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Evaluation of Antiangiogenic, Cytotoxic and In vitro Antiproliferative Activities of Alpinia galanga Leaves Ethanol Extract

Research Paper | February 1, 2022

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Diomerl Edward B. Baldo, Ronnel A. Dioneda Jr.

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Int. J. Biosci.20( 2), 187-198, February 2022

DOI: http://dx.doi.org/10.12692/ijb/20.2.187-198


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Medicinal plants are an important source of natural treatments for a multitude of chronic illnesses like cancer. In this study, ethanol extract from the leaves of Alpinia galanga (AGEE) was evaluated for its anticancer potential. Chorioallantoic membranes (CAM), Tripneustes gratilla fertility, and MTT assays were conducted to investigate the angiangiogenic, cytotoxic and anti-proliferative activities, respectively. Standard phytochemistry tests were conducted to identify the bioactive compounds present. The CAMs treated with AGEE demonstrated signs of vascularity assault and significantly lowered blood vessel formation, branching point number and branching point density (p<0.05). The extracts also exhibited cytotoxicity with a significant reduction in the number of viable sea urchin zygotes as compared to control (p<0.05). Finally, AGEE portrayed evidence of antiproliferative activity against HCT-116 with pronounced growth inhibition of 53.01% and IC50 of 65.25 µg/ml. A less striking effect in MCF-7 was signified by the minimal inhibitory rate of 12.41% and IC50 of 101.4 µg/ml. AGEE showed positive indications for metabolites of flavonoids, sterols and tannins. Total phenolic content (TPC) was quantified at 36.71 gallic acid equivalents (GAE/g). The results suggest the potential of A. galanga as a reasonable prospect in the development of natural product-derived anticancer drugs.


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Evaluation of Antiangiogenic, Cytotoxic and In vitro Antiproliferative Activities of Alpinia galanga Leaves Ethanol Extract

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