Entomopathogenic efficacy of Beauvaria bassina and Bacillus thurengiensis against aphid (Aphis gossypii) Glover on okra under field conditions

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Research Paper 01/06/2021
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Entomopathogenic efficacy of Beauvaria bassina and Bacillus thurengiensis against aphid (Aphis gossypii) Glover on okra under field conditions

Sarah Allah Dada, Javed Khan, Bashir Ahmad Khan, Imtiaz Khan, Aisha Irshad, Rafia Saif, Ali Waqar
Int. J. Biosci.18( 6), 268-272, June 2021.
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A field trial was undertaken to study the harmful effect of entomopathogenic Beauvaria bassina and Bacillus thureingienis for the management of Aphis gossypii aphid on okra under field condition. Three replications for each treatment were used. The rate of concentrations was (108 – 109 conidiamL-1) of Beauveria bassiana and (108- 109sporesmL-1) Bacillus thureingiensis were applied as foliar spray. the significant maximum mean reduction in the population of aphids was recoded for Beauveria + Bacillus 30.59, 63.55 and 84.85 followed by Beauveria bassiana with% reduction of 27.86,57.52 and 79.20, and 26.54, 56.88 and 77.56 after 24, 48 and 72 hrs of post application respectively. The minimum% reduction in population of aphids was recorded from control with 6.55, 8.50±0.36 and 9.75 after 24, 48 and 72 hrs post treatments application. The combination of Beauvaria bassina and Bacillus thureingienis caused maximum% reduction in population in the field therefore, it recommended to use biopesticdes for the management of A. gossypii in okra field.


Aronson A. 2002. Sporulation and d-endotoxin synthesis by Bacillus thuringiensis. Cell. Mol. Life Sci 59, 417-425.

Aronson AI. 1993. The two faces of Bacillus thuringiensis insecticidal proteins and post-exponential survival. Mol. Microbiol 7, 489-496.

Athanassiou CG, Kavallieratos NG, Vayias BJ, Tsakiri JB, Mikeli Meletsiscm NH. 2008. Persistence and efficacy of Metarhizium anisopliae (Metschnikoff) Sorokin (Deuteromycotina: Hyphomycetes) and diatomaceous earth against Sitophilus oryzae (L.) (Coleoptera: Bostrychidae) on wheat and maize. Crop Protection 27, 1303-1311.

Bayer C, Kubitzki K. 2003. The families and genera of vascular plants. In: Malvaceae, (Ed.) Crop Protec 30, 225-311.

Carruthers RI. Hural K. 1990. Fungi as naturally occurring entomopathogens. UCLA Symp. Mol. Cell. Biol. (USA) 112, 115-138.

Chinniah C, Ravikumar A, Kalyanasundaram M, Parthiban P. 2016. Management of suckin pests, by integration of organic sources of amendments and foliar application of entomopathogneic fungi on chilli. J. Biopesticids 9(1), 34-40.

Erler F, Ozgur AA. 2015. Potential of two entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Coleoptera: Scarabaeidae), as biological control agents against the June beetle. J of Insect Scie 15(1), 44-50.

Feitelson J. 1993. The Bacillus thuringiensis family tree. In: Kim L (ed) Advanced Engineered Pesticides, Marcel Dekker, Inc.New York, N.Y pp. 63-72.

Janaki I, Suresh S, Karuppuchamy PT. 2010. Efficacy and economics os biopesticides for the management of papaya mealybug, Paracoccus marginatus (Williams and Granara de Willink) in brinjal (Solanum melongena L). J. Biopesticides 5(1), 87-90.

Jugno TQ, Waheed H, Nawaz BH, Muhammad S, Talha N, Tauqir A, Abdul H. 2018. Potential assessment of Metarhizium anisopliae and Bacillus thuringiensis against Brinjal insect pests Amrasca bigutulla (Jassid) and Aphis gossypii (Aphid). Journal of Entomol and Zool Studies 6(2), 32-36.

Latge JP, Papierok B, Minks AK, Harrewijin P. 1988. Aphids their biology, natural enemies and control. Vol 2B. Elsevier, Amsterdam, Netherlands pp. 323-335.

Messing RH, Tremblay MN, Mondor EB, Foottit RG, Pike KS. 2007. Invasive aphids attack native Hawaiian plants. Bio Invasions 9, 601-607.

Sandhu SS, Sharma AK, Beniwal V, Goel G, Batra P, Kumar A. 2012. Myco-Biocontrol of Insect Pests factors involved, mechanism and regulation. J of pathogens 1-10.

Sarkar PK, Mukherjee AB, Ghosh J. 1996. Assessment of loss of bhindi against red spider mite. Environ. Ecolo 14(2), 480-481.

Shafighi Y, Ziaee M, Ghosta Y. 2014. Diatomaceous earth used against insect pests applied alone or in combination with Metarhizium anisopliae and Beauveria bassiana. J. Plant Protec. 54, 62-66.

Shahzad AS, Ahmad S.S, Rizwan H, Siddiqui SS, Ali S. 2016. Effect of Biopesticides against sucking insect pests of brinjal crop under field conditions. J. basic and applied sci 12, 41-49.

Sharma JH, Anoorag RT. 2017. Evaluation of Bio-rational pesticides, against brinjal fruit shoot borer, Leucinodes orbonalis Guen on brinjal at Allahabad Agroclimatic region. Int. J Current Microbio and Appli Scie 6(6), 2049-2054.

Sirinivasan R. 2012. Integrating biopesticides in pest management strategies for tropical vegetable production. J of Biopesticides 36-45.

Sung GH, Hywel-Jones NL, Sung J, Luangsa-ard JJ, Shrestha B, Spatafora JW. 2007. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Studies Mycol 57, 5-59.

Ursani TJ, Malik S, Chandio JI, Palh ZA, Soomro MN, Lashari KH. 2014. Screening of biopesticides aginst insect pests of brinjal. Int. J. Emerging Scie and Tec (6), 918-931.

Van-Frankenhuyzen K. 2009. Insecticidal activity of Bacillus thuringiensis crystalproteins. J. Invertebr. Pathol 101, 1-16.

Wood BW, Tedders WL, Thompson JM. 1985. Feeding influence of three pecan aphid species on carbon exchange and phloem integrity of seedling pecan. J. American Society of Horti. Sci 110, 393-397.

Wu D, He J, Gong Y, Chen D, Zhu X, Qiu N. 2011. Proteomic analysis reveals the strategies of Bacillus thuringiensis YBT-1520 for survival under long-term heat stress. Proteomics. 2011; 11, 1-12.

Zamani AA, Talebi A, Fathipour Y, Baniameri V. 2006. Effect of temperature on biology and population growth parameters of Aphis gossypii Glover (Hom, Aphididae) on greenhouse cucumber. J. Applied Entomol 130, 453-460.