Paper Details

Efficiacy of SCoT and ISSR marekers in assesment of tomato (Lycopersicum esculentum Mill.) genetic diversity.

Ahmad Shahlaei, Sepideh Torabi, Mahmood Khosroshahli

DOI: http://dx.doi.org/10.12692/ijb/5.2.14-22

Int. J. Biosci. 5(2), 14-22. July, 2014. (PDF)

Abstract:

Two molecular marker systems, SCoT and ISSR were used for genetic diversity analysis of 10 Tomato (Lycopersicum esculentum L.) accessesions. Using 10 selected SCoT primers 83 bands were generated, of which 30 (36.14%) were polymorphic. 10 selected ISSR primers amplified 86 bands with 20 (23.25%) being polymorphic. Average PIC values for SCoT and ISSR marekers were 0.142 and 0.088 respectively. Mean RP values for SCoT and ISSR marekers were 1.88 and 1.55 respectively. The 10 accessesion were clustered into 3 major groups based on the SCoT analysis and 2 major groups based on the ISSR analysis with UPGMA. The PCA Analysis confirmed the results of the clustering. The results also demonstrate that the SCoT marker system is useful for identification and genetic diversity analysis of tomato accessesions. In general, SCoT molecular marker was informative than ISSR molecular system.