Evaluation of alpha-fetoprotein in patient with chronic hepatitis C infection by using ELFA technology

Paper Details

Research Paper 01/10/2016
Views (247) Download (8)

Evaluation of alpha-fetoprotein in patient with chronic hepatitis C infection by using ELFA technology

Bakht Biland, Muhammad Waqar, Muhammad Wasim, Zobaria Rehman, Agha Asad Noor, Shahzeray Mukhtar, Qazi Shah Rukh, Noor Ul Akbar
Int. J. Biosci.9( 4), 176-181, October 2016.
Certificate: IJB 2016 [Generate Certificate]


Hepatitis known as inflammation of liver is caused by some known viruses, alcohol, drugs and some other infection. Most common hepatitis infections in humans are Hepatitis A, B and C. Chronic HCV infection may lead to liver cirrhosis and Hepatocellular carcinoma (HCC). Alfa feto protein’s concentration in patient’s serum is useful utility to diagnose HCC. The current study was conducted at Genome Research Centre Lahore for the measurement of AFP concentration in HCV positive patients of Punjab and Khyber Paktun Khwa (KPK) Province of Pakistan. Total of 172 patients, atleast 60 from each province were screened for Alfa feto protein concentration in their blood by using modern ELFA technology that is most rapid, specific and sensitive method. Results show high percentage rate of HCC in Punjab as compared to KPK Province. Age wise Results analyse that age group >60 years show very high percentage rate of HCC and in male patients s compared to females. It is concluded that due to prolong HCV infection, patients of age group >60 are at high risk of liver cirrhosis and HCC. It is also concluded that the concentration of AFP in patient’s blood is useful test for diagnosis of HCC and to monitor the patient’s condition.


Abelev GI, Lazarevich NL. 1996. Alpha-fetoprotein (AFP): solved and unsolved problems. McGill journal of medicine 2, 127–34.

Ahmad I, Khan SB, Rahman HU, Khan MH, Anwar S. 2006. Frequency of Hepatitis B and Hepatitis C among cataract patients. Gomal Journal of Medical Science 4, 61–64.

Ahmedin J, Taylor M, Ram CT. 2004. A New Section in Cancer Offering Timely and Targeted information. Can J Clin. 54, 23–25.

Beasley RP, Hwang LY, Lin CC, Chien CS. 1981. Hepatocellular carcinoma and hepatitis B virus. A prospective study of 22 707 men in Taiwan. Lancet 2, 1129-33.

Benvegnu L, Fatovich G, Noveta F. 1994. Co-current hepatitis B and C virus infection and risk of HCC cirrhosis. Cancer 74, 2442-7.

Daniele B, Bencivenga A, Megna AS, Tinessa V. 2004. Alpha-fetoprotein and ultrasonography screening for hepatocellular carcinoma. Gastroenterology 127, 108-12.

Davila JA, Morgan RO, Shaib Y, McGlynn KA, El-Serag HB. 2004. Hepatitis C infection and the increasing incidence of hepatocellular carcinoma: a population-based study. Gastroenterology 127, 1372-80.

Davila JA, Morgan RO, Shaib Y, McGlynn KA,  El-Serag HB. 2004. Hepatitis C infection and the increasing incidence of hepatocellular carcinoma: a papulation based study. Gastroenterology 127, 1372-80.

Engelhardt NV, Poltoranina VS, Yazova AK. 1937. Localization of alpha-fetoprotein in transplantable murine teratocarcinomas. International Journal of Cancer 11, 448–5.

Isselbacher K, Dienstag J. 1998. Tumors of the liver and biliary tract: principles of internal medicine. Fauci Braunwald Wilson et al (Eds 14th), Mc Graw Hill Companies, Italia, part six 578-80.

Johnson PJ. 2001. The role serum alpha- fetoprotein estimation in the diagnosis and management of hepatocellular carcinoma. Clinics in Liver Disease 5(1), 145–59. 15.

Choo QL. 1989. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244, 359–362.

Perrillo RP. 1990. Factors influencing response to interferon in chronic hepatitis B: implications for Asian and western populations. Herpetology 12, 1433–5.

Ryder S, Beckingham I. 2001. ABC of diseases of liver, pancreas, and biliary system: Acute hepatitis. British Medical Journal 322, 151–153.

Sherman M. 2001. Alphafetoprotein: an obituary. Journal of Hepatology 34, 603-5.

Tsukuma H. 1993. Risk factors for hepatocellular carcinoma among patients with chronic liver disease. N England Journal of Medicine 328, 1797-801.

Francioni S, Pastore M. 1989. Alpha-fetoprotein and acute viral hepatitis type B. The J nuclear medicine and allied sciences 33, 103-6.

Li P, Wang SS, Liu H, Li N, McNutt MA, Li G. Elevated serum alpha fetoprotein levels promote pathological progression of hepatocellular carcinoma. World J Gastroenterol 2011; 17, 4563-71.

Baig. 2009. Hepatocellular carcinoma (HCC) and Diagnostic significance of α -fetoprotein (AFP). J Ayub Med Coll Abbottabad, 21(1), 72-75.