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Efficacy of different Plant essential oils against Penicillium expansum causing fruit rot of grapes

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Research Paper 01/02/2020
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Efficacy of different Plant essential oils against Penicillium expansum causing fruit rot of grapes

Muhammad Zunair Karamat, Gulshan Irshad, Abid Riaz, Salman Ghuffar, Farah Naz, Hafiz Muhammad Ashfaq, Muhammad Usman Raja, Abdul Qadir, Khalid Mehmood, Hanli Yang, Junjie Guo
Int. J. Biosci.16( 2), 516-522, February 2020.
Certificate: IJB 2020 [Generate Certificate]
Key: Fruit rotGrapesmanagementPenicillium expansumPlant essential oil

Abstract

Penicillium rot (PR) caused by Penicillium expansum is a severe fruit rot of grapes in Pakistan, causing serious threat for the environment and responsible to market losses. The objective of this study is to find out some effective plant essential oils as an alternative to synthetic fungicides against P. expansum causing fruit rot of grapes. For this purpose, three selected plant essential oils (EOs) viz. Olive (EO), Citrus peel (EO) and Fenugreek (EO) at 400, 600 and 800 ppm concentrations were studied by using different methods under in vitro condition as well as application on grapes against previously isolated culture of P. expansum designated as (Isolate ID. APE10TL3) on grapes bunches respectively. Results showed that Citrus peel (EO) at all concentrations showed significant result to control the mycelial growth (89.3, 94 and 97.5%) in contact assay method as well as 90.1, 96.3 and 99% growth inhibition regarding fungal culture transfer (FCT) experiment while, in case of well diffusion method 41%, 46% and 52% growth inhibition was recorded at 7 day of incubation followed by olive (EO) and fenugreek (EO) as compared to control 0% growth inhibition was measured. Moreover, the Citrus peel (EO) was further evaluated for the presence of anti-fungal compounds viz. terpene, alkaloids, phenolic and saponins employing standard protocols and found positive for the presence of all compounds. During the application of citrus peel essential oil at (800 ppm) concentration on fruit bunches for the determination of decaying percentage. The result showed 12.53% decay caused by P. expansum on treated bunches up to six days of storage and control was 85.14% calculated. Keeping in all view, Citrus peel (EO) possessing good inhibitory action upon Penicillium expensum might be used as a potential candidate for preservation and extension of shelf-life of grapes commercially.

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Ali K, Maltese F, Choi YH, Verpoorte R. 2010. Metabolic constituents of grapevine and grape-derived products. Phytochemistry Reviews 9(3), 357-378.

Anwar F, Rashid U. 2007. Physico-chemical characteristics of Moringa Oleifera seeds and seed oil from a wild provenance of Pakistan. Pakistan Journal of Botany 39(5), 1443-1453.

Bagamboula CF, Uyttendaele M, Debevere J. 2004. Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food microbiology 21(1), 33-42.

Dauria F, Tecca M, Strippoli V, Salvatore G, Battinelli L, Mazzanti G. 2005. Antifungal activity of Lavandula angustifolia essential oil against Candida albicans yeast and mycelial form. Medical mycology Journal 43(5), 391-396.

Dayan FE, Cantrell CL, Duke SO. 2009. Natural products in crop protection. Bioorganic & medicinal chemistry 17(12), 4022-4034.

Duduk N, Lazarevic M, Zebeljan A, Vasic M, Vico I. 2017. Blue mould decay of stored onion bulbs caused by Penicillium polonicum, P. áglabrum and P. áexpansum. Journal of Phytopathology 165(10), 662-669.

Evans WC. 1997. Trease and Evans phamacognosy. (14th ED) Harcourt Brace and Company. Asia Pvt. Ltd. Singapore 343.

Feng W, Zheng X. 2007. Essential oils to control Alternaria alternata in vitro and in vivo. Food control 18(9), 1126-1130.

Ghaouth AE, Arul J, Grenier J, Asselin A. 1992. Antifungal activity of chitosan on two post harvest pathogens of strawberry fruits. Phytopathology 82, 398-402.

Ghuffar S, Irshad G, Aslam MF, Naz F, Mehmood N, Hamzah AM, Mehmood A, Zeshan MA, Rehman S, Gleason ML. 2018. First report of Mucor fragilis causing bunch rot of grapes in Punjab, Pakistan Plant Disease 102(09), 1858.

Ghuffar S, Irshad G, Naz F, Rosli HB, Hyder S, Mehmood N, Zeshan MA, Raza MM, Mayer CG, Gleason ML. 2018. First report of two Penicillium spp. causing post-harvest fruit rot of grapes in Pakistan Plant Disease 102(5), 1037.

Ghuffar S, Irshad G, Shahid M, Naz F, Riaz A, Khan MA, Mehmood N, Sattar A, Asadullah HM, Gleason ML. 2018. First report of Alternaria alternata causing fruit rot of grapes in Pakistan Plant Disease 102(08), 1659.

Isman MB. 2000. Plant essential oils for pest and disease management. Crop protection 19(8-10), 603-608.

Jamil A, Shahid M, Khan MM, Ashraf M. 2007. Screening of some medicinal plants for isolation of antifungal proteins and peptides. Pakistan journal of Botany 39(1), 211-221.

Javed S, Javaid A, Anwar W, Majeed R, Akhtar R, Naqvi S. 2017. First report of Botrytis bunch rot of grapes caused by Botrytis cinerea in Pakistan. Plant Disease 101(6), 1036.

Kokate CK. 1999. Practical Pharmacognasy. (4th ED) Vallabh Parkashan Publications, New Dehli, India 115.

Mari M, Leoni O, Iori R, Cembali T. 2002. Antifungal vapour‐phase activity of allyl‐ isothiocyanate against Penicillium expansum on pears. Plant pathology 51(2), 231-236.

Mohamedy ERS, Gamal ENG, Bakeer ART. 2015. Application of chitosan and essential oils as alternatives fungicides to control green and blue moulds of citrus fruits. International Journal of Current Microbiology and Applied Science 4(6), 629-643.

Naz F, Qamarunnisa S, Shinwari ZK, Azhar A, Ali SI. 2013. photochemical investigations of Tamarix indica and Tamarix passernioides Del.ex Desv. Leaves from Pakistan. Pakistan journal of Bot\any 45(5), 1503-1507.

Neri F, Donati I, Veronesi F, Mazzoni D, Mari M. 2010. Evaluation of Penicillium expansum isolates for aggressiveness, growth and patulin accumulation in usual and less common fruit hosts. International journal of food microbiology 143(3), 109-117.

Nunes CA. 2012. Biological control of postharvest diseases of fruit. European Journal of Plant Pathology 133(1), 181-196.

Patel RM, Jasrai YT. 2011. Evaluation of Fungitoxic Potency of Medicinal Plants Volatile Oils (VOs) against Plant Pathogenic Fungi. Pesticide Research Journal 23(2), 168-171.

Prusky D. 2011. Reduction of the incidence of postharvest quality losses, and future prospects. Food Security 3(4), 463-474.

Sahin F, Karaman I, Gulluce M, Ogutcu H, Sengul M, Adıguzel A, Kotan R. 2003. Evaluation of antimicrobial activities of Satureja hortensis L. Journal of Ethnopharmacology 87(1), 61-65.

Satish S, Mohana DC, Raghavendra MP, Raveesha KA. 2007. A2ntifungal activity of some plant extracts against important seed borne pathogens of Aspergillus sp. The Journal of Technology 3, 109-119.

Seema MSS, Sreenivas ND, Devaki NS. 2011. In vitro studies of some plant extracts against Rhizoctonia solani Kuhn infecting FCV tobacco in Karnataka Light Soil, Karnataka, India. Journal of Agriculture science and Technology 7(5), 1321-1329.

Tzortzakis NG, Economakis CD. 2007. Antifungal activity of lemongrass (Cympopogon citratus L.) essential oil against key postharvest pathogens. Innovative Food Science & Emerging Technologies 8(2), 253-258.

Velázquez NMJ, Avila SR, Palou E, Lopez MA. 2013. Antifungal activity of orange (Citrus sinensis var. Valencia) peel essential oil applied by direct addition or vapor contact. Food Control 31(1), 1-4.

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