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Research Paper | November 20, 2022

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Enhancement of phosphate solubilization by Bacillus subtilis induced by UV mutagenesis

Chibani Hiba Rahman, Hamoum Hakim, Chibani Abdelwaheb

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Int. J. Biosci.21(5), 239-245, November 2022

DOI: http://dx.doi.org/10.12692/ijb/21.5.239-245

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Abstract

The ability of PSB to solubilize phosphate is considered to be one of the most important traits in plant nutrition. These bacteria play an important role in providing plants with phosphorus. The aim of the present study is the isolation of phosphate solubilizing bacteria as well as the enhancement of phosphate solubilization of Bacillus subtilis using UV mutagenesis. 24 bacteria were isolated from the rhizosphere of different regions in a western of Algeria on the basis of their ability to solubilize tricalcium phosphate in on Pikovskaya’s agar plates. Using liquid medium significant amounts of phosphorus solubilized by the isolates were recorded, reaching a value of 346.34μg/ml of free phosphorus by the isolate GC9. This later was subjected to UV mutagenesis for its high phosphate solubilizing ability and it was identified as Bacillus subtilis using 16S rRNA gene sequence analysis. Induction of mutation in strain was carried out at different exposure times: 0, 4, 8, 12 and 16 min at a distance of 10 cm between UV source and treated bacteria. A high rate of improvement of phosphate solubilization in liquid medium was observed by the mutant GCM11 (109.78%).

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Enhancement of phosphate solubilization by Bacillus subtilis induced by UV mutagenesis

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