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Estimation of genetic diversity in Cupressus sempervirens growing in different ecological zones of Malakand Division, KP, Pakistan

Nazim Hassan, SyedFazal Wadood, Habib Ullah, Muhammad Zahoor, Murad Khan, Asif Khan, Muhammad Nisar

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Int. J. Biosci.8(5), 15-21, May 2016

DOI: http://dx.doi.org/10.12692/ijb/8.5.15-21

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Abstract

The nature and magnitude of geographic genetic diversity among 11 genotypes of Pakistani Cupressussempervirens was studied through Sodium DodecylSulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE).Five populations representing different ecological conditions were sampled. A total of five loci (bands) were detected in the collected germplasm of cupresses sempervirens genotypes. Only one locus (locus-1) was found monomorphic while the remaining were polymorphic loci. Out of polymorphic loci, locus-5 showed a high degree of intra-specific locus variation (ISLV) i.e. 54.54% followed by Locus-3 which indicated 36.36%ISLVin the collected germplasm of C. sempervirence genotypes. Intra specie locus contribution towards genetic dis-agreement (LCTGD) was 80%. It was found that the genotypes of each zone occupied separate cluster in the dendrogram.PCA was carried out to complement the cluster analysis information.SDS-PAGE profiling based on two-way cluster plotting successfully resolved the genotypes on the basis of zone distribution. For better understanding of the genetic structure of C. sempervirencefurther analysis using dominant molecular markers are recommended.

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Estimation of genetic diversity in Cupressus sempervirens growing in different ecological zones of Malakand Division, KP, Pakistan

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