Int. J. Biosci.14( 6), 110-121, June 2019
The information about sulphur oxidising bacteria from rock phosphate mine area Hazara Pakistan is still unknown. This study was conducted to isolate sulphur oxidising bacteria from rock phosphate mine area Hazara Pakistan. Bacterial strains, specifically Sulphur oxidizing bacteria (SOB), have the ability to oxidize elemental sulphur (S°) and overcome S compounds to release sulphuric acid. Sulphuric acid converts the insoluble phosphorous (P) compounds to simple plant available P compounds. Best strains were screened out on the basis of morphological and biochemical characteristics, pH reduction test, sulphate ion production test, indole acetic production test. Among 30 isolates 10 were capable of reducing the pH of the growth media below 4.0 from initial pH 8.0 and made the highest sulphate production in the growth media and concentration of produced sulfate ion ranged from 1.73 to 2.0063mg/mL. On the basis of pH reduction (in thiosulphate broth) and phosphorous solubilization index (PSI) best strains were screened out. A total of ten selected SOB were tested in 0.5% tricalcium phosphate (TCP) broth media for the efficiency Phosphorous solubilization. Results showed that the strain Endo2 released 2.0435 mg L-1 P (95.2%) during 45 days of incubation shows Capacity of Phosphorus dissolved had a significant correlation revise this line. The bacterial isolates were identified as Bacillus spp., Serratia spp. and Enterobacter spp thiobacillus spp. based on their 16Srna, morphological and biochemical characterization.
Narendra R. 2018. a. Isolation and screening of drought and saline tolerant rhizospheric plant growth promoting bacteria from saline soils and their effect on growth and yield of ground NUT (Arachis hypogaea L.). Disscussion Acharya NG Ranga Agricultural University 8(6), 709.
Rodríguez H, Fraga R, Gonzalez T, Bashan Y. 2006. Genetics of phosphate solubilization and its potential applications for improving plant growth-promoting bacteria. Plant and soil 287(1-2), 15-21. http://dx.doi.org/10.1007/s11104-006-9056-9
Rehman OU, Sheikh AA, Gill KH. 2000. Available phosphorous and pH status of Attock soils. Pakistan Journal of Agricultural Sciences 37(1-2), 74-76.
Solangi M, Memon M, Puno HK. 2006. Assessment of phosphorus in soils of district Shikarpur, Pakistan. International Journal of Agriculture and Biology 4(1), 565-566.
Behera BC, Singdevsachan SK, Mishra RR, Dutta SK, Thatoi HN. 2014. Diversity, mechanism and biotechnology of phosphate solubilising microorganism in mangrove A review. Biocatalysis and Agricultural Biotechnology 3(2), 97-110. https://doi.org/10.1016/j.bcab.2013.09.008
Beijerinck MW. 1904. Phenomenes reduction proguits parles microbes. Archive Sciences 2(3), 9131-9157.
Vidyalakshmi R, Sridar R. 2007. Isolation and characterization of sulphur oxidizing bacteria. Journal of Culture Collections 5(1), 73-77.
Hassan SH, Van Ginkel SW. 2011. A novel biosensor for detecting toxicity in water using sulfur-oxidizing bacteria. Sensors and Actuators B: Chemical 154(1), 17-21.
Aria MM, Lakzian A, Haghnia GH, Berenji AR, Besharati H, Fotovat A. 2010. Effect of Thiobacillus, sulfur, and vermicompost on the water-soluble phosphorus of hard rock phosphate. Bioresource technology 101(2), 551-554. http://dx.doi.org/10.1016/j.biortech.2009.07.093.
Ullah I, Jilani G, Saifullah K, Khan M, Akhtar S, Rasheed M. 2014. Sulfur oxidizing bacteria from sulfur rich ecologies exhibit high capability of phosphorous solubilization. International Journal of Agriculture and Biology 16(3), 25-35.
Lee BH, Lee MJ, Park S, Oh DC, Elsasser S, Chen PC, Wilson SM. 2010. Enhancement of proteasome activity by a small-molecule inhibitor of USP14. Nature 467(7312), 179. http://dx.doi.org/10.1038/nature09299.
NareshKumar R, Nagendran R. 2008. Changes in nutrient profile of soil subjected to bioleaching for removal of heavy metals using Acidithiobacillus thiooxidans. Journal of Hazardous Materials 156(1-3), 102-107.
Bhatti TM, Yawar W. 2010. Bacterial solubilization of phosphorus from phosphate rock containing sulfur-mud. Hydrometallurgy 103(1-4), 54-59.
Jha CK, Aeron A, Patel BV, Maheshwari DK, Saraf M. 2011. Enterobacter: role in plant growth promotion. In Bacteria in agrobiology: Plant growth responses p 159-182. Springer, Berlin, Heidelberg.
Premono ME, Moawad AM, Vlek PLG. 1996. Effect of phosphate-solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere (No. REP-12113. CIMMYT.).
Islam MT, Deoraa A, Hashidokoa Y, Rahmana A, Itoa T, Taharaa S. 2007. Isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of Oryza sativa L. cv. BR29 of Bangladesh Natural sciences 62(3), 103-110.
Sharif M, Sarir MS, Rabi F. 2000. Biological and chemical transformation of phosphorus in some important soil series of NWFP. Sarhad Journal of Agriculture 16(1), 587-592.
Pokorna B, Mandl M, Borilova S, Ceskova P, Markova R, Janiczek O. 2007. Kinetic constant variability in bacterial oxidation of elemental sulfur. Applied Environomental Microbiology 73(2), 3752-3754.
Briand LE, Bonetto RD, Ladaga JL, Donati E. 1999. Bulk and surface characterization of crystalline and plastic sulphur oxidized by two Thiobacillus species. Process Biochemistry 34(1), 249-256.
Hariprasad P, Niranjana SR. 2009. Isolation and characterization of phosphate solubilizing rhizobacteria to improve plant health of tomato. Plant Soil 316(1), 13-24.
Chi R, Xiao C, Huang X, Wang C, Wu Y. 2007. Bio-decomposition of rock phosphate containing pyrites by Acidithiobacillus ferrooxidans. Journal of Central South University and technology 14(1), 170-175.
Sharif M, Sarir MS Rabi F. 2000. Biological and chemical transformation of phosphorus in some important soil series of NWFP. Sarhad Journal of Agriculture 16(1), 587-592.