Evaluating biotoxicity of cypermethrin towards non-targeted organisms by using phytotoxic, cytotoxic and antimicrobial bioassays
Paper Details
Evaluating biotoxicity of cypermethrin towards non-targeted organisms by using phytotoxic, cytotoxic and antimicrobial bioassays
Abstract
Pesticides are inevitably used in the horticulture, aquaculture, and agriculture to prevent pests’ infestation and support systematic sustainability. A minute concentration of pesticides reaches the target community while rest of the volume either bulks-up in soil or reaches groundwater and aquatic systems through different channeling processes. Presence of pesticides is undesirable in these systems and may pose long term environmental hazards by interacting with the non-targeted communities in the vicinity. The current study was conducted to project the biotoxicity profile of an abundantly used pesticide ‘Cypermethrin’ by combining various toxicology parameters including Phytotoxicity, Cytotoxicity, Antibacterial and Antifungal activity assays. Evaluation of phytotoxicity of Cypermethrin showed a 50% reduction in radish seeds’ viability on exposure to 100ppm pesticide concentration. A significant decrease in the root and shoot length was recorded on 50 – 1000 ppm pesticide concentration. Cytotoxic profiling of Cypermethrin revealed detrimental effects of pesticide on the Artemia salina larvae, killing 50% of the population at a concentration of 11.161µg/L. Cypermethrin significantly inhibited growth of non-targeted bacterial species at a concentration of 10ppm and more, while showing drastic effect on the growth of Escherichia coli. Antifungal assay affirmed biotoxicity of pesticide towards fungal domain by reducing the growth of fungal species at a pesticide concentration of 10ppm and more with highest impact on the growth of Physarum polycephalum. By using a combination of novel and conventional model organisms, the current study reports biotoxicity of Cypermethrin which is an abundantly used pesticide in agricultural systems.
Abdel-Hafez S, El-Said A, Moharram A, Saleem A. 2010. Effect of two insecticides, Sparkill (25% Cypermethrin) and Tafaban (48% Chorpyrifos) on mycobiota of maize plants in Upper Egypt. Archives of Phytopathology and Plant Protection 43, 783-800. https://doi.org/10.1080/0323540086804
Atamanalp M, Keleş MS, Haliloğlu Hİ, Aras MS. 2002. The effects of cypermethrin (a synthetic pyrethroid) on some biochemical parameters (Ca, P, Na and TP) of rainbow trout (Oncorhynchus mykiss). Turkish Journal of Veterinary and Animal Sciences 26, 1157-1160.
Bajet C, Kumar A, Calingacion M, Narvacan T. 2012. Toxicological assessment of pesticides used in the Pagsanjan-Lumban catchment to selected non-target aquatic organisms in Laguna Lake, Philippines. Agricultural water management 106, 42-49. https:// doi.org/10.1016/j.agwat.2012.01.009
Bhalodia NR, Shukla VJ. 2011. Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: An ethnomedicinal plant. Journal of advanced pharmaceutical technology & research 2, 104-109. https://dx.doi.org/10.4103%2F2231-4040.82956
Bragança I, Lemos PC, Barros P, Delerue-Matos C, Domingues VF. 2018. Phytotoxicity of pyrethroid pesticides and its metabolite towards Cucumis sativus. Science of The Total Environment 619, 685-691. https://doi.org/10.1016/j.scitotenv.
Carriger JF, Rand GM, Gardinali PR, Perry WB, Tompkins MS, Fernandez AM. 2006. Pesticides of potential ecological concern in sediment from south Florida canals: an ecological risk prioritization for aquatic arthropods. Soil & Sediment Contamination 15, 21-45. https://doi.org/10.1080/ 15320380 500363095
Gilliom R, Barbash J, Crawford C, Hamilton P, Martin J, Nakagaki N, Nowell L, Scott J, Stackelberg P, Thelin G. 2006. Circular 1291. US Geological Survey 1992-2001.
González-Doncel M, Fernández-Torija C, Hinton D, Tarazona J. 2004. Stage-specific toxicity of cypermethrin to medaka (Oryzias latipes) eggs and embryos using a refined methodology for an in vitro fertilization bioassay. Archives of environmental contamination and toxicology 48, 87-98. https://doi.org/10.1007/s00244-003-0223-1
Grosch DS. 1967. Poisonig with DDT: Effect on Reproductive Performance of Artemia. Science 155, 592-593. DOI: 10.1126/science.155.3762.592
Hanley M, Whiting M. 2005. Insecticides and arable weeds: effects on germination and seedling growth. Ecotoxicology 14, 483-490. https://doi.org /10.1007/s10646-004-1353-6
Hua G, Guofeng C, Zhaoping L, Hua Z, Hong Y. 2009. Alteration of microbial properties and community structure in soils exposed to napropamide. Journal of Environmental Sciences 21, 494-502. https://doi.org/10.1016/S1001-0742(08)-6
Knisel W. 1993. Groundwater loading effects of agricultural management systems, version 2.10. UGACPES-BAED publication.
Li X, Ping X, Xiumei S, Zhenbin W, Liqiang X. 2005. Toxicity of cypermethrin on growth, pigments, and superoxide dismutase of Scenedesmus obliquus. Ecotoxicology and environmental safety 60, 188-192. https://doi.org/10.1016/j.ecoenv.2004.01.012
Liu T, Wang T, Sun C, Wang Y. 2009. Single and joint toxicity of cypermethrin and copper on Chinese cabbage (Pakchoi) seeds. Journal of hazardous materials 163, 344-348. https://doi.org/10.1016 /j.jhazmat.2008.06.099
Matthews G. 2015. “Pesticides: health, safety and the environment,” John Wiley & Sons.
Mian L, Mulla M. 1992. Effects of pyrethroid insecticides on nontarget invertebrates in aquatic ecosystems. Journal of agricultural entomology (USA).
Morandim A, Pin AR, Pietro NA, Alecio AC, Kato MJ, Youngcm, De Oliveira JE, Furlan M. 2010. Composition and screening of antifungal activity against Cladosporium sphaerospermum and Cladosporium cladosporioides of essential oils of leaves and fruits of Piper species. African Journal of Biotechnology 9, 6135-6139.
NCCLS 1993. Performance Standards for Antimicrobial Disc Susceptibility Tests. Approved Standard NCCLS Publication Villanova, PA, USA.
Nisharaj N, Thomas S, Helen P, Sree SJ, Suku S. 2012. Biodegradation of Cypermethrin. International Journal of Agriculture, Environment and Biotechnology 5, 1-4.
Obidola S, Ibrahim II, Yaroson A, Henry U. 2019. Phytotoxicity of Cypermethrin Pesticide on Seed Germination, Growth and Yield Parameters of Cowpea (Vigna unguiculata). Asian Journal of Agricultural and Horticultural Research p. 1-10. https://doi.org/10.9734/ajahr/2019/v3i229995
Omar S, Abdel-Sater M. 2001. Microbial populations and enzyme activities in soil treated with pesticides. Water, air, and soil pollution 127, 49-63. https://doi.org/10.1023/A:1005209516845
Rede D, Santos LH, Ramos S, Oliva-Teles F, Antão C, Sousa SR, Delerue-Matos C. 2016. Ecotoxicological impact of two soil remediation treatments in Lactuca sativa seeds. Chemosphere 159, 193-198. https://doi.org/10.1016/j.chemosph ere. 2016.06.002
Sánchez-Fortún S, Barahona M. 2005. Comparative study on the environmental risk induced by several pyrethroids in estuarine and freshwater invertebrate organisms. Chemosphere 59, 553-559. https://doi.org/10.1016/j.chemosphere.2004.12.023
Sethi BK, Pradhan S, Behera N, Sahoo SL. 2015. Effect of cypermethrin, a pyrethroid insecticide on dynamics of soil micro flora. J Appl Biol Biotechnol 3, 19-25. 10.7324/JABB.2015.3504
Shakir SK, Kanwal M, Murad W, ur Rehman Z, ur Rehman S, Daud M, Azizullah A. 2016. Effect of some commonly used pesticides on seed germination, biomass production and photosynthetic pigments in tomato (Lycopersicon esculentum). Ecotoxicology 25, p. 329-341. https://doi.org/ 10.1007 /s10646-015-1591-9
Shamsuddeen U, Inuwa A. 2013. Utilization of cypermethrin by bacteria isolated from irrigated soils. Bayero Journal of Pure and Applied Sciences 6, 19-22.
Sheng XM, Xiong L, LIU T, WANG Y. 2004. Toxicity of cypermethrin to Daphnia magna HB. Journal of Environmental Sciences 16, 770-771.
Slabbert J. 1986. Improved bacterial growth test for rapid water toxicity screening. Bulletin of environmental contamination and toxicology 37, 565-569.
Srivastava L. 2014. Determination of Pesticide Residues in Pulses Seed and Evaluation of their Phytotoxicity in Term of Germination and Early Seedling Growth by Hydroponic Culture. International Journal 2, 489-497.
Staley ZR, Harwood VJ, Rohr JR. 2015. A synthesis of the effects of pesticides on microbial persistence in aquatic ecosystems. Critical reviews in toxicology 45, 813-836.
Turker AU, Camper N. 2002. Biological activity of common mullein, a medicinal plant. Journal of ethnopharmacology 82, 117-125.
Werner I, Moran K. 2008. Effects of pyrethroid insecticides on aquatic organisms. ACS Publications Chapter 14. 310-334. 10.1021/bk-2008-0991.ch014
Willis KJ, Ling, N. 2004. Toxicity of the aquaculture pesticide cypermethrin to planktonic marine copepods. Aquaculture Research 35, 263-270.
Zhang W, Jiang F, Ou, J. 2011. Global pesticide consumption and pollution: with China as a focus. Proceedings of the International Academy of Ecology and Environmental Sciences 1(2), 125-144.
Maliha Ahmed, Naeem Ali, 2020. Evaluating biotoxicity of cypermethrin towards non-targeted organisms by using phytotoxic, cytotoxic and antimicrobial bioassays. Int. J. Biosci., 16(4), 514-523.
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