Enhancement of commercial detergent’s wash performance by addition of enzymes of Bacillus subtilis FH1

Paper Details

Research Paper 01/02/2020
Views (975)
current_issue_feature_image
publication_file

Enhancement of commercial detergent’s wash performance by addition of enzymes of Bacillus subtilis FH1

Muhammad Aamir Khan, Muhammad Imran, Abdul Hameed, Fariha Hasan
Int. J. Biosci. 16(2), 7-11, February 2020.
Copyright Statement: Copyright 2020; The Author(s).
License: CC BY-NC 4.0

Abstract

Currently enzymatic detergents emerged as better option due to their cleaning and stain removal effectiveness in comparison to the synthetic surfactants. An extracellular alkaline protease produced by Bacillus subtilis strain FH1 was studied for its potential application as detergent additive. Enzyme was tested for its compatibility and stability by incubating it with several detergents like Surf Excel, Ariel, Express, Bonus and Brite and residual activity was checked by standard enzyme assay, it showed 92, 85, 100, 75 and 126% residual activity respectively. Its wash performance was checked by adding in these detergents while washing manually soiled cotton cloth pieces and showed excellent wash performance in combination with detergents at lower temperature. Due to stability and good wash performance the enzyme can be used as detergent additive.

Beg QK, Gupta R. 2003. Purification and Characterization of an Oxidation-stable, Thiol-dependent Serine Alkaline Protease from Bacillus mojavensis. Enzyme and Microbial Technology 32(2), 294-304. https://doi.org/10.1016/S0141-0229(02)00293-4

Gupta R, Beg QK, Lorenz P. 2002. Bacterial alkaline proteases: molecular approaches and industrial applications. Applied Microbiology and Biotechnology 59(1), 15-32. https://doi.org/10.1007/s00253-002-0975-y

Gupta R, Gupta K, Saxena R, Khan S. 1999. Bleach-stable, alkaline protease from Bacillus sp. Biotechnology Letters 21(2), 135-138. https://doi.org/10.1023/A:1005478117918

Jurado E, Bravo V, Luzon G, Fernandez-Serrano M, Garcia-Roman M, AltmajerVaz D, Vicaria JM. 2007. Hard-surface cleaning using lipases: enzyme–surfactant interactions and washing tests. Journal of Surfactants and Detergents 10(1), 61-70. https://doi.org/10.1007/s11743-006-1009-z

Kembhavi AA, Kulkarni A, Pant A. 1993. Salt-tolerant and thermostable alkaline protease from Bacillus subtilis NCIM No. 64. Applied Biochemistry and Biotechnology 38(1-2), 83-92. https://doi.org/10.1007/BF02916414

Kirk O, Borchert TV, Fuglsang CC. 2002. Industrial Enzyme Applications. Current Opinion in Biotechnology 13(4), 345-351. https://doi.org/10.1016/S0958-1669(02)00328-2

Kitayama M. 1992. New low-temperature alkaline protease. Japanese Patent no. JP4271781.

Kumar CG, Takagi H. 1999. Microbial alkaline proteases from a bioindustrial viewpoint. Biotechnology Advances 17(7), 561-594. https://doi.org/10.1016/S0734-9750(99)00027-0

Lageiro MM, Moura MJ, Reis A, Costa-Ferreira MJ. 2007. Microbial Proteases Application in Leather Industry. Journal of Biotechnology 2(131), S239-S240. http://dx.doi.org/10.1016%2Fj.jbiotec.2007.07.717

Mitidieri S, Martinelli AHS, Schrank A, Vainstein MH. 2006. Enzymatic detergent formulation containing amylase from Aspergillus niger: a comparative study with commercial detergent formulations. Bioresource Technology 97(10), 1217–1224. https://doi.org/10.1016/j.biortech.2005.05.022

Natt MA. 2000. Studies on the production and use of microbial enzymes in leather processing (Doctoral Dissertation). Quaid-i-Azam University, Islamabad, Pakistan.

Priest FG. 1977. Extracellular enzyme synthesis in the genus Bacillus. Bacteriological Reviews 41, 711-753.

Rai SK, Konwarh R, Mukherjee AK. 2009. Purification, characterization and biotechnological application of an alkaline β-keratinase produced by Bacillus subtilis RM-01 in solid-state fermentation using chicken-feather as substrate. Biochemical Engineering Journal 45(3), 218-225. https://doi.org/10.1016/j.bej.2009.04.001

Tamiya E, Nakamura T. 1996. Protease active at low temperature. Patent no. JP8080190.

Related Articles

Anti-proliferative potential of seed derived proteins from Vitis vinifera and Mangifera indica

Hareeshthulasi, V. Vinotha, R. Rajakumar*, Int. J. Biosci. 28(4), 129-137, April 2026.

Valorisation of table waste and fruit waste by black soldiers (Ullicens hermetica)

Ayaba Adéline Hounnou, Vanessa Chabi, Jomini Marc Sène Alitonou, Franck Sokenou, Mickael Vitus Martin Kpessou Saïzonou, Fidèle Paul Tchobo, Guy Alain Alitonou*, Int. J. Biosci. 28(4), 123-128, April 2026.

Murraya koenigii (Linn.) Spreng.: An opulent source of fatty acid

Shahin Aziz*, Int. J. Biosci. 28(4), 116-122, April 2026.

Design and architecture of an IoT-enabled bamboo resource management system: Data-driven approach for sustainable agriculture

Charlot L. Maramag*, Dorothy M. Ayuyang, Richard R. Ayuyang, Int. J. Biosci. 28(4), 107-115, April 2026.

Physicochemical and microbiological characterization of flours from the local variety of purple corn (Zea mays L.) produced and marketed in Katiola (Côte d’Ivoire)

Moumouny Traore*, N´Zebo Desiré Kouame, Pepiesin Marie Ange Melem Soro, Zamblé Bi Irié Abel Boli, Int. J. Biosci. 28(4), 98-106, April 2026.

In the shadows of governance: Exploring youth participation in local peacebuilding initiatives

Juramie R. Rubia, Benny R. Rubia, Nancy E. Aranjuez*, Int. J. Biosci. 28(4), 85-97, April 2026.

Evaluation of the agronomic performance, beta-carotene content and dry matter content of 228 sweet potatoes (Ipomoea batatas (L.) Lam) genotypes in Burkina Faso

Nattan Gamsore*, Koussao Some, Djakaridja Tiama, Pauline Bationo_Kando, Int. J. Biosci. 28(4), 73-84, April 2026.

Geomatics tools for agricultural and farm disaster risk management and reduction: A survey of farmers in South-South coastal communities, Nigeria

G. O. Nwodo, O. J. Ugwu, E. U. Onah, A. Ugwuoti, E. Elijah Ebinne*, O. P. Nogheghase, S. I. Ogbaa, U. E. Ahuchaogu, T. O. Ogbuji, C. P. Owuamalam-Chidi, C. O. Osuagwu, M. O. Igwenagu, O. E. Mbakaogu, J. U. Chikaire, Int. J. Biosci. 28(4), 59-72, April 2026.