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Elucidation of the antagonistic effect of Bacillus species against white mold fungus Sclerotinia sclerotiorum

Research Paper | October 1, 2018

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Afsana Hossain, Rakibul Hasan, Md. Monirul Islam, Juel Datta, Md. Mahidul Islam Masum

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Int. J. Biosci.13( 4), 195-207, October 2018

DOI: http://dx.doi.org/10.12692/ijb/13.4.195-207


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Sclerotinia sclerotiorum is one of the most devastating and cosmopolitan soil-borne fungus and causes white mold diseases on several economically important crops, such as oilseed rape, soybean, cotton and numerous vegetable crops. The disease has been controlled primarily through the use of several chemicals; however, the fungicides are often ineffective and not environmentally safe. The use of Bacillus species has attracted much attention because of their biocontrol potential for combating plant fungal diseases. The aims of this study were therefore to evaluate the antifungal activity of Bacillus species and to elucidate the underlying inhibitory mechanism against S. sclerotiorum. The inhibitory effect of Bacillus species on S. sclerotiorum was screened by agar diffusion assay. In dual culture agar diffusion experiment, strong antifungal activity was observed against mycelial growth of S. sclerotiorum by four isolates of Bacillus, while Bacillus amyloliquefaciens isolate A1 showed highest antifungal activity (67%). Moreover, cell free culture filtrate of isolate A1 exhibited strong mycelial growth inhibition of fungus (77.5%). Microscopic studies revealed the morphological alteration of fungal hyphae after treated with isolate A1. The abilities of biofilm, siderophore and lip peptides production were also investigated to clarify the underlying inhibitory mechanism of the most effective strains. Results indicated that isolate A1 showed prolific production of biofilm (0.2129 0f OD570) and strong siderophore activity (1.69 cm) as compared to other Bacillus isolates. Moreover, three metabolites groups namely iturin, fengycin and surfactin were detected in the single cell of isolate A1 using MALDI-TOF-MS spectrum analysis. Taken together, these results demonstrated that B. amyloliquefaciens isolate A1 may have potential as an effective biocontrol agent for fighting against white mold fungus S. sclerotiorum.


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Elucidation of the antagonistic effect of Bacillus species against white mold fungus Sclerotinia sclerotiorum

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