Evaluation of Anti-oxidant and Anti-ulcerogenic activity of Cannabis Sativa extract against aspirin-induced gastric ulcer in Rabbits

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Evaluation of Anti-oxidant and Anti-ulcerogenic activity of Cannabis Sativa extract against aspirin-induced gastric ulcer in Rabbits

Shafaq Naeem, Bilal Aslam, Zia-ud-Din Sindhu, Mashkoor Mohsin, Asif Hussain, Rida Siddique, Faheem Ahmed
Int. J. Biosci.15( 4), 462-470, October 2019.
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Cannabis sativa is an herbaceous perineal plant which has been used in traditional medicine for its anti-inflammatory, analgesic and sedative properties. In this study, the anti-oxidant and gastroprotective properties of C. sativa ethanolic extract were evaluated against aspirin-induced gastric lesions. For this purpose, thirty healthy rabbits were selected and divided into five groups (n=6) as follows: (I) normal saline, (II) aspirin alone (150mg/kg*day), (III) omeprazole (20mg/kg*day) plus aspirin, (IV and V) C. sativa extract (10mg/kg*day and 20mg/kg*day) with aspirin. All treatments were given orally for 14 days. Gastric ulcer parameters including ulcer index, ulcer score, gastric acid volume, total acid output and pH were assessed. Blood samples were collected to determine oxidative stress parameters (TOS, TAC, MDA and CAT). C. sativa exhibited significant (p≤0.01) antiulcer effect in comparison with aspirin alone treatment. Histopathological analysis revealed normalization of gastric mucosa in C. sativa treated animals. C. sativa administration showed a reduction in oxidative stress parameters (TOS and MDA) and increased anti-oxidants (TAC and CAT) level. Moreover, C. sativa, particularly at 20mg/kg, showed 70.66% ulcer protection which was comparable to 72.85% with omeprazole. Collectively, this study suggested that C. sativa has antioxidant and antiulcer properties.


Abdel-Salam OME, Amany AS, Eman RY, Fatma AM. 2018. Preventive effects of cannabis on neurotoxic and hepatotoxic activities of malathion in the rat. Asian Pacific Journal of Tropical Medicine 11(4), 272-279. https://doi.org/10.4103/1995-7645

Akhtar AH, Kamal UA. 1995. Anti-ulcerogenic evaluation of the methanolic extracts of some indigenous medicinal plants in aspirin-ulcerated rats. Journal of Ethnopharmacology 46(1), 1-6. https://doi.org/10.1016/0378-8741(94)01220-T.

Andre CMJean-Francois HGea G. 2016. Cannabis sativa: The Plant of the Thousand and One Molecule. Frontiers in Plant Science 7, 7-19. https://doi.org/10.3389/fpls.2016.00019.

Aslam B, Wafa M, Ijaz J, Faqir M, Tanweer K, Junaid AK, et al. 2013. Gastroprotective effect of Berberis vulgaris seeds against gastric ulcer induced by aspirin in male adult albino mice. Indo American Journal of Pharmaceutical Research 3(6), 4518-4527.

Asnaashari S, Siavash D, Yousef J. 2018. Gastroprotective effects of herbal medicines (roots). International Journal of Food Properties 21(1), 902-920. https://doi.org/10.1080/10942912.2018.1473876.

Baigent C, Blackwell L, Collins R, Emberson J, Godwin J, Peto R, et al. 2009. Aspirin in the primary and secondary prevention of vascular disease: Collaborative meta-analysis of individual participant data from randomized trials. Lancet 373, 1849-1860. https://doi.org/10.1016/S0140-6736.

Bancroft JD, Gamble M. 2002. Theory and Practice of Histological Techniques, 5th ed. Edinburgh; Churchill Livingstone.

Burke A, Smyth E, Fitzgerald GA. 2006. Analgesic-Antipyretic Agents. In: Goodman and Gilman, Pharmacological Bases of Therapeutics. 11th ed. New York; McGraw-Hill. p. 671-715.

El-Ghffar EAA, Eman ASSafia MS, Omayma AE, Thomas E. 2018. The protective role of Ocimum basilicum L. (Basil) against aspirin-induced gastric ulcer in mice: Impact on oxidative stress, inflammation, motor deficits and anxiety-like behavior. Food and Function 9(8), 4457-4468. https://doi.org/10.1039/c8fo00538a.

Erel O. 2004. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry 37(4), 277-285. https://doi.org/10.1016/j.clinbiochem.2003.11.015.

Erel O. 2005. A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry 38(12), 1103-1111. https://doi.org/ 10.1016/j.clinbiochem.2005.08.008.

Frassinetti S, Eleonora M, Leonardo C, Morena G, Vincenzo L, Lorenza B, et al. 2018. Nutraceutical potential of hemp (Cannabis sativa L.) seeds and sprouts. Food Chemistry 262, 56-66. https://doi.org/10.1016/j.foodchem.2018.04.078.

García-Rayado G, Mercedes N, Angel L. 2018. NSAID induced gastrointestinal damage and designing GI-sparing NSAIDs. Expert Review of Clinical Pharmacology 11(10), 1031-1043. https:// doi.org/10.1080/17512433.2018.1516143.

Goth L. 1991. A simple method for determination of serum catalase and reversion of reference range. Clinica Chimica Acta 2, 143-151. https://doi.org /10.1016/0009-8981(91)90067-M.

Gregory M, Divya B, Revina AM, Viji MMH, Kalaichelvan VK, Palanivel V. 2013. Anti-ulcer activity of Ficus religiosa leaf ethanolic extract. Asian Pacific Journal of Tropical Biomedicine 3(7), 554-556. https://doi.org/10.1016/S2221-1691(13)60112-4.

Guide for the Care and Use of Laboratory Animals. 2011. The National Academies Press. 8th ed. Washington (DC) USA.

Hooi JKYLai WYNg WKSuen MMYUnderwood FETanyingoh D. et al. 2017. Global prevalence of Helicobacter pylori Infection: Systematic Review and Meta-Analysis. Gastroenterology 153(2), 420429. https://doi.org /10.1053/j.gastro.2017.04.022.

Jaikumar S, Ramaswamy S, Asokan BR, Mohan T, Gnanavel M. 2010. Anti-ulcer activity of methanolic extract of Jatro phacurcas (Linn.) on aspirin-induced gastric lesions in wistar strain rats. Research Journal of Pharmaceutical Biological and Chemical Sciences 1, 886-897.

Kaner J, Lapidot T. 2001. The stomach as a bioreactor, Dietary lipid peroxidation in the gastric fluid and the effects of plant-derived antioxidants. Free Radical Biology and Medicine 31(11), 1388-1395. https://doi.org/10.1016/S0891-5849(01)0071.

Kavitt RT, Anna ML, Adjoa AY, Ian MG. 2019. Diagnosis and Treatment of Peptic Ulcer Disease. The American Journal of Medicine 132(4), 447-456. https://doi.org/10.1016/j.amjmed.2018.12.009. 

Kulkarni SK, Vallabh P. 2002. Hand Book of Experimental Pharmacology. New Delhi. p. 149-150.

Lanas A, Francis KLC. 2017. Peptic ulcer disease. Lancet 390(10094), 613-624. https://doi.org/10.

Longo DL, Fauci AS, Kasper DL, Hauser SL, Jameson JL, Loscalzo J. 2012. Harrison’s Principles of Internal Medicine. In: Valle JD, Ed. Peptic ulcer disease and related disorders. New York; McGraw-Hill. p. 683-688.

Miranda MA, Lemos M, Cowart KA, Rodenburg D, McChesney JD, Radwan MM, et al. 2015. Gastroprotective activity of the hydroethanolic extract and isolated compounds from the leaves of Solanum cernuum Vell. Journal of Ethnopharmacology 172, 421-429. https://doi.org.

Mirje MM, Zaman SU. 2014. Evaluation of the antiulcer activity of Ocimum sanctum L. in indomethacin-induced gastric ulcers in albino rats. International Journal of Life Sciences Biotechnology and Pharma Research 3(1), 274-279. https://doi.org /10.20959/wjpr20179-9196.

Mukherjee M, Bhaskaran N, Srinath R. 2010. Anti-ulcer and antioxidant activity of Gut Gard. Indian Journal of Experimental Biology 48, 269-274.

Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxide in animal tissue by the thiobarbituric acid reaction. Analytical Biochemistry 95, 351-358. https://doi.org/10.1016/0003-2697(79)90738-3.

Omar AS. 2016. Gastric acid inhibitory and gastric protective effects of cannabis and cannabinoids. Asian Pacific Journal of Tropical Medicine 9(5), 413-419. http://dx.doi.org/10.1016/j.apjtm.2016.04.021.

Raju D, Ilango K, Chitra VI, Ashish K. 2009. Evaluation of the anti-ulcer activity of methanolic extract of Terminalia chebula fruits in experimental rats. Journal of Pharmaceutical Sciences and Research 1, 101-107.

Saha L, Alka B, Amitava C. 2016. Gastroprotective effect of bezafibrate, a peroxisome proliferator-activated receptor α agonist and its mechanism in a rat model of aspirin-induced gastric ulcer. Advances in Digestive Medicine 3(3), 101-110. https://doi.org/10.1016/j.aidm.2016.04.001.

Sarri GLGrigg SEYeomans ND. 2019. Helicobacter pylori and low-dose aspirin ulcer risk: A meta-analysis. Journal of Gastroenterology and Hepatology 34(3), 517-525. https://doi.org/10.1111.

Schubert ML. 2017. Physiologic, pathophysiologic, and pharmacologic regulation of gastric acid secretion. Current Opinion in Gastroenterology 33(6), 430-438. https://doi.org/10.1097/MOG.00.

Silva DM, José LRM, Danillo RO, Iziara FF, Daiany PBS, Fernanda CAS. 2018. Effect of allantoin on experimentally induced gastric ulcers: Pathways of gastroprotection. European Journal of Pharmacology 821, 68-78. https://doi.org/10.1016.

Steel RGD, Torrie GH, Dickey DA. 1997. Principles and procedures of statistics. New York; McGraw-Hill. p. 1746-1762.

Suheryani I, Yujuan L, Rongji D, Xiujie L, Shoaib A, Song J, 2017. Gastroprotective effects of Dregea sinensis Hemsl. (Daibaijie) against aspirin-induced gastric ulcers in rats. International Journal of Pharmacology 13(8), 1047-1054. https://doi.org/10.3923/ijp.2017.1047.1054.

Vogel HG. 2002. Drug Discovery and Evaluation of Pharmacological Assay. 2nd ed. Germany; Springer. p. 759-767.

Wallace JL. 2013. Mechanisms, prevention and clinical implications of nonsteroidal anti-inflammatory drug-enteropathy. World Journal of Gastroenterology 19(12), 1861-1867. https://doi.org.

Zhou Y, Shanshan W, Hongxiang L, Peihong F. 2018. Chemical constituents of hemp (Cannabis sativa L.) seed with potential anti-neuroinflammatory activity. Phytochemistry Letters 23, 57-61. https://doi.org/10.1016/j.phytol.2017.11.013.