Estimating the microbial attributes and proximal composition of zn-aided bar

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Research Paper 01/04/2020
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Estimating the microbial attributes and proximal composition of zn-aided bar

Ayesha Rafique, Sarfraz Hussain, Shahid Mahmood, Tusneem Kausar, Ghulam Mueen-ud-Din, Syeda Mahvish Zahra, Sadia Munir, Muhammad Yousaf Quddoos, Muhammad Zia Shahid, Tabussam Tufail
Int. J. Biosci.16( 4), 159-173, April 2020.
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To overcome the malnutrition of micronutrients, specifically in children is the most influential topic now a days. Many interventions has been considered till now but oral dietary interventions has indispensable role to cope micronutrient deficiencies like Zn, which is essential for the rapid growth of adolescent boys. This study assimilated the potential of natural sources to mitigate Zn deficiency among zinc deficient boys through fortified food bars. By following different ratios of natural zinc fortificants i.e. pumpkin and sesame seed, treatments were prepared with oats, milk powder, sugar and butter. After preparation, bars were examined regarding estimation of zinc, proximate and microbial analysis at an equal interval of 15 days for sixty days.T0 is placebo, T1 is synthetic fortified bar and T2 is natural fortified bar. Natural zinc fortificants i.e. pumpkin and sesame seeds have 7.86 and 7.14 zinc. Excluding moisture, all elements of proximate composition were non-significant among storage interval of 2 months. In all treatments moisture ranged from 8.23 to 4.56 %, fat ranged from 17.99 to 27.9 %, protein ranged from 7.20 to 16.52, ash ranged from 2.05 to 3.85 % and Zn content ranged from 0.49 to 5.14 %. In 2 months, storage study the zinc followed a non-significantly decreasing trend in all treatments. Hence, it was concluded that T7 had the maximum ash content and sensory scores among all treatments, if investment employed it could bring a boon to therapeutic food product manufacturers.


AACC (American Association of Cereal Chemists). 2000. Approved methods of the AACC. St. Paul: AACC.

AOAC. 1997. Official Methods of Analysis, 18th edition, Association of Official Analytical Chemists, AOAC intranet. Gaithersburg, MD, USA.

Al-Hooti S, Sidhu JS, Al-Otaibi J, Al-Ameeri H, Al-Qabazard H. 1997 Date bars fortified with almonds, sesame seeds, oat flakes and skim mik powder. Plant Foods for Human Nutrition 51(2), 125-35.

Aigster A, Duncan SE, Conforti FD, Barbeau WE. 2011 Physicochemical properties and sensory attributes of resistant starch-supplemented granola bars and cereals. LWT-FoodScience and Technology 44(10), 2159-65.

Aparecida Damasceno K, Alvarenga Gonçalves CA, Dos Santos Pereira G, Lacerda Costa L, Bastianello Campagnol PC, Leal De Almeida P, Arantes‐Pereira L. 2016. Development of cereal bars containing pineapple peel flour. Journal of Food Quality 39(5), 417-24.

Aini N, Prihananto V, Wijonarko G, Sustriawan B, Dinayati M, Aprianti F. 2018 Formulation and characterization of emergency food based on instan corn flour supplemented by instan tempeh (or soybean) flour. International Food Research Journal 25(1).

Azmat Z, Durrani Y, Qazi IM, Ahmed I, Rasheed S. 2017. Effect of Antioxidants on Storage Quality of Apple Sucrose Bars. B. Life and Environmental Sciences165.

Barr SI, Murphy SP, Poos MI. 2002. Interpreting and using the dietary references intakes in dietary assessment of individuals and groups. Journal of the American Dietetic Association 102(6), 780-8.

Bhatt DA, Jha A. 2015. A study of incorporation of therapeutic values of wood apple (Feronia limoniaswingle) in fruitbar. Intermational Journal of Pharmaceutical Sciences and Research 6(10), 4398-4405.

Brown KH, Peerson JM, Rivera J, Allen LH. 2002. Effect of supplemental zinc on the growth and serum zinc concentrations of prepubertal children: a meta-analysis of randomized controlled trials. The American journal of clinical nutrition 75(6), 1062-71.

Chen L, Remondetto GE, Subirade M. 2006. Food protein-based materials as nutraceutical delivery systems. Trends in Food Science & Technology 17(5), 272-83.

Dary O, Hurrell R. 2006. Guidelines on food fortification with micronutrients. World Health Organization, Food and Agricultural Organization of the United Nations: Geneva, Switzerland.

Estevez AM, Escobar B, Ugarte V. 2000. Use of mesquite cotyledon (Prosopischilensis (Mol) Shuntz) in the manufacturing of cereal bars. Archivos latinoamericanos de nutrición 50(2), 148-51.

Garcia MC, Lobato LP, Benassi MD, Soares Júnior MS.2012. Application of roasted rice bran in cereal bars. Food Science and Technology 32(4), 718-24.

Gohari AA, Farhoosh R, Haddad KM. 2011.  Chemical composition and physicochemical properties of pumpkin seeds (Cucurbita pepo Subsp. pepo Var. Styriaka) grown in Iran.

Gutkoski LC, Bonamigo JMA, Teixeira DMF. Pedó I. 2006. Development of high-grade oat-based cereal bars of dietary fiber. Food Science and Hettiarachchi M, Liyanage C, Wickremasinghe R, Hilmers DC, Abrams SA. Prevalence and severity of micronutrient deficiency: a cross-sectional study among adolescents in Sri Lanka. Asia Pacific journal of clinical nutrition 15(1), 56.

Kanwal S, Raza S, Naseem K, Amjad M, Bibi N, Gillani M. 2015. Development, physico-chemical and sensory properties of biscuits supplemented with pumpkin seeds to combat childhood malnutrition in Pakistan. Pakistan Journal of Agricultural Research 28(4).

Kawade R. 2012. Zinc status and its association with the health of adolescents: a review of studies in India. Global health action 5(1), 7353.

Kajal MF, Wadud A, Islam MN, Sarma PK.2012. Evaluation of some chemical parameters of powder milk available in Mymensingh town. Journal of the Bangladesh Agricultural University 10(1), 95-100.

Khalid M, Pradyuman K. 2014. Oats as a functional food: a review. Universal Journal of Pharmacy 03(01), 14–20

Kim KS, Park SH. 2008. Anthrasesamone F from the seeds of black Sesamumindicum. Bioscience, biotechnology, and biochemistry 72(6), 1626-7.

King JC, Keen CL, Shils ME, Olson JA, Shike M, Ross CA. 2003. Modern nutrition in health and disease 9, 223 – 239.

Liu X, Zhou P, Tran A, Labuza TP. 2009. Effects of polyols on the stability of whey proteins in intermediate-moisture food model systems. Journal of agricultural and food chemistry 57(6), 2339-45.

Mourao LHE, Pontes DF, Rodrigues MCP, Brazil IM, Souza MA, Cavalcante MTB. 2009. Obtaining bars plum cashew cereals with high fiber content. Food and Nutrition 20(3), 427-433.

Munir M, Nadeem M, Qureshi TM, Jabbar S, Atif FA, Zeng X. 2016. Effect of protein addition on the physicochemical and sensory properties of fruit bars. Journal of Food Processing and Preservation 40(3), 559-66.

Mridula D, Kaur D, Nagra SS, Barnwal P, Gurumayum S, Singh KK. 2011Growth performance, carcass traits and meat quality in broilers, fed flaxseed meal. Asian-Australasian Journal of Animal Sciences 24(12), 1729-35.

Nadeem M, Haseeb M, Aziz Awan J. 2012. Development and physico-chemical characterization of apricot-date bars. Journal of Agricultural Research 50(3).

Nadeem M, Rehman SU, Mahmood Qureshi T, Nadeem Riaz M, Mehmood A, Wang C. 2018. Development, characterization, and flavor profile of nutrient dense date bars. Journal of food processing and preservation 42(10), e13622.

NNS. 2018. National Nutrition Survey Pakistan. Nutrition Wing, Cabinet Division, Government of Pakistan.

Padmashree A, Sharma GK, Srihari KA, Bawa AS. 2012. Development of shelf stable protein rich composite cereal bar. Journal of food science and technology 49(3), 335-41.

Peterson DM, Wesenberg DM, Burrup DE, Erickson CA. 2005. Relationships among agronomic traits and grain composition in oat genotypes grown in different environments. Crop Science 45(4), 1249-55.

Safdar MN, Mumtaz AM, Amjad M, Siddiqui N, Raza S, Saddozai AA. 2014. Quality of guava leather as influenced by storage period and packing materials. Sarhad Journal of Agriculture 30(2).

Shaheen B, Nadeem M, Kauser T, Mueen-ud-Din G, Mahmood S. 2013. Preparation and nutritional evaluation of date based fiber enriched fruit bars. Pakistan Journal of Nutrition 12(12), 1061-5.

Silva JS. 2012. Cereal bars made with flour pumpkin seed, Dissertation (Master in Agrochemistry). Federal University Of Lavras-MG.

Sotiles AR, Daltoé ML, de Lima VA, Porcu OM, da Cunha MA. 2015. Technological use of green banana and birdseed flour in preparing cookies. ActaScientiarum. Technology 37(4), 423-9.

Stevenson DG, Eller FJ, Wang L, Jane JL, Wang T, Inglett GE. 2007. Oil and tocopherol content and composition of pumpkin seed oil in 12 cultivars. Journal of agricultural and food chemistry 55(10), 4005-13.

Wapnir RA. 2000. Zinc deficiency, malnutrition and the gastrointestinal tract. The Journal of nutrition 130(5), 1388S-92S.

Youssef MK, Nassar AG, Fishawy FA, Mostafa MA. 2016. Assessment of Proximate Chemical Composition and Nutritional Status of Wheat Biscuits Fortified with Oat Powder. Assiut Journal of Agriculture Science 83-94.

Zebib H, Bultosa G, Abera S. 2015. Feb Physico-chemical properties of sesame (Sesamumindicum L.) varieties grown in Northern Area, Ethiopia. Agricultural Sciences 6(02), 238.