Efficient plant regeneration from cotyledonary explants of Tunisian Cucumis melo L. cv. Maazoun and Beji
Paper Details
Efficient plant regeneration from cotyledonary explants of Tunisian Cucumis melo L. cv. Maazoun and Beji
Abstract
A procedure for the regeneration of melon (Cucumis melo L.) cv. Maazoun and Beji via shoot organogenesis from cotyledon explants is described. Cotyledons explants cultivated on the basal Murashige and Skoog (1962) medium, added with combinations of BAP, NAA and 2,4-D developed adventitious shoots after two weeks. The rates of caulogenesis varied according to the medium hormonal composition and the genotype. The best induction medium for a morphogenic response was MS containing 0.5 mgl-1 NAA and 2 mgl-1 BAP. The highest caulogenesis percentages were 79.16% and 58.33% for Maazoun and Beji cultivars respectively. The mean number of buds per callus was 2.92. Development phases were characterized by histological studies. Some phases of shoot organogenesis were observed in Cucumis melo L. Maazoun and Beji cultivars. The shoots were rooted in MS medium without growth regulators or added NAA (0.5 or 1 mgl-1). The highest bud rooting of buds occurred on MS medium added with 1 mg/l NAA. The percentage of rooting varied from 16.66 to 75%. The highest percentage of survival plants after acclimatization were 41.73 and 57.63 for Beji and Maazoun respectively.
Ananthakrishnan G, Xia X, Elman C, Singer S, Paris HS, Gal-On A, Gaba V. 2003. Shoot production in squash (Cucurbita Pepo) by in vitro organogenesis. Plant Cell Reports 21, 739-746.
Ben Ghnaya A, Boussaid M. 2002. In vitro tissue culture in snake melon (Cucumis melo L. var flexuosus): Induction to caulogenesis, neoformation of plants and analysis of the variability of regenerating: PhD, University of Sciences of Tunis, Tunisia, 84 pages.
Chovelon V, Restier V, Dogimont C, Aarrouf J. 2008. Histological study of shoot organogenesis in melon (Cucumis melo L.) after genetic transformation. Cucurbitaceae 2008, Proceedings of the IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae (Pitrat M, ed), INRA, Avignon (France).
Compton ME, Gray DJ. 1993. Shoot organogenesis and plant regeneration from cotyledons of diploid, triploid, and tetraploid watermelon. Journal of the American Society for Horticultural Science 118, 151-157.
Dabauza M. 1995. Selection and characterization of somatic hybrids Cucumis melo L. Anguria Cucumis L. var. longipes, Cucumis melo L. Myriocarpus Naud and Cucumis Cucumis melo L. Citrullus colocynthis (L) Schard. PhD in Biological Sciences, ETSIA Polytechnic University of Valencia, Spain, 272 ff.
Dagnelie P. 1974. Theory and statistical methods. Agricultural Applications, (2nd ed) Methods of statistical inference, Vol 1 (2463p). The Agricultural Press Gembloux, Belgium
Daimon H, Ohno H, Akasaka Y, Mii M. 2002. A histological evaluation of adventitious bud formation in cotyledons in Crotalaria juncea L. Plant Production Science 5, 301-304.
DG / PIDA. Ministry of Agriculture of Tunisia. 1999. Survey results on the monitoring of the crop year 1997/1998 – General Directorate of Agricultural Production Department of Agriculture 1997, 1998-33 pages.
Gaba V, Schlarman E, Elman C, Sagee O, Watad A, Gray DJ. 1999. In vitro studies on the anatomy and morphology of bud regeneration in melon cotyledons. In Vitro Cellular and Developmental Biology – Plant 35, 1-7.
Gambley RL, Dodd WA. 1991. The influence of cotyledons in axillary and adventious shoot production from cotyledonary nodes of Cucumis sativus L. (cucumber). Journal of Experimental Botany 42, 1131-1135.
Gui M, Ben Amor M, Latché A, Pech JC, Roustan P. 2000. A reliable system for the transformation of cantaloupe charentais melon (Cucumis melo L. var. cantalupensis) leading to a majority of diploid regenerants. Scientia Horticulturae 84, 91-99.
Halit Y, Nebahat S. 2003. A new method for haploid muskmelon (Cucumis melo L.) dihaploidization. Scientia Horticulturae 98, 277-283.
Han JS, Oh DG, Mok IG, Park HG, Kim CK. 2004. Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.). Plant Cell Reports 23, 291-296.
Hernandez-Gomes LF, Ubeda-Iranzo J, Garcia-Romero E, Briones-Pérez A. 2005. Comparative production of different melon distillates: chemical and sensory analyses. Food Chemistry 90, 115-125.
Hnana M, Harrabi M, Boussaid M. 2004. Induction of caulogenesis and somatic embryos in Cucumis melo (var. Flexuosus). Journal of Applied Horticulture 4, 77-82.
Zhang H, Peng G, Feishi L. 2011. Efficient plant regeneration from cotyledonary node explants of Cucumis melo L. African Journal of Biotechnology 10, 6757-6761.
Jebberi H. 1988. Melon. Technical documents. Ann. Inst. Rech. Agro. Tunisia, 104 36.
Chengalrayan K, Hazra S, Gallo-Meagher M. 2001. Histological analysis of somatic embryogenesis and organogenesis induced from mature zygotic embryo-derived leaflets of peanut (Arachis hypogaea L.). Plant Science 161, 415–421
Kageyama K, Yabe K, Miyajima S. 1990. Somatic embryogenesis and plant regeneration from stem, leaf, and shoot apex of melon (Cucumis melo L.). Plant Tissue Culture 7, 193-198.
Kathiravan K, Vengedesan G, Singer S, Steinitz B, Paris H, Gaba V. 2006. Adventitious regeneration in vitro occurs across a wide spectrum of squash (Cucurbita pepo) genotypes. Plant Cell, Tissue and Organ Culture 85, 285-295.
Ledo A, DA S, Lameira OA, Benbadis AK, De Menezes IC, De Oliveira M DS O, Filho SM. 2002. Somatic embryogenesis from zygotic embryos of Euterpe oleracea Mart. Jaboticabal- SP, Revista Brasileira de Fruticultura 24, 601-603.
Lee YK, Chung WI, Ezura H. 2003. Efficient plant regeneration via organogenesis in winter squash (Cucurbita maxima Duch). Plant Science 164, 413-418.
Mezghani N, Jemmali A, Elloumi N, Gargouri-BR, Kintzios S. 2007. Morpho-histological study on shoot bud regeneration in cotyledon cultures of pepper (Capsicum annuum). Biologia, Bratislava 62, 704-710.
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bio-assays with tabacco tissues cultures. Journal of Plant Physiology 15, 473–497.
Rhimi A, Boussaid M. 2012. An efficient method for production of Tunisian melon (Cucumis melo L. cultivars Maazoun and Beji) by somatic embryogenesis. African Journal of Biotechnology 11, 9924-9931.
Rhimi A, Ben Fadhel N, Boussaid M. 2006. Plant regeneration via somatic embryogenesis from in vitro tissue culture in two Tunisian Cucumis melo cultivars Maazoun and Beji. Plant Cell Tissue and Organ Culture 84, 239-243.
Rhimi A, Boussaid M. 2000. In vitro tissue culture of melon (Cucumis melo L.) cv Maazoun: caulogenesis and somatic embryogenesis induction in this species. PhD, University of Sciences of Tunis, Tunisia, 71 pages.
Ntui VO, Sher khan R, Chin DP, Nakamura I, Mii M. 2010. An efficient Agrobacterium tumefaciens-mediated genetic transformation of ‘‘Egusi’’ melon (Colocynthis citrullus L.). Plant Cell Tissue and Organ Culture 103, 15-22.
Von Arnold S, Sabala I, Bozhkov P, Dyachok J, Filonova L. 2002. Important aspects of somatic embryogenesis. Plant Cell Tissue and Organ Culture 69, 233-249.
Yalçin MY. 2003. Effect of cotyledon age and explant location on regeneration of Cucumis sativus. Biotechnology & Biotechnological Equipment 1, 38-43.
Rhimi Awatef, Boussaid Mohamed (2013), Efficient plant regeneration from cotyledonary explants of Tunisian Cucumis melo L. cv. Maazoun and Beji; JBES, V3, N12, December, P50-58
https://innspub.net/efficient-plant-regeneration-from-cotyledonary-explants-of-tunisian-cucumis-melo-l-cv-maazoun-and-beji/
Copyright © 2013
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0