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Efficacy of bio control agents for management of Phytophthora megasperma causes of collar rot of peas

Research Paper | April 1, 2019

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Zafar Iqbal, Muhammad Usman Ghazanfar, Waqas Raza, Salman Ahmad, Muhammad Zohaib Anjum

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Int. J. Biosci.14( 4), 281-285, April 2019

DOI: http://dx.doi.org/10.12692/ijb/14.4.281-285


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Pea (Pisum sativum L.) is a popular pulse crop in the world. It is attacked by various biotic diseases but collar rot caused by Phytophthora megasperma occurs in severe form and causes colossal losses every year. At present this disease is controlled by application of fungicides. But due to serious health hazards of pesticides and environmental concerns, non-chemical approaches are preferred these days. Bio control agents are considered safe and effective to manage soil borne plant diseases. In current work, three characterized Trichoderma isolates viz. Trichoderma harzianum, T. viride and T. asperellum and one isolate of Bacillus subtilis were testedagainst the pathogen P. megasperma by using different antagonistic assays i.e. dual culture, volatile metabolites and nonvolatile metabolites. All the treatments effectively reduced the radial growth of the pathogen during dual culture assay. Nonvolatile metabolites displayed higher growth inhibition of the pathogen as compared to volatile metabolites. T. asperellum showed maximum inhibition of the pathogen (47.60%) while minimum inhibition was shown by T. viride (26.90%). Present studies conclude that biocontrol agents can be successfully used to manage soil borne plant diseases with no adverse effects or health hazards. These studies will pave the way for effective eco-friendly management of plant diseases in future.


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Efficacy of bio control agents for management of Phytophthora megasperma causes of collar rot of peas

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