Paper Details

Efficiency of different protocols for RNA extraction from bean (Phaseolus vulgaris L.) leaves

Maryam Boroujerdnia, Mohammad Reza Bihamta, Khalil Alami Said, Vahid Abdossi

DOI: https://dx.doi.org/10.12692/ijb/5.12.390-398

Int. J. Biosci. 5(12), 390-398. December, 2014. (PDF)

Abstract:

Purification of intact ribonucleic acids is fundamental for the study of gene expression.  It has been difficult to extract a good quality and quantity of total RNA from plants with high levels of protein,  phenols and polysaccharides. Therefore, Six RNA extraction method was tested to determine which of them is more efficient on bean leaves. It was observed that the CTAB-active charcoal and CTAB-SDS methods presented more pure RNA, with no degradation, suitable for further gene expression analysis. Also, quality of isolated RNA from both was further checked by cDNA synthesis. Extracted RNA was found suitable for further molecular applications such as reverse transcription and cDNA library construction.