Essential oil composition of lemon verbena (Lippia citriodora) leaves cultivated in Mazandaran, Iran

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Essential oil composition of lemon verbena (Lippia citriodora) leaves cultivated in Mazandaran, Iran

Hojat Taheri Aziz Abadi, Nad Ali Bagheri, Nad Ali Babaeian Jelodar, Salman Sharifi Mehr
J. Bio. Env. Sci.4( 1), 135-140, January 2014.
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In this study the chemical composition of the essential oil extracted from leaves of Lippia citriodora (Verbenaceae) grown under greenhouse conditions in mazandaran, Iran. The essential oil was obtained by hydro-distillation method using Clevenger apparatus. The oil was analyzed by GC and GC-MS. Thirty-four compounds were identified. The main constituents of the oils in this method were sabinene (1.34%), 6 methyl-5heptene-2one (3.46%), D-limonene (5.81%), 1,8-Cineole (2.51%), trans-beta ocimene (1.17%), Alpha terpineol (1.75%), neral (12.6%), geranial(15.07%), geranial acetate (1.15%), caryophyllene (4.02), D-germacrene (3.52), alpha-curcumene (4.17%), bicyclogermacrene (3.42%), nerolidol (1.59%), spathulenol (4.40%) caryophyllene oxide (2.04%), alpha-cadinol (1.06%).In our study citral was also identified at high percentages.


Ballakhdar J, Idrissi AL, Canigueral S, Iglesias J, Vila R. 1994. Composition of lemon verbena (Aloysia triphylla (L’Herit) Britton) oil of Moroccan origin. Journal of Essential Oil Research 6(5), 523-526.

Catalan CAN, De Lampasona MEP. 2002. The Chemistry of the genus Lippia (Verbenaceae). In S.E. Kintzios, (ed.) Oregano: The genera origanum and Lippia, Taylor and Francis; London, 127–149.

Carnat A, Carnat AP, Fraisse D, Lamaison JL. 1999. Fitoterapia 70, 44

De Figueiredo R, Stefanini M, Ortiz Maio Marques M. 2004. Essential oil composition of Aloysia triphylla (L’Herit) Britton leaves cultivated in Botucatu, São Paulo, Brazil. Journal Medicinal and Aromatic Plants, 131-134.

Hanaa FMA, Beltagi HS, Nasr NF. 2008. Assessment of volatile components, free radical-scavenging capacity and anti-microbial activity of Lemon Verbena leaves. Research Journal of Phytochemistry 2(2) , 84-92.

Jones FA. 1996. Herbs-useful plants, their role in history and today. European Journal of Gastroenterology and Hepatology 8, 1227-1231.

Khani A, Basavand F, Rakhshani E. 2012. Chemical composition and insecticide activity of lemon verbena essential oil. Journal of Crop Protection 6(4), 313-320.

Kuwahara Y, Suzuki H, Matsumoto K , Wada Y. 1983. Pheromone study on acarid mites. XI. Function of mite body as geometrical isomerization and reduction of citral (the alarm pheromone) Carpoglyphus lactis. Appl. Entomol. Zool 18, 30–39.

Meshkatalsadat MH, Papzan AH, Abdollahi A. 2010. Determination of bioactive volatile organic components of Lippia citriodora using ultrasonic assisted with headspace solid phase microextraction coupled with GC-MS. Digest Journal of Nanomaterials and Biostructures 6(1), 319-323.

Moghtader M. 2009. Chemical composition of the essential oil of Teucrium polium L. from iran. American-Eurasian Journal of Agricultural & Environmental Sciences 5 (6), 843-846.

Ozek T, Kirimer N, Baser KHC, Tumen G. 1996. Composition of the essential oil of Aloysia triphilla (L’Herit) Britton grown in Turkey. Journal of Essential Oil Research 8(5), 581-583.

Onawunmi GO. 1989. Evaluation of the antimicrobial activity of citral. Lett. Appl. Microbial 9(3), 105–108.

Pawlisyzn J. 2007. Solid phase microextraction: Theory and practice, WilleyVCH, New York.

Rosmarinblätter-Rosmarini F. 2008. In: Europäisches Arzneibuch (European Pharmacopoeia). 6th ed. Volume 3.Monographien K-Z. Vienna: VerlagÖsterreich, 3865–3866.

Robacker DC, Hendry LB. 1977. Neral and geranial: components of the sex pheromone of the parasitic wasp, Itoplectis conquisitor. Journal of Chemical Ecology 3(5), 563-577. 10.1007/BF00989077

Santos-Gomes PC, Fernandes- Ferreira M, Vicente AMS. 2005. Journal Essent. Oil Research 17, 73.

Tutin TG. 1981. Lippia, Flora Europea. Cambridge University Press. P. 123.

C TERBLANCHÉ F, KORNELIUS G. 1996. Essential oil constituents of the genus Lippia (Verbenaceae) – A literature review. Journal of Essential Oil Research 8, 471-485. 10.1080/10412905.1996.9700673

Ahmed Mahmoud HJ, Scott AI, Reibenspies JH, Mabry T J. 1993. New sesquiterpene a-methylene lactones from the Egyptian plant Jasoniacandicans. Journal of Natural Prodaction 56, 1276–1280. 10.1021/np50098a011

Zygadlo JA, Lamarque AL, Maestri DM, Guzmán CA, Lucini EI, Grosso NR, Ariza L. 1994. Espinar, Journal Essential Oil Research 6, 407.