Evaluating the effects of elevated zinc concentrations on chlorophyll, reducing sugar, protein, prolein content and growth of wheat (Triticum aestivum)

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Evaluating the effects of elevated zinc concentrations on chlorophyll, reducing sugar, protein, prolein content and growth of wheat (Triticum aestivum)

Preeti Rai, Sangeeta Dayal
Int. J. Biosci.9( 6), 28-34, December 2016.
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Zinc is one of the essential micronutrients require to plants for growth and metabolism. Zinc is frequently used in fertilizer to promote growth and development of plants. The aim of the study is to analyze the impact of 100 ppm, 400 ppm and 800 ppm concentration of zinc on a physiological and biochemical aspects of the wheat. Spectrophotometric methods were used to determine the biochemical parameters while growth was measured manually through centimetre ruler. The results suggest that zinc affects growth parameters positively at lower concentration. Shoot growth increase at lower concentrations and reduced at highest concentration. While Root growth, enhanced with increase in concentration. Chlorophyll content increases with lowest concentration. Protein content shows16.6% increase at 100ppm then constant decline 16.7% at 400ppm and 50% decrease in protein content recorded at 800 ppm. Elevated zinc concentration reduces sugar content, minimum lower value of.03mg/g at 8000ppm and highest 1.8mg/g recorded in control. Prolein shows highest of 83.3% increase at 800ppm, 49.5% at 400ppm and 17.3% at 100ppm zinc concentration. The control shows the lowest value for prolein. It was observed that 100ppm concentration enhances wheat growth and biochemical parameters.


Aery NC, Sarkar S. 1991. Studies on the effect of heavy metal stress on growth  parameters of soybean. Journal of Environmental Biology 12(1), 15-24.

Alan SM. 1981. Influence of aluminium on plant growth and mineral nutrition of  barley. Communication in Soil Science and Plant Analysis, 12, 121-138.

Alia P, Saradhi P. 1991. Proline accumulation under heavy metal stress. Journal of Plant Physiology. 138(5), 554–558. http:/​/​dx.​doi.​org/10.1016/S0176-1617(11)80240-3

Ambler JE, Brown JC, Gauch HG. 1970. Effect of zinc on translocation of iron in  soybean plants. Plant Physiology 46, 320-323. http:/​/​dx.​doi.​org/​10.​1104/​pp.​46.​2.​320

Arnon DI. 1949.“Copper enzymes in isolated chloroplasts: polyphenoloxidase in Beta  vulgaris”,  Plant Physiology. 1949(24), 1-15. http:/​/​dx.​doi.​org/10.1104/pp.24.1.1

Ahmad  P, Sharma S, Srivastava PS. 2007. In vitro selection of NaHCO3 tolerant Cultivars of Morus alba (Local and Sujanpuri) in response to morphological and   biochemical parameters. Horticulture Science (Prague) 34, 114–122.

Bates LS, Waldren RP, Teare ID. 1973. Rapid determination of the free proline in water stress studies. Plant Soil 38, 205-208. http:/​/​dx.​doi.​org/10.1007/BF00018060

Bameri M, Abdolshahi R, Mohammadi-Nejad GH,Yousefi KH, Tabatabaie SM.  2012. Effect of different microelement treatment on wheat (Triticum aestivum) growth and yield. International Research. Journal of  Applied Basic Science, 3 (1), 219-223.

Bassi R, Sharma SS. 1993. Proline accumulation in wheat seedlings exposed to zinc and copper Phytochemistry 33, 1339–1342.

Brown JC, JAmbler JE,  Chaney RL, Foy CD. 1972. Differential responses of plant genotypes to micronutrients. In: Mortved, J.J., P.M. Giordano and W.L. Lindsay (Eds.), Micronutrients in Agriculture. Soil Science Society of America Inc., Madison, 389-418.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analytical Biochemistry  72, 248-254. http:/​/​dx.​doi.​org/10.1016/0003-2697(76)90527-3.

Crooke WM, Inkson RHE. 1955. The relationship between nickel toxicity and   major nutrient supply. Plant Soil, 6, 1-15.

Chaney RL. 1993. Zinc phytotoxicity. In: Robson A.D. (Ed.), Zinc in soil and plants.  Kluwer Academic Publishers, Dordrecht, the Netherlands: 135–150.

Cherif J, Mediouni C, Ammar WB, Jemal F. 2001. Interaction of zinc and cadmium toxicity in their effect on growth and in antioxidative system in tomato plant   (Solarium lycopersium). Journal of environmental science 23, 837-844.

Devlin RM. 1975. Plant physiology, 3rd Edn., Litton Educational Publishing, Inc., New  York.

Ebbs SP, Kochaian ALU. 1998. Phytoextraction of zinc by oat (Avena stiva), Barley (Hordeum vulgare) Indian Mustard (Brassica juncea). Environment Science and Technology. 32, 802-806.

Foy CD. 1988. Plant adaptation to acid, aluminum soils.  Communication in Soil Science and Plant Analysis 19, 959-987.

Granick S. 1951. Biosynthesis of chlorophylls and related pigments. Annual Review of Plant Physiology, 2, 115-144. http:/​/​dx.​doi. org/10.1146/annurev.pp.02.060151.000555

Greger M, Lindberg S. 1986. Effects of Cd2+ and EDTA on young sugarbeets (Beta vulgaris  L.) Cd2+ uptake and sugar accumulation. Physiologia Plantarum 66, 69-74. http:/​/​dx.​doi.​org/10.1111/j.13993054.1986.tb01235.x

Hewitt EJ, Robb DA, Pierpoint WS. 1983. Metals and micronutrients: uptake and utilization by plants, Academic Press, London, 277–300 p.

Kastori R, Petrovic M, Petrovic N. 1992. Effect of excess lead, cadmium, copper and zinc on water relations in sunflower. Journal of Plant Nutrition, 15(11), 2427-2439.

Kuznetsov VV, Shevyakova NI. 1997. Stress responses of tobacco cells to hightemperature and salinity. Proline accumulation and phosphorilation of polypeptides.Physiologia Plantarum 100, 320- 326. http:/​/​dx.​doi.​org/10.1111/j.13993054.1997.tb04789.x

Manivasagaperumal R, Balamurugan S, Thiyagarajan G, Sekar J. 2011. Effect of Zinc on Germination, Seedling Growth and Biochemical Content of Cluster Bean (Cyamopsis tetragonoloba (L. Taub). Current Botany 2, 11-15.

Marschner H. 1995. Mineral Nutrition of Higher Plants. Second edition. London: Academic Press, 889 p. http://dx.doi.org/10.1006/anbo.1996.0155

Mayz DMJ,  Cartwright PM. 1984. The effects of pH and aluminium toxicity on the growth and symbiotic development of cowpea (Vigna unguiculata). Plant  Soil, 80, 423-430. 

Nag P, Paul AK, Mukherjee S. 1981. Heavy metal effects in plant tissues   involving  chlorophyll, chlorophyllase, hill reaction activity and gel electrophoresis patterns of  soluble proteins. Indian Journal of Experimental. Biology 19, 702.

Ross AF. 1959. Dinitophenol method for reducing sugar The Avi. Publishing company, Frist edition, Wesport.

Samarakoon AB, Rauser WE. 1979. Carbohydrate levels and photostimulate export from leaves of Phaseolus vulgaris exposed to excess cobalt, nickel and zinc. Plant Physiology 63, 1165-1169.

Shrotri CK, Tewari MN, Rathorem VS. 1979. Effect of zinc on chlorophyll, sugars and starch contents in maize (Zea mays L.). Indian Journal of Experimental Biology 17, 58-60.

Singh D, Nath K, Sharma YK . 2007. Response of wheat seed germination and seedling growth under copper stress. Journal of Environmental Biology, 28, 409- 414.

Song A, Li P, Li Z, Fan F, Nikolic M, Liang Y. 2011. The alleviation of zinc toxicity by silicon is related to zinc transport and antioxidative reactions in rice. Plant and Soil 344, 319-333. http:/​/​dx.​doi.​org/10.1007/s11104-011-0749-3

Shier WT. 1994. Metals as toxins in plants, Journal of Toxicology.-Toxin Reviews. 13, 205–216. http://dx.doi.org/10.3109/15569549409089960

Waldren RP, Teare ID. 1973. Rapid determination of the free proline in water stress studies. Plant and Soil, 38, 205-208.

Zengin FK, Kirbag S. 2007. Effects of copper on chlorophyll, proline, protein and   abscisic acid level of sunflower (Helianthus annuus L.) seedlings. Journal of Environmental Biology 28(3), 561-566.