Estimation of molecular diversity of Berberis lycium in three districts of Azad Jammu and Kashmir

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Research Paper 01/05/2018
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Estimation of molecular diversity of Berberis lycium in three districts of Azad Jammu and Kashmir

Rizwan Azim, Muhammad Ilyas, Shahid Iqbal Awan, Adnan Rasheed, Mustansar Shakil
J. Bio. Env. Sci.12( 5), 32-39, May 2018.
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Research study was carried in the Pant Genetic Resources Institute (PGRI), NARC Islamabad for the assessment of molecular diversity of Berberis lyciumin three districts of Azad Jammu and Kashmir. The present research describes the importance genetic diversity of medicinally important Berberis specie Berberberis lycium from great mountainous regions of AJK. Nine locations i.e; Hurnamaira, Datot, Topa, Tolipeer, Hullar, Morifurman Shah, Trarakhal, Baloch and Pullandri were selected on the basis of different altitudes and agroclimatic conditions and mature fruits were harvested from different barberry ecotypes. The matured harvested fruits were kept in the plastic pots and transported to Pant Genetic Resources Institute (PGRI), NARC Islamabad where they were subjected to -80 ˚C. Seeds were separated from the fruit pulp and used for protein analysis. SDS PAGE technique was used for the molecular characterization of selected ecotypes. Ecotypes Morifurman Shah and Tolipeer showed maximum genetic variation on the basis of molecular studies.


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