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Evaluation of antibacterial activity of plant based silver nanoparticles in Synergism with Antibiotics

Research Paper | September 1, 2018

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Faisal Rasheed Anjum, Sidra Anam, Sajjadur Rahman, Ashiq Ali, Ahsan Naveed, SadafNaz

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Int. J. Biosci.13( 3), 250-261, September 2018

DOI: http://dx.doi.org/10.12692/ijb/13.3.250-261


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Plant derived silver nanoparticles might be a possible replacement of antibiotics in order to treat multi drug resistant bacterial infections, hence could be the answer to antibiotic resistance. The current study deals with the biosynthesis of silver nanoparticles (AgNPs) and determination of their bactericidal activity against Staphylococcus aureus and Streptococcus pneumoniae. The reduction of silver nitrate was indicated by characteristic change of colour from light yellowish to deep brown and finally colloidal brown. Spectrophotometer analysis showed that maximum absorption was observed at 450 nm. Silver nanoparticles with crystalline structure having 19 nm domain size was observed. The surface charge of silver nanoparticles was found to be varying with pH of the environment. Minimal inhibitory concentration (MIC50 and MIC90) against Staphylococcus aureus was 2.66 & 5.31 µg/100 µl, while MIC50 and MIC90 against Streptococcus pneumoniae were found to be 5.31 & 1.06×101/100 µl, respectively. Disc Diffusion Method has revealed that biosynthesized AgNPs when used in synergism with antibiotics have significantly larger zone of inhibition as compared to AgNPs alone. Briefly, this research has provided a simple and environment friendly method for production of Ag particles as compared to complex chemical method. Moreover, green synthesized particles formulation showed a significant antibacterial activity against both test strains, whereas combinations of AgNPs and antibiotics have a better bactericidal effect.


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Evaluation of antibacterial activity of plant based silver nanoparticles in Synergism with Antibiotics

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