Embryonic blood vessel damage and embryotoxicity induced by electronic cigarettes vape juice

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Research Paper 05/08/2023
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Embryonic blood vessel damage and embryotoxicity induced by electronic cigarettes vape juice

Menrie L. Arabos, Sharmilah P. Bautista, Jerome V. Domondon, Princess Faye W. Osting, Beatriz E. Aspiras
Int. J. Biosci. 23(2), 169-176, August 2023.
Copyright Statement: Copyright 2023; The Author(s).
License: CC BY-NC 4.0

Abstract

This study is an investigation of electronic cigarette’s vape juice for its embryonic blood vessel damage and embryotoxicity by the use of Chorioallantoic Membrane Assay. Assessment of vascular areas showed lysis, hemorrhage, coagulation, and hyperemia in the pure grade nicotine treatment, nicotine-containing vape juice experimental treatments, and non-nicotine-containing vape juice treatments. Mean weight, mean length, percentage of dead embryos, and percentage of dysmorphogenic embryos were used as parameters to evaluate embryotoxicity. Anas platyrhynchos eggs were used as test organism. Independent Samples Kruskal-Wallis revealed that there is a significant difference in the mean weight and mean lengths of Anas platyrhynchos embryos. The percentage of dead embryos conveys possible effect of various concentrations of vape juice treatments in the death of embryos. Dysmorphogenesis in embryos was recorded in treatments containing nicotine. Results of this study can lay foundations in the areas of public awareness, consumption, education, research, and healthcare.

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