011. African Crop Sciences Society 199-204 p.

Mahajan S, Tuteja N. 2005. Cold, salinity and drought stresses: an overview. Archives Biochemistry and Biophysics 444, 139-158.

Miransari M. 2010. Contribution of arbuscular mycorrhizal symbiosis to plant growth under different types of soil stresses. Review article. Plant Biology 12, 563-569.

Mohamed HA, Shukry AO, Sokol K. 2000. The impact of pesticides on arbuscular mycorrhizal and nitrogen-fixing symbioses in legumes. Applied Soil Ecology 14, 191-200.

Monakhova OF, Chernyadev IJ. 2002. Protective role of kartolin-4 in Wheat plants exposed to soil drought. Applied Biochemistry Microbiology 38, 373-380.

Mshana NR, Abbiw DK, Addae-Mensah F, Adjanouhoun E, Ahyi MRA, Ekpera JA, Enow-Drock EG, Noamesi MA, Odunlami H, Oteng-Yeboah AA, Sarpong K, Sofowora A, Tackle AN. 2000. Traditional medicine and pharmacopeia: contributing to revision of ethnobotanical and floristic studies in Ghana. OAU/Scientific, Technical and Research commission, 383p.

Nkenda PA, Mtei K, Ndakidemi PA. 2014. Pesticidal efficacy of Tephrosia vogelii and Tithonia diversifolia against field insect pests of common bean [Phaseolus vulgaris L.] within African farming communities. African Journal of Applied Agricultural Sciences and Technologies 2(1), 9-26.

Nayya H, Gupta D. 2006. Differential sensitivity of C3 and C4 plants to water deficit stress: association with oxidative stress and antioxidants. Environmental and Experimental Botany 58, 106-113.

Obomanu FG, Ogbalu OK, Gabriel UU, Fekarurhobo GK, Abadi SU. 2007. Piscicidal effects of Lepidagathis alopecuriodes on mudskipper, Periophthalmus papillis from the Niger Delta. Research Journal of Applied Sciences 2(4), 282-387.

Osonubi O, Bakare ON, Mulongo K. 1992. Interactions between drought stress and vesicular-mycorrhiza on the growth of Faidhera albida (syn. Acacia albida) and Acacia nilotica in sterile and non-sterile soils. Biology and Fertility of Soils 14, 159-165.

Owonubi SJ, Ojuederie OB, Olayode MN. 2011. Organogenesis of Bambara groundnut (Vigna subterranean (L.) Verdc.) through in vitro culture from nodal segments. International Journal of Plant Developmental Biology 5(1), 33-57.

Parniske M. 2008. Arbuscular mycorrhiza: the mother of plant root endosymbiosises. Nature Review of Microbiology 6 (10), 763-775.

Plenchette C, Fortin JA, Furlan V. 1983. Growth responses of several plant species to mycorrhizae in a soil of moderate P-fertility. I. Mycorrhizal dependency under field conditions. Plant and Soil 70, 199-209.

Richardson E, Diseker EG. 1965. Establishing and maintaining roadside cover in the piedmont plateau of Georgia. Agronomy Journal 57, 561-564.

Ruiz-Lozano JM, Aroca R. 2010. Modulation of aquaporin genes by the arbuscular mycorrhizal symbiosis in relation to osmotic stress tolerance. In: Seckbach J, Grube M (eds) Symbioses and stress: joint ventures in biology, cellular origin, life in extreme habitats and astrobiology. Springer Science Business Media, Dordrecht, p.359-374.

Shaul O. 2002. Magnesium transport and function in plants: the tip of the iceberg. Biometals 15, 309-323.

Smith SE, Read DJ. 2008. Mycorrhizal symbiosis, Academic, London, UK, 3rd edition 31-67 p.

Sochacki P, Ward JR, Mitchell Cruzan B. 2013. Consequenes of mycorrhizal colonization for Pisiqueta morphotypes under drought stress. International Journal of Plant Sciences 174(1), 65-73.

Solomon SG, Cheikyula JO, Anju DT. 2014. Behavioral responses of Heterobranchus longifilis juveniles. Val (Pisces: 1840) exposed to freeze-dried Bark extract of Tephrosia vogelii as an anesthetic. Journal of Stress Physiology & Biochemistry 10(1), 82-92.

Subramanian KS, Santhanakrishnan P, Balasubramanian P. 2006. Response of field grown tomato plants to arbuscular mycorrhizal fungal colonization under varying intensities of drought stress. Scientia Horticulturae 107, 245-253.

Tahri El, Houssine, Belabed Abdelmajid, Sadki Khadija. 1998. Effet d’un stress osmotique sur l’accumulation de proline, de chlorophylle et des ARNm codant pour la glutamine synthétase chez trois variétés de blé dur (Triticum durum). Bulletin de l’Institut Scientifique 21, 81-87.

Tardieu F. 2005. Plant tolerance to water deficit: Physical limits and possibilities for progress. Geophysical Sciences 337, 57-67.

Tomati U, Veri G, Gall E. 1978. Effect of water stress on photosynthesis and nitrate reductase activity in maize plants. Review in Agronomy 12, 119-122.

Tsoata E, Njock SR, Youmbi E, Nwaga D. 2015. Early effects of water stress on some biochemical and mineral parameters of mycorrhizal Vigna subterranea (L.) Verdc. (Fabaceae) cultivated in Cameroon. International Journal of Agronomy and Agriculture Research (IJAAR) 7(2), 21-35.

Westphal E, Ferwerda JD, Van Gils-Meeus HAE, Mustsaers HJW, Westphal Stevels JMC. 1985. Cultures vivrières tropicales avec reference special au Cameroun. Pudoc. Wageningen, Netherlands 514 p.

Wu C, Wang Q, Xie B, Wang Z, Cui J, Hu T. 2011. Effects of drought and salt stress on seed germination of three leguminous species. African Journal of Biotechnology 10(78), 17954-17961.

Wu QS, Xia RX. 2006. Arbuscular mycorrhizal fungi influence growth, osmotic adjustement and photosynthesis of citrus under well-watered and water stress conditions. Journal of Plant Physiology 163, 417-425.

Yadav SK, Jyothi Lakshmi N, Vikram Singh, Amol Patil, Yogesh Kumar Tiwari E, Nagendram, Sathish P, Vanaja M, Maheswari M, Venkateswarlu B. 2013. In vitro screening of Vigna mungo genotype for PEG induced moisture deficit stress. Indian Journal of Plant Physiology 18(1), 55-60.

Zayed MA, Zeid IM. 1998. Effect of water and salt stresses on growth, chlorophyll, mineral ion and organic solute contents and enzymes activity in mung bean seedlings. Biologia Plantarum 40(3), 351-356.

Zhang M, Duan L, Zhai Z, Li J, Tian X, Wang B, HE Z, Li Z. 2004. Effects of plants growth regulators on water deficit-induced yield loss in soybean. Proceedings of the 4th International Crop Science A Congress, Brisbane, Australia, 49-76 p.

Ziska LH. 2011. Climate change, carbon dioxide and global crop production: food security and uncertainty. In: Diner A, Mendelsohn R (eds) handbook on climate change and agriculture. Edward Elgar Publishing Ltd, Cheltenham and Camberley, UK, 9-31 p.