Enhanced yeast growth and bioethanol production using molasses and fermentation medium as substrates

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Enhanced yeast growth and bioethanol production using molasses and fermentation medium as substrates

Shafique Ahmed Rind, Fateh Muhammad Soomro, Agha Asad Noor, Atta Hussain Rind
Int. J. Biosci.16( 1), 455-464, January 2020.
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The ethanol derived by the use of microorganisms during industrial fermentation process has been used as an alternate source of liquid energy and a substitute of high-cost petrol. Saccharomyces cerevisiae is best known microbial source due to its major impact on economic and social importance, rapid conversion of sugar, high growth and fermentation rate, high genetic stability and tolerance in stressful conditions. This work revealed that maximum growth of test strain on WL-nutrient broth, 0.8 ml inoculum size, 5.0 pH, and 32°C at 120 rpm after 72 hours. Glucose, sucrose; arginine, asparagine, glutamic acid; pantothenic acid, thiamine and riboflavin were the best organic sources for enhanced growth of test strains whereas calcium chloride, magnesium chloride, potassium chloride, ammonium chloride, ammonium sulphate, magnesium sulfate, manganese sulphate, potassium di-hydrogen phosphate, di-Potassium hydrogen phosphate, urea, peptone, yeast nitrogen base were the best chemicals for increased growth of test strain at their respective concentrations per 100ml. of WL-nutrient broth. The effect of alcohols showed that the test strain Cs tolerated higher concentration of ethanol as compared to the methanol and isopropanol. When studied the comparative growth, it was observed that the synthetic growth medium showed increased growth as compared to other media used. Ethanol production from clarified molasses and mixed with growth medium at different concentrations revealed 24.91% and 15.7% at sugar concentration 44 and 35.5% and sugar consumption 33.26 and 26.61% respectively.


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