Enhancement of commercial detergent’s wash performance by addition of enzymes of Bacillus subtilis FH1

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Research Paper 01/02/2020
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Enhancement of commercial detergent’s wash performance by addition of enzymes of Bacillus subtilis FH1

Muhammad Aamir Khan, Muhammad Imran, Abdul Hameed, Fariha Hasan
Int. J. Biosci.16( 2), 7-11, February 2020.
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Abstract

Currently enzymatic detergents emerged as better option due to their cleaning and stain removal effectiveness in comparison to the synthetic surfactants. An extracellular alkaline protease produced by Bacillus subtilis strain FH1 was studied for its potential application as detergent additive. Enzyme was tested for its compatibility and stability by incubating it with several detergents like Surf Excel, Ariel, Express, Bonus and Brite and residual activity was checked by standard enzyme assay, it showed 92, 85, 100, 75 and 126% residual activity respectively. Its wash performance was checked by adding in these detergents while washing manually soiled cotton cloth pieces and showed excellent wash performance in combination with detergents at lower temperature. Due to stability and good wash performance the enzyme can be used as detergent additive.

VIEWS 19

Beg QK, Gupta R. 2003. Purification and Characterization of an Oxidation-stable, Thiol-dependent Serine Alkaline Protease from Bacillus mojavensis. Enzyme and Microbial Technology 32(2), 294-304. https://doi.org/10.1016/S0141-0229(02)00293-4

Gupta R, Beg QK, Lorenz P. 2002. Bacterial alkaline proteases: molecular approaches and industrial applications. Applied Microbiology and Biotechnology 59(1), 15-32. https://doi.org/10.1007/s00253-002-0975-y

Gupta R, Gupta K, Saxena R, Khan S. 1999. Bleach-stable, alkaline protease from Bacillus sp. Biotechnology Letters 21(2), 135-138. https://doi.org/10.1023/A:1005478117918

Jurado E, Bravo V, Luzon G, Fernandez-Serrano M, Garcia-Roman M, AltmajerVaz D, Vicaria JM. 2007. Hard-surface cleaning using lipases: enzyme–surfactant interactions and washing tests. Journal of Surfactants and Detergents 10(1), 61-70. https://doi.org/10.1007/s11743-006-1009-z

Kembhavi AA, Kulkarni A, Pant A. 1993. Salt-tolerant and thermostable alkaline protease from Bacillus subtilis NCIM No. 64. Applied Biochemistry and Biotechnology 38(1-2), 83-92. https://doi.org/10.1007/BF02916414

Kirk O, Borchert TV, Fuglsang CC. 2002. Industrial Enzyme Applications. Current Opinion in Biotechnology 13(4), 345-351. https://doi.org/10.1016/S0958-1669(02)00328-2

Kitayama M. 1992. New low-temperature alkaline protease. Japanese Patent no. JP4271781.

Kumar CG, Takagi H. 1999. Microbial alkaline proteases from a bioindustrial viewpoint. Biotechnology Advances 17(7), 561-594. https://doi.org/10.1016/S0734-9750(99)00027-0

Lageiro MM, Moura MJ, Reis A, Costa-Ferreira MJ. 2007. Microbial Proteases Application in Leather Industry. Journal of Biotechnology 2(131), S239-S240. http://dx.doi.org/10.1016%2Fj.jbiotec.2007.07.717

Mitidieri S, Martinelli AHS, Schrank A, Vainstein MH. 2006. Enzymatic detergent formulation containing amylase from Aspergillus niger: a comparative study with commercial detergent formulations. Bioresource Technology 97(10), 1217–1224. https://doi.org/10.1016/j.biortech.2005.05.022

Natt MA. 2000. Studies on the production and use of microbial enzymes in leather processing (Doctoral Dissertation). Quaid-i-Azam University, Islamabad, Pakistan.

Priest FG. 1977. Extracellular enzyme synthesis in the genus Bacillus. Bacteriological Reviews 41, 711-753.

Rai SK, Konwarh R, Mukherjee AK. 2009. Purification, characterization and biotechnological application of an alkaline β-keratinase produced by Bacillus subtilis RM-01 in solid-state fermentation using chicken-feather as substrate. Biochemical Engineering Journal 45(3), 218-225. https://doi.org/10.1016/j.bej.2009.04.001

Tamiya E, Nakamura T. 1996. Protease active at low temperature. Patent no. JP8080190.