Efficacy of entomopathogenic nematodes Steinernema kraussei to control the fruit fly larvae
Paper Details
Efficacy of entomopathogenic nematodes Steinernema kraussei to control the fruit fly larvae
Abstract
Entomopathogenic nematodes (EPN’s) belongs to family Steinernematidae and Heterorhabditidae which are obligate parasites of various species of insect pests and used as a bio-control agent. Nematode juvenile enter through the natural opening of insect larvae’s and release the bacteria into the body of insect which release the toxic substances into the insect body and kill the insect larvae’s in 24-72 hours depends on the species of insect. The objective of current research was to check out the reproductive potential of EPN’s (Steinernema kraussei) on fruit fly larvae’s at different levels. The reproductive potential was recorded at different days (1, 2, 3, 4 and 5), at different concentrations (100IJs, 200IJs, 300IJs and 500IJs) and different storage time (2, 4, 6 and 8 weeks) of culture. The maximum progeny was recorded 29367 at day 5 and at 500IJs concentration the maximum reproductive potential was recorded 30733 and the maximum population 40933 of nematode was recorded at 2 week old culture. So the results are clearly indicated that reproductive potential of entomopathogenic nematodes (Steinernema kraussei) was increased day by day, with the increased in concentration of nematode the population of nematodes (EPN’s) also increased but with the increased in storage time of EPN’s cultures the reproductive potential was decreased.
Armes NJ, Bond GS, Cooters RJ. 1992. The laboratory culture and development of Helicoverpa armigera. Natural Resources Institute Bulletin (Chatham, UK: Natural Resources Institute) pp 57.
Brown IM, Gaugler R. 1997. Temperature and Humidity Influence Emergence and Survival of Entomopathogenic Nematodes 43, 363-375.
De Groote H. 2002. Maize yield losses from stem borers in Kenya. International Journal Tropical Insect Science 22, 89-96.
Dunphy GB, Webster JM. 1986. Temperature Effects on the Growth and Virulence of Steinernema feltiae Strains and Heterorhabditis heliothidis. Journal of Nematology 18, 270-2.
Dutky SR, Thompson JV, Cantwell GE. 1964. A technique for the mass propagation of the DD-136 nematode. Journal Insect Pathology 6, 417-422.
Ehlers RU. 1996. Current and future use of nematodes in bio control. Practice and commercial aspects in regard to regulatory policies. Biology Control Science Technology 6, 303-316.
Ehlers RU. 2001. Mass production of entomopathogenic nematodes for plant protection. Applied Microbiology and Biotechnology 56, 623-633.
Glaser RW. 1932. Studies on Neoaplectana glaseri, a nematode parasite of the Japanese beetle (Popillia japonica). New Jersey Department of Agriculture Circular 211: 3-34.
Grewal PS, Lewis EE, Gaugler R, Campbell JF. 1994. Host ending behavior as a predictor of foraging strategy of entomopathogenic nematodes. Parasitology 108, 207-215.
Hominick WM. 2002. Biogeography: Entomopathogenic nematology. CABI Publishing, Oxon, New York pp. 115.
Kruitbos LM, Heritage S, Hapca S, Wilson MJ. 2010. The influence of habitat quality on the foraging strategies of the entomopathogenic nematodes Steinernema carpocapsae and Heterorhabditis megidis. Parasitology 137, 303-309.
Nguyen KB, Smart GC. 1992. Life cycle of Steinernema scapterisci Nguyen and Smart 1990. Journal of Nematology 24, 160-169.
Poinar GO. 1976. Description and biology of a new insect parasitic rhabditoid, Heterorhabditis bacteriophora n. gen., n. sp. (Rhabditida: Heterorhabditidae n. fam.) Nematology 21, 463-470.
Resh V, Ring H, Carde T. 2009. Encyclopedia of Insects (2ed.). USA Academic Press ISBN. 0-12-374144-0.
Steel RDD, Torrie JH, Dicky D. 1997. Principles and Procedures of Statistics: A Biometrical Approach. 3rd Ed. Mc. Graw Hill Book Co. Inc. New York, USA.
Steiner G, 1929. Neoaplectana glaseeri n. g. sp. (Oxyuridae) a new endemic parasite of the Japsnese beetle (Popillia japonica). Journal of the Washington Academy of Sciences 19, 436-440.
White GF. 1927. A method for obtaining infective nematode larvae from culture. Science 66, 302-303.
Willmott DM, Hart AJ, Long SJ, Edmondson RN, Richardson PN. 2002. Use of a cold-active entomopathogenic nematode Steinernema kraussei to control overwintering larvae of the black vine weevil Otiorhynchus sulcatus (Coleoptera: Curculionidae) in outdoor strawberry plants. Nematology 4, 925-932.
Owais Yasin, Sumaira Saeed, Nazir Javed, Imran Ul Haq, Qaiser Shakeel, Yasir Ali, Hafiz Muhammad Usman Aslam, Qurban Ali, Muhammad Asif, Khizar Razzaq, 2018. Efficacy of entomopathogenic nematodes Steinernema kraussei to control the fruit fly larvae. Int. J. Biosci., 13(1), 470-476.
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